Báo cáo khoa học: Kinetics of the quinone binding reaction at the QB site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes docx

... Biochem. 270) 4605 Kinetics of the quinone binding reaction at the Q B site of reaction centers from the purple bacteria Rhodobacter sphaeroides reconstituted in liposomes Francesco Milano 1 , ... that buries the quinone head in the inner part of the Q B pocket, thereby increasing the interaction energy between the ligand and the bi...

Ngày tải lên: 30/03/2014, 20:20

... properties of bound trappin-2, as this covalent linking may increase significantly the bioavailability of such an inhibitor at the site of in ammation, e.g. in the case of therapeutic administration, ... contribute to maintaining an in ammatory state and favor the persistence of microbial infections. Taken together, these observations suggest that increasing serine pr...

Ngày tải lên: 07/03/2014, 15:20

... may bind in the acyl- binding site of PA. Hydrolysis of p-NPA may thus proceed via reversed binding of the substrate, in which the leaving group, p-nitrophenol, occupies the acyl binding site ... kinetic constants for hydrolysis and synthesis of penicillin G, ampicillin and cephalexin. Furthermore, insight into the nature of the transition state in the...

Ngày tải lên: 08/03/2014, 08:20

... essentially independent of pH in the range 5.0–8.0 (not shown). Reduction of the rubredoxin domain of FlRd by flavorubredoxin reductase The isolated, genetically truncated rubredoxin domain (Rd-domain) of ... herein investigated by time-resolved spectroscopy working on the purified recombinant proteins. Results Reduction of flavorubredoxin reductase by NADH The kinetics...

Ngày tải lên: 16/03/2014, 11:20

... with the parameter estimates obtained in the absence of the inhibitors (see above). In the case of gallamine investigated in the presence of MeCN, the calculation of the kinetic parameters was based ... after incubating the enzyme–inhibitor mixture for 15 min at 25 °C. Uninhib- ited enzyme activity was determined by adding the corre- sponding solvent instead...

Ngày tải lên: 29/03/2014, 23:20

... the multiple cloning site of pUC18 at their N-terminal ends. The black bars in the GBd indicate the ‘A’ repeat [6,7]. Kinetics of Streptococcus sobrinus GTF-I reaction H. Komatsu et al. 532 FEBS ... in the intermediate oligosaccharides during the glucan syn- thesis can be interpreted with respect to the kinetic properties of enzymes. In other words, the rel...

Ngày tải lên: 14/02/2014, 22:20

... binding. Pairing of wild-type and point-mutated IGF-II binding sites between two dimerized mini-receptors had no effect on the function of the contralateral binding site, indicating IGF-II binding ... Man-6-P binding or whether the mutated binding pocket becomes distorted, preventing Man-6-P binding. Glucose 6-phosphate (Glc-6-P) is unable to bind to the two Man-6-P...

Ngày tải lên: 18/02/2014, 08:20

... investigated the metal -binding reactivity of the isolated a domain of human metallothionein isoform 1a, with specific emphasis on resolving the debate concerning the cooperative nature of the metal -binding ... example of the complexity introduced by the presence of the b domain in efforts to elucidate the potentially cooper- ative nature of the metal -bind...

Ngày tải lên: 19/02/2014, 00:20

... form part of the copper binding sites in this protein. Although there are four histidines in the C-terminal, another histidine is present at position 18, and given the folded state of the protein it ... among the three human cystatins. Further support for the premise that the C-terminus could be the copper binding domain comes from stud- ies of the P79S mut...

Ngày tải lên: 19/02/2014, 06:20

... c-N-methylam- inobutyrate, a compound generated during the catabolism of nicotine. The identification of this enzyme reveals, for the first time, the metabolic fate of the pyrrolidine ring of nicotine during ... family may indicate that this is the site of attachment of tetra- hydrofolate to the enzyme. c-Aminobutyrate produced during the reaction may enter the...

Ngày tải lên: 19/02/2014, 16:20