Báo cáo khoa học: Cytosol–mitochondria transfer of reducing equivalents by a lactate shuttle in heterotrophic Euglena docx

... different preparations. Fig. 3. Intracellular concentrations of L -lactate and D -lactate in Euglena. (A) [ L -lactate] . (B) [ D -lactate] . Cultures with glutamate/malate (glu/mal) (j), DL -lactate (s), ... the latter enzyme [2], resulting in a lower intracellular level of D -lactate than of L -lactate. Most of the lactate formed remained trapped intracell- ularl...

Ngày tải lên: 30/03/2014, 20:20

... cofactor protein S. FVa consists of a 105 kDa heavy (A1 and A2 domains) and a 71–74-kDa light (A3 , C1, and C2 domains) chain which are noncovalently associated. During APC-catalyzed inactivation of FVa, ... (1997) Anticoagulant synergism of heparin and activated protein C in vitro. Role of a novel anticoagulant mechanism of heparin, enhancement of inactivation of facto...

Ngày tải lên: 19/02/2014, 13:20

... Miyazaki A, Saito M, Adachi T, Mizoue K, Hanada K & Omura S (1992) Transformation of vita- min D3 to 1 alpha,25-dihydroxyvitamin D3 via 25-hydroxyvitamin D3 using Amycolata sp. strains. Appl ... 7500 E-mail: tsakaki@pu-toyama.ac.jp Database Structural data are available in the Protein Data Bank database under the accession numbers 3CV8 and 3CV9 (Received 14 May 2010, revised 1 July 2...

Ngày tải lên: 15/03/2014, 23:20

... and 10. The pK a value of this acid–base transition is estimated based on the increase of absorbance at 418 nm as pH increases. Curve fitting of the fraction of thealkaline form to the calculated ... reductase was also examined. As illustrated in Fig. 8, the obtained spectra are clearly discriminated from those of the ascorbate- supported reaction. Although addition of 14 equiv...

Ngày tải lên: 23/03/2014, 20:22

... 0.81 ATcAAACA 0.03 c ATtAAACA 0.01 c ATGcAACA 0.69 ATGgAACA 0.20 c I ATGAgACA 0.02 c ATGAAgCA 0.05 c ATGAAAgA 0.30 III ATGAAACg 0.26 a PREs represented in the FUS1 promoter (Fig. 4B). b RCS for each oligo ... significant Table 1. RCS of mutant PREs for binding of wild-type Ste12 to a PRE consensus (ATGAAACA) in vitro. FUS1 PRE a Sequence RCS b II ATGAAACA 1.00 IV tTGAAACA 0.27 c...

Ngày tải lên: 06/03/2014, 22:21

... representation of the interpretation of logical metonymy and a more thorough evaluation method than that of Lapata and Lascarides (2003). By carrying out a human experiment we prove that such a representation ... followed by Lapata and Lascarides (2003) used text corpora to automatically derive interpretations of metonymic phrases. 1 Utiyama et al. (2000) used a statistica...

Ngày tải lên: 20/02/2014, 09:20

... that T4 DNA ligase binds ATP covalently forming a lysine (e-amino)-linked adenosine monophosphoramidate [8]. Harvey et al. [9] have demonstrated that sealing of the pre-adenylated nick in dsDNA ... with 80 mA current and lasted for 1–3 h. For each data point, the fluorescence of two chromatographically separated peaks of starting mater- ial and ligation product was obtained. Separati...

Ngày tải lên: 21/02/2014, 00:20

... 5¢-TAATATTGACcCCgGGCGCCAcAATCTCAAG-3¢ SmaI Y259Q 5¢-GCCATTTCCATAtTgaGTaCTGTTACCAAG-3¢ ScaI D266N 5¢-CATACTCAGCgTtaACTAAGCCATTTC-3¢ HpaI Y26 9A 5¢-TTGAGCCGCAgcCTCAGCgTCgAC TAAGCCATTTC-3¢ SalI Q272R 5¢-CTTAGGGATTAacGAGCCGCATACTCAGCgTCgAC ... GGTYGAYNGTSMATPHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ J 211 GGTYGAYNGTSMATTHVAGAAALILSKHPTWTNAQVRDRLESTATYLGNSFYYGKGLINVQAAAQ AMYL 211 GGTYGAY...

Ngày tải lên: 21/02/2014, 03:20

... conforma- tional changes in the fructose-1,6-bisphosphate and AMP domains. The representation of intrinsic fluorescence data as a ‘phase diagram’ reveals the existence of five intermediates, including ... [21]. Examination of protein unfolding by catalytic activity, size-exclusion high-performance liquid chromatography and 8-anilinonaphthalene- 1-sulfonate (ANS) binding Enzyme activ...

Ngày tải lên: 07/03/2014, 05:20

... nucleotide exchange on eukaryotic initiation factor 2. Nature 296, 93–95. 7 Sudhakar A, Krishnamoorthy T, Jain A, Chatterjee U, Hasnain SE, Kaufman RJ & Ramaiah KV (1999) Serine 48 in initiation factor ... F-12 lacking l-leucine (single amino acid starvation) or lacking l-glutamine, l-leu- cine, l-lysine and l-methionine (four amino acid starvation). Assay of effect of Nck-1 on eI...

Ngày tải lên: 07/03/2014, 05:20