Báo cáo khoa học: DNA sequence specificity of triplex-binding ligands docx

... Quantitative analysis of the concentration dependence of this footprint (Fig. 3D, d) yields a C 50 value of 1.0 ± 0.2 l M . Similar experiments in the presence of the triplex-binding ligands are shown ... presence of 10 l M ligands (Table 1). Oligos 3 and 4. Fig. 5 shows the interaction of oligo 4 (TCTCTCTCT) with its target site on tyrT(35–59) in the presence and absence o...

Ngày tải lên: 30/03/2014, 20:20

... ue to the presence of an arginine (Arg52) in ICAM-4, instead of the critical ICAM-1 glutamic acid (Glu34) which coordinated the I/A domain Mg 2+ . Regardless of the connection of I/A domains to 17 a ... presence of both Ca 2+ and Mg 2+ is required for maximal binding of ICAM-4 to the I domain of b 2 integrins [10]. This is in line with our preliminary observation showing that bi...

Ngày tải lên: 23/03/2014, 13:20

... genomic structure and identification of transcription start site of human KCTD10 To determine the genomic structure of human KCTD10, a full-length cDNA sequence of human KCTD10 was used to search ... Alignment of the 5¢-flanking sequences upstream of the start codon of human and mouse KCTD10. Fig. 5. In vivo ChIP assays of Sp1.1 and AP-2.2 binding sites in the proximal promote...

Ngày tải lên: 30/03/2014, 02:20

... l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ATP-c-S; (4) with 1.25 lM of OsDmc1 in presence of ... resulting in stretching of DNA by 1.5 times its original length. This conformational change in DNA is hypothesized to facilitate homology search in the double stranded DNA...

Ngày tải lên: 19/02/2014, 07:20

... of parent node in dependency tree. c-word: word of child node. p-pos: POS of parent node. c-pos: POS of child node. p-pos+1: POS to the right of parent in sentence. p-pos-1: POS to the left of ... parent of x j . T = {(x t , y t )} T t=1 denotes the training data. We follow the edge based factorization method of Eisner (1996) and define the score of a dependency tree as the s...

Ngày tải lên: 20/02/2014, 15:20

... instance of this method based on identity of words in the variation contexts, so-called variation n-grams, successfully detects a variety of errors in the WSJ corpus. The usefulness of the notion of ... table reports the level of variation in the WSJ across identical contexts of different sizes. In the next section we turn to the issue of detecting those occurrences of a var...

Ngày tải lên: 22/02/2014, 02:20

... maximized when we rely on syntactic rules defined in terms of part -of- speech at the cost of a high degree of ambiguity. Thus, the goal of maximizing the parsing coverage while minimizing the ... results of the technique on the basis of two sets of unseen test data. In section 5 we discuss system engineering issues to accommodate the proposed technique, i.e., integration...

Ngày tải lên: 22/02/2014, 03:20

... model the devel- opment of meaning of words as a function of in- creasing exposure to language, and ultimately - the degree to which the meaning of words at each stage of exposure resemble their ... training corpora of the two models have in common (recall that the adult corpus is a superset of each of the intermedi- ate corpora). The result of the Procrustes Align- ment...

Ngày tải lên: 07/03/2014, 22:20

... TYPE OF CONCEPTUAL OPERATIONS ? The conceptual operations of NL we intend to interpret are, for instance, Indlvldustions of objects, evaluations of objects, evaluations of properties of objects, ... kinds of memory, etc. Let us introduce a new example. The adjective is one of the more conlplex phenomena of NL which cannot be reduced to the notion of predicate sinc...

Ngày tải lên: 18/03/2014, 02:20

... model of negotia- tion through offers and counter-offers between two players with a public benefit constraint. The model consists of (1) two players repre- senting the opposing sides, (2) a set of ... idea of the proof is to construct a pair of strategies where each side attempts to pull the pub- licly accepted frame in the direction of its peak utility point. We show, assuming the...

Ngày tải lên: 30/03/2014, 21:20