Báo cáo khoa học: Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx
Báo cáo khoa học: Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx
... comparing the phosphorylation of the
natural substrates of the double mutant with the single
mutant, it seems that the mutation N45D in the
double mutant counteracts the negative effect(s) of the
N64D ... bound
to the 3¢-OH group of the substrate while there is no
density for that interaction in the mutant structure
(Fig. 2B).
Structural basis f...
Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx
... different
substrate- binding mode results from conformational
control of the enzyme on the side chain rotamer of the
bound substrate. For example, in AsnO, a trans con-
former is selected for the v
1
torsion ... study, we investigated the substrate specificity
of the nonheme iron oxygenase VioC and the kinetic
parameters for the hydroxylation reaction of th...
Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt
... orientation of the side chain
of Glu221. The second conformation of the
side chain of Glu221, which also adopts two
conformations but not correlated with the
anomeric form of the bound mannose is
shown ... [34]. Therefore, we conclude that the
reason for the difference in energetics of the )1 subsite
between PAL and con A must be due to details in the
interacti...
Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A docx
... between the carboxylate
group of E422 and the amine groups of K43 and K44
of monomer B, and the side chains of R421 and D12
of monomer A. These residues point towards the inte-
rior of the peptide ... area.
Figure 4A shows the 20 lowest-energy conformers
from the selected cluster modelled using the Haddock
docking calculations. The rmsd values for the 20
lowe...
Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt
... half-buried, and the form of the cleft is markedly
different from those of TvuCMBP and EcoMBP.
These observations suggest that the structure of the
N-domain part and the capacity of the sugar-binding
site ... complex,
the specificity of the bacterial sugar-binding proteins
should be defined in terms not of the affinities for
sugars but of whether the protein...
Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot
... hybridize to the 5¢-end
of the loops and propagate to nucleotides involved in
the formation of the helix. The strong preference for
the 5 ¢-side of the loops in comparison with the 3 ¢ -side
may ... suggest that these guanine bases are crucial for the creation of
Co
2+
binding sites and that they appear to be involved in the coordination
of the ion to the...
Báo cáo Y học: Structural basis for the inhibitory efficacy of efavirenz (DMP-266), MSC194 and PNU142721 towards the HIV-1 RT K103N mutant doc
... reduced by
the s tacking of O d1 in between the plane of the thiourea
moiety and the Ca of G190.
Hence, the undefined orientation of the N103 amide Od1
and Nd2 atoms may r eflect the repulsive forces ... are
superimposed in the mutant NNIBP. The cyclopropyl-
group of MSC194 and the methyl group of PNU142721
partly overlap the trifluoromethyl group of Efavire...
Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx
... char-
acter of the substrate, this Xyl becomes the product of
hydrolysis or the nonreducing end of the leaving
group. A stereo view of the mode of binding of
MeGlcA
2
Xyl
3
is shown in Fig. 5A. The interactions ... three-dimensional
structure of the E. chrysanthemi GH30 enzyme [16],
together with its established mode of action [14],
allowed us to present a hypot...
Báo cáo khoa học: Structural basis for stereo-specific catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx
... binding of
coenzyme and substrate to the enzyme in its open con-
formation followed by the closure of the interdomain
cleft. In FDH, the structural ordering of the C-ter-
minal helix and the rotation ... a
resolution of 1.98 A
˚
. The structural analysis of HGDH
together with the comparison with other structurally
characterized members of the
D
-2-hydroxyac...
Báo cáo khoa học: Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2) potx
... demonstrated the transport
of conjugated and unconjugated lipophilic anions by
MRP2. The absence of MRP2 from the canalicular
membrane of human hepatocytes is the molecular basis of
the Dubin–Johnson ... amino-acid identity of only 48% between the laterally
localized isoform MRP1 a nd the a pically localized isoform
MRP2 [1] hampers the identification of apical so...