Báo cáo khoa học: Structural aspects and biological properties of the cathelicidin PMAP-36 pot

... Structural aspects and biological properties of the cathelicidin PMAP-36 Marco Scocchi, Igor Zelezetsky, Monica Benincasa, Renato Gennaro, Andrea Mazzoli and Alessandro TossiDepartment of ... other cathelicidins such as SMAP-29,BMAP-27 and BMAP-28. The amidated form wouldfurther increase the charge of the dimer to +28 (seeTable 1).Here we further investigated the expression of ... the pro-form level of the cathelicidin [21], and this structure islikely to persist in the mature peptide. Indeed, it mayexplain the different activity spectra and potenciesobserved for the...

Báo cáo khoa học: Mechanistic aspects and redox properties of hyperthermophilic L-proline dehydrogenase from Pyrococcus furiosus related to dimethylglycine dehydrogenase⁄oxidase potx

... of heat-denatured PRODH showing the [M +H]+ion of FAD cofactor with an m ⁄ z peak of 787 identical to that of the authentic FAD standard. The m ⁄ z peak at 825 repre-sents the K+adduct of ... 10ðpHÀpKa2Þð4Þwhere EH and E are the catalytic activities of the proto-nated and unprotonated forms of the ionization group,respectively, and Tmaxis the theoretical maximal value of kcat⁄ Km.Product ... midpoint potential forPRODH of ) 407 ± 5 mV. Thermodynamic para-meters for the reduction of PRODH by sodium dithi-onite were calculated from the temperature dependence of the midpoint potential, and...

Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt

... with the higher inhibitory activity of the cEL peptide and the verylow inhibitory activity of the cYT peptide compared toother peptides. The ﬂanking residue of the b-turn, i.e. Tyr3 of the cVYpeptide ... [38]. Analysis of the crystal structure of CD2 revealed that on either side of t he binding surface of CD2, there are b-turns whic h stabilize the b-strands. Thus,we hypothesized that these b-turns ... suggestive of a b-turn in the peptide and the proximity of amide protons due to compact nature of the structure. However, t he coupling constant of most of the amide protons was in the range of 6–8...

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... transition and lipidheadgroup conformations The determination of the b«a gel-to-liquid crystallinephase transition of the acyl chains from the evaluation of the symmetric stretching vibration of the ... answer the questionwhether the cubic structure observed is of the ÔnormalÕ(right side out) or the inverted type, the geometry of the molecule with its bulky headgroup is in favor of the formertype. ... todetermine the phase behavior via the analysis of the peak position of the symmetric stretching vibration of the methylene groups. Additionally, this technique wasapplied to monitor the conformation of...

Báo cáo khoa học: "Formal aspects and parsing issues of dependency theory Vincenzo Lombardo and Leonardo Lesmo" potx

... follow the events; the separator symbol I helps to keep trace of the sets in the stack; finally, we have left in plain text the d-quad sequence of the dotted rules; the other components of the ... the complexity count. T-stack has a number of elements which depends on the maximum length of the chain of predictions. Since the number of rules is O(n), the size of the stack is O(n). The ... given the grammar G1. 789 from the grammar. The selection is carried out by matching the head of the rules with the words of the sentence. The second step follows Earley's phases on the...

Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf

... evidencesupporting a structural interruption of the geometry of the recognition helix for either Antp or the TTF-1HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal ... consistent with the NOE trend of the correspondingadjacent residues and hence does not conﬂict with the global interpretation of the data. With the exclusion of the N-terminal octapeptidyl and C-terminal ... mobility, and is indicative of the degree of internal restraint of each15N–1H vectormotion. In Fig. 4, most of the points cluster in the same region. The dashed curve, called the theoreticalTable...

Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc

... 2008 The Authors Journal compilation ª 2008 FEBS 4391Zn-binding motif and the two disulﬁde bonds respec-tively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the ... of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu-bating the protein at 60, 70 and 80 °C for 30 min and thenrecording the CD spectra of the incubated samples ... analyses of the predictedamino acid sequence showed that the C-terminal half of the RepA of the pIT3 plasmid is sequence-similar to the helicases of the phage-encoded superfamily III pro-teins. The...

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... lysozyme. This hypothesis is not con-ﬁrmed by the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiCstructures.Despite the deletion of the B-loop, the two putativecatalytic ... equilibrium. The top panel represents a standardmixture of GlcNAc oligomers showing the standard 60 : 40 ratiobetween the a-anomer and the b-anomer at equilibrium. The otherpanels show the results of ... N-terminalextension of eight and seven residues respectively(Fig. 1). The N-termini of the plant enzymes and the ﬁrst residue in the ChiG structure (Phe12) (Fig. 4A),are located on the opposite side of the...

Báo cáo khoa học: GTP binding and hydrolysis kinetics of human septin 2 pot

... after the completion of most of the studies describedherein. Given the conserved nature of this residue we there-fore mutated the residue back to arginine and found thatnone of the properties of ... with the ratio of the concentration of bound GTPcS over the concentration of SEPT2 divided by the freeGTPcS ([b] ⁄ [SEPT2] ⁄ [f] – y axis) plotted against the ratio of boundGTPcS over the SEPT2 ... the ﬁrst detailed kineticstudy of the GTP binding and hydrolysis properties of a single septin protein and demonstrate that phos-phorylation of serine 218 by casein kinase II altersthese properties. ...

Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx

... further our knowledge withrespect to the mechanisms of action of these proteins,it is crucial that we deﬁne the precise boundaries of the STI1-homologous domain and compare the struc-tures of ... structure. The quality of the struc-tures was assessed using the reﬁned energy terms and the procheck program [37]. The surface electrostatic potentialdistribution of the best overall model of XPCB–hHR23Bwas ... nmrpipe[32] and analyzed using sparky [33]. The errors of R1 and R2were estimated from the errors in the single exponentialdecay ﬁtting, and those of the 15N-1H NOEs were obtainedfrom the difference...

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