Báo cáo khoa học: Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus pot

... Interactions of the peripheral subunit-binding domain of the dihydrolipoyl acetyltransferase component in the assembly of the pyruvate dehydrogenase multienzyme complex of Bacillus stearothermophilus Hyo-Il ... Competitive interaction of component enzymes with the peripheral subunit- binding domain of the pyruvate dehydrogenase...

Ngày tải lên: 30/03/2014, 20:20

... so the nitro- genous group of agmatine must be within 3 A ˚ of N5 of the isoalloxazine ring of the flavin. Agmatine, however, binds poorly to MAO-A, giving only 50% inhibition of the oxidation of ... and the resulting inhibition. The use of purified enzyme simplifies the system for kinetics and also allows observation of the spectrum of the flavin cofactor in...

Ngày tải lên: 07/03/2014, 10:20

... protein; GST, glutathione S-transferase; HD, Huntington’s disease; HIP1, Huntingtin interacting protein 1; HIPPI, Huntingtin interacting protein 1 protein interactor; Htt, Huntingtin; IOD, integrated ... decrease in the intrinsic fluorescence of GST–HIPPI protein, indica- ting binding of the protein with this mutated motif (Fig. 1B, panel II). The apparent dissociation constant (K...

Ngày tải lên: 07/03/2014, 10:20

... which is a precursor of hypusine in the eIF-5A protein [32]. The model in Fig. 5, in which the orientation of the spermidine residue of EF-P is facing the mRNA-binding pocket of the 30S subunit is ... important to the function of the EF-P protein is the fact that the interactions of the molecule are in close proximity to the peptidyltransferase ce...

Ngày tải lên: 16/03/2014, 06:20

... receptors, including human RXR (which is the ortholog of USP) have shown that an area on the surface of the ligand binding domain involving the C-terminus of a-helix 3, a-helix 4, and the N-terminus of ... binding by either purified recombinant dUSP, or by components of Sf9 nuclear extracts. Binding conditions for recombinant dUSP were 1.0 lg of dUSP, 1 lg dIdC, 3 lLof5...

Ngày tải lên: 23/03/2014, 13:20

... calori- metry results using magainin II with those of the more potent magainin analog, [Ala8,13,18]-magainin II amide [30]. We find that the peptide-induced changes in the transition of DPam 2 PtdEtn are ... hydration of the headgroup and the higher energy cost of bending the membrane. In addition, the presence of the anionic lipid PtdGro in these mixtures is im...

Ngày tải lên: 31/03/2014, 07:20

... protein H appear to be involved in the interaction as the binding of 125 I-labelled DS was reduced to isogenic derivatives of the AP1 strain lacking either of these surface proteins (Fig. 2). The ... charged sulfate groups of the GAG chains and positively charged regions of the ligand. Typi- cally, the heparin-binding domains of known GAG-binding proteins are rich...

Ngày tải lên: 20/02/2014, 11:20

... pro- cessed into fragments by proteolysis. Recently, the Brichos domain has been found also in other proteins, including a protein (TFIZ1) that binds trefoil domains [13]. The Brichos domain may be involved ... part of proSP-C (CTC)]. CTC contains a  100-residue Brichos domain, cov- ering the region from residue 94 to the C-terminal end. The name Brichos refers to the fa...

Ngày tải lên: 07/03/2014, 05:20

... 5.1-fold, respectively. Increased protein level of TFAM induced by D-Gal To further understand the expression of TFAM protein in the inner ear, western blot analysis was performed. The protein levels of TFAM in the ... in mutant mtDNA harboring the com- mon deletion. By applying the specific probe, we can even detect the presence of the common deletion in the...

Ngày tải lên: 14/03/2014, 23:20

... within the mature 18S rRNA (Fig. 7C, asterisk). These results further indicate the involvement of Utp25p in the early steps of processing of pre-40S. To determine whether Utp25p might associate in ... further indicating that this protein is associated only with the early pre- rRNAs. This is in accordance with the hypothesis of the involvement of Utp25p in the e...

Ngày tải lên: 22/03/2014, 21:21