Báo cáo khoa học: Disruption of transport activity in a D93H mutant thiamine transporter 1, from a Rogers Syndrome family pdf

... Disruption of transport activity in a D93H mutant thiamine transporter 1, from a Rogers Syndrome family Dana Baron 1 , Yehuda G. Assaraf 2 , Stavit Drori 2 and Ami Aronheim 1 1 Department of ... with the D93H mutant THTR1 and treated with tunicamycin displayed a similar lack of plasma membrane localization of the mutant transporter as well as a...

Ngày tải lên: 30/03/2014, 20:20

... orthovanadate, calyculin A, cyclosporine, and okadaic acid, was stud- ied. Insensitivity to okadaic acid, a potent inhibitor of the PP 2A and PP2B family of phosphatases, is one of the unique characteristics ... compilation ª 2008 FEBS Loss of kinase activity in Mycobacterium tuberculosis multidomain protein Rv1364c Preeti Sachdeva 1,2 , Azeet Narayan 1 , Richa Misra 1 , V...

Ngày tải lên: 30/03/2014, 02:20

... below. Instability of the mutant enzyme Further analysis of the kinetic parameters of the G167E mutant was hindered by rapid inhibition of the activity of the bc 1 complex during the assay, which was particularly noticeable ... the activity in samples from the patient. This was in agreement with the data obtained in this study, using the mutant yeast enzyme. It has...

Ngày tải lên: 07/03/2014, 15:20

... Germany). The radioactivity was quantified in a Canberra-Packard Tricarb-2500 counter. The kinetic constants of transport were estimated using the method of Hanes. All data represent means of at least three ... but recent analysis demonstrated that UhpC from E. coli can act not only as a sensor but also as a carrier that facilitates a Glc6P/P i antiport mode of transport...

Ngày tải lên: 08/03/2014, 08:20

... 5¢-TGCTCTCCTAC TCCTGATGAAG-3¢; reverse, 5¢-GGGCATGTGGTTCCT GTCC-3¢. Cdc20: forward, 5¢-GCAACAGGAGGAGGA ACCAG-3¢; reverse, 5¢-CATCCACAGCACTCAGACAG G-3¢. Cdkn 1a: forward, 5¢-AAAGTGTGCCGTTGTCTC-3¢ ; reverse, 5¢-AAAGTTCCACCGTTCTCG-3¢. ... 5¢-AAAGTTCCACCGTTCTCG-3¢. Accn5: for- ward, 5¢-GGTGACCATCCGCCAACTG-3¢; reverse, 5¢-CC GTAAGTGCTGTAGGTAATGAAG-3¢. Onecut1: forward, 5¢-CCTCTATGAATAACCTCTATACC-3...

Ngày tải lên: 23/03/2014, 06:20

... chromatography as des- cribed previously [28]. Antiserum against bovine a2 M was purchased from Yagai Research Center (Yamagata, Japan). All other chemicals were of reagent grade and were purchased from various ... hydrolyzing activity of cathepsin E and cathepsin D To assess the inhibitory capacity of a2 M from various sources for cathepsin D and cathepsin E, we first purified...

Ngày tải lên: 23/03/2014, 21:20

... expression and activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinflammatory cytokines, leading to a loss of catalytic activity. This downregulation was at ... significantly at 24 h after CLP and this increase was attenuated by GdCl 3 . The malondialdehyde (MDA) level in CLP rats was 1.8-fold that of sham-operated rats. The incr...

Ngày tải lên: 14/02/2014, 18:20

... Horse- radish peroxidase-conjugated antibody against caspase-3 (#610325) was from BD Pharmigen, and antibody against b-actin (A5 441) was from Sigma. Statistical analysis All data are expressed as ... As shown in Fig. 4A, increased phosphorylation of eukaryotic translation initiation factor 2a (eIF 2a) was apparent after 16 h of stearate treatment, reached a peak at 22 h, and dec...

Ngày tải lên: 14/02/2014, 22:20

... forward 5¢-GTAGGCATGAGAACGGGA AG-3 and for reverse 5¢-GGGGGTAAGAGGAGGAGA AA-3¢ and for CERK-negative forward 5¢-CCGCAAG AGGCTTTATTGTC-3 and reverse 5¢-TATGCCAAGGA CACGGAGAT-3¢, as a negative control ... MEBCYTO Apoptosis Kit was purchased from Medical and Biological Laboratories (Nagoya, Japan), and a Nuclear Extract Kit was from Active Motif (Carlsbad, CA, USA). The ChIP Assay Kit wa...

Ngày tải lên: 18/02/2014, 18:20

... El-Agnaf OM & Allsop D (2002) Formation of hydrogen peroxide and hydroxyl radicals from A( beta) and alpha-synuclein as a possible mechanism of cell death in Alzheimer’s disease and Par- kinson’s ... FEBS downstream of the initiator ATG in exon 1 (Ex1) of the 67 exon-containing htt gene [5]. Pathogenesis in HD correlates with the cleavage of mutated htt and the releas...

Ngày tải lên: 19/02/2014, 06:20