Báo cáo khoa học: Utility of epimerization domains for the redesign of nonribosomal peptide synthetases pdf

... to utilize E -domains for the redesign of non- ribosomal peptides. The main issue to be addressed was if the substrate specificity of E domains is concordant with the specificity of the module (activated ... enzymes the results obtained on the substrate specificity of E domains could pave the way to engineering of biosynthesis of valuable non- ribosomal pepti...

Ngày tải lên: 30/03/2014, 20:20

... assessment of the trajec- tory of interconversion rates as a function of time of cold exposure, (b) comparison of the magnitudes of the various interconversion rates, and (c) lineup of the accessions ... & Stitt M (1993) Reg- ulation of the expression of rbcs and other photosyn- thetic genes by carbohydrates – a mechanism for the sink regulation of photos...

Ngày tải lên: 14/02/2014, 22:20

... structures and the relative ease of synthesis, makes them particularly useful templates for the design of optimized synthetic peptides for the subtype characteriza- tion of nAChRs. Acknowledgements A. ... in a cloning approach making use of the high conservation of the 3¢ untranslated region and the intron preceding the sequence of the a-prepropeptide [39]. Th...

Ngày tải lên: 19/02/2014, 12:20

... sequence of POS respectively as follows: nn wwwwE 121 − = nn ttttT 121 − = Where n is the number of words in the sentence, i t is the POS tag of the word i w . Given E, the result of the baseNP ... that the two steps are integrated together, rather that separated as in the other approaches. Let us now examine the two steps more closely. 2.3 Determining the N...

Ngày tải lên: 08/03/2014, 05:20

... can therefore postulate that, when the segment beyond W140 was removed, the secondary structure of the protein disintegrated. Using the peak at 222 nm of the wild-type protein as an index of helical ... that the apparent association constant of SNase(1–126) bound to pdTp in the presence of Ca 2+ is approxi- mately threefold lower than that of the wild-type pro- tei...

Ngày tải lên: 20/02/2014, 01:20

... random selections of a subset of the full training set. The sizes of the partitions are shown in Table 1, together with the resulting percentage of out -of- vocabulary (OOV) words in the develop- ment ... reliable (deter- mined by the number of occurrences in the train- ing data); otherwise, the model backs off to the next lower-order n-gram distribution. For th...

Ngày tải lên: 22/02/2014, 02:20

... native a-helices of the N-terminal 185 residues of the functional domain of the HA2 subunit of the influenza virus hemagglutinin protein and to detect conformational heterogeneity of the protein within ... of toxicity for cells, independently of the nature of the recombinant protein. Energy depletion is the most immediate result and is due to both the overpr...

Ngày tải lên: 06/03/2014, 00:20

... disruption of the homeostasis between food intake and energy expendi- ture, and therefore factors affecting these processes are the focus of extensive research targeting the develop- ment of effective ... cell-cycle regulation The mode of action of RMI1 in the regulation of energy homeostasis might be based on the regulation of adipocyte hyperplasia. Studies have sho...

Ngày tải lên: 06/03/2014, 01:20

... mixture was incubated on ice for 10 min without antibody or for 20 min with antibody in the absence of the radiolabeled probe, and then for 30 min at 30 °C in the presence of the radiolabeled probe, ... & DePaoli-Roach AA (1996) High complexity in the expression of the B¢ subunit of protein phospha- tase 2A0. Evidence for the existence of at least seven nov...

Ngày tải lên: 06/03/2014, 02:20

... cells to express either the wild-type or mutant proteins, and incubated the cells in the presence or absence of tunicamycin. Drug treatments resulted in the accumulation of forms of the mutant proteins ... mutant forms of HFE and incubated the cells in the presence and absence of tunicamycin. Drug treatment resulted in the accumulation of an unglycosylated lower mo...

Ngày tải lên: 06/03/2014, 22:21