Báo cáo khoa học: Structural aspects and biological properties of the cathelicidin PMAP-36 pot

... Structural aspects and biological properties of the cathelicidin PMAP-36 Marco Scocchi, Igor Zelezetsky, Monica Benincasa, Renato Gennaro, Andrea Mazzoli and Alessandro Tossi Department of ... other cathelicidins such as SMAP-29, BMAP-27 and BMAP-28. The amidated form would further increase the charge of the dimer to +28 (see Table 1). Here we further investiga...

Ngày tải lên: 30/03/2014, 20:20

... of heat-denatured PRODH showing the [M +H] + ion of FAD cofactor with an m ⁄ z peak of 787 identical to that of the authentic FAD standard. The m ⁄ z peak at 825 repre- sents the K + adduct of ... 10 ðpHÀpK a2 Þ ð4Þ where EH and E are the catalytic activities of the proto- nated and unprotonated forms of the ionization group, respectively, and T max is the...

Ngày tải lên: 30/03/2014, 08:20

... with the higher inhibitory activity of the cEL peptide and the very low inhibitory activity of the cYT peptide compared to other peptides. The flanking residue of the b-turn, i.e. Tyr3 of the cVY peptide ... [38]. Analysis of the crystal structure of CD2 revealed that on either side of t he binding surface of CD2, there are b-turns whic h stabilize the b-str...

Ngày tải lên: 19/02/2014, 13:20

... transition and lipid headgroup conformations The determination of the b«a gel-to-liquid crystalline phase transition of the acyl chains from the evaluation of the symmetric stretching vibration of the ... answer the question whether the cubic structure observed is of the ÔnormalÕ (right side out) or the inverted type, the geometry of the molecule with its...

Ngày tải lên: 21/02/2014, 00:20

... follow the events; the separator symbol I helps to keep trace of the sets in the stack; finally, we have left in plain text the d-quad sequence of the dotted rules; the other components of the ... the complexity count. T-stack has a number of elements which depends on the maximum length of the chain of predictions. Since the number of rules is O(n),...

Ngày tải lên: 17/03/2014, 07:20

... evidence supporting a structural interruption of the geometry of the recognition helix for either Antp or the TTF-1 HD, the anomalous amide exchange pattern and the NOE connectivity data of the C-terminal ... consistent with the NOE trend of the corresponding adjacent residues and hence does not conflict with the global interpretation of the data. With the...

Ngày tải lên: 18/02/2014, 16:20

... 2008 The Authors Journal compilation ª 2008 FEBS 4391 Zn-binding motif and the two disulfide bonds respec- tively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the ... of 0.2 nm, and a bandwidth of 1 nm. Thermal stability of Rep245 was measured by incu- bating the protein at 60, 70 and 80 °C for 30 min and then recording the C...

Ngày tải lên: 18/02/2014, 18:20

... lysozyme. This hypothesis is not con- firmed by the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiC structures. Despite the deletion of the B-loop, the two putative catalytic ... equilibrium. The top panel represents a standard mixture of GlcNAc oligomers showing the standard 60 : 40 ratio between the a-anomer and the...

Ngày tải lên: 07/03/2014, 11:20

... after the completion of most of the studies described herein. Given the conserved nature of this residue we there- fore mutated the residue back to arginine and found that none of the properties of ... with the ratio of the concentration of bound GTPcS over the concentration of SEPT2 divided by the free GTPcS ([b] ⁄ [SEPT2] ⁄ [f] – y axis) plotted against...

Ngày tải lên: 07/03/2014, 12:20

... further our knowledge with respect to the mechanisms of action of these proteins, it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the struc- tures of ... structure. The quality of the struc- tures was assessed using the refined energy terms and the procheck program [37]. The surface electrostatic potential distributio...

Ngày tải lên: 07/03/2014, 17:20