Báo cáo khoa học: Nuclear import of mPER3 in Xenopus oocytes and HeLa cells requires complex formation with mPER1 pdf

... Nuclear import of murine PER and CRY proteins in Xenopus oocytes. mPER1 and mPER2, but not mPER3, are phosphorylated and imported into the nucleus of Xenopus oocytes. (A) 35 S-labelled mPER1, ... the C-terminal region of mPER1. Complex formation with mPER1 promotes nuclear import of mPER3 in Xenopus oocytes As heterodimerization of clock pr...

Ngày tải lên: 30/03/2014, 20:20

... decreases in intracellular spermine pools and increased spermidine levels in SMO1 and SMO5 cells. These changes in spermine and spermidine levels in both SMO1 and SMO5 overexpressing cell lines were ... microscopy of immunofluorescent staining of SMO in cells overex- pressing each of the isoforms provided direct visuali- zation and confirmation of protein localiza...

Ngày tải lên: 07/03/2014, 05:20

... upon incubation with increasing concentrations of single polyamines, starting from a concentration of 1 lm polyamines, which is comparable to the concentration of polyamines forming NAPs in the ... associated with a reduction in the overstretching forces of the DNA strands [22,23], (b) that the DNA melting tempera- ture is directly correlated with the chain length of inter...

Ngày tải lên: 23/03/2014, 15:20

... B and A chains of insulin [1] and has an important structural role in the proper folding and disulfide bonding in insulin [2]. After proinsulin cleavage, it is released to the blood together with ... process. Early studies on proinsulin and insulin behavior in solution revealed self-associating properties [19–21] and, as a result, insulin is found to form zinc-induced hexame...

Ngày tải lên: 18/02/2014, 04:20

... is conserved in all subunit types (Hc1 and Hc2) with the exception of PmaHc1. An insertion of nine amino acids in PamHc2 starting at amino acid 435 is unique to subunit types 2. On the amino acid ... coordinated by three histidines in two distinct binding sites. Some hemocyanins assemble into large oligomers of up to 8 · 6 subunits [1]. The occurrence and properties of hem...

Ngày tải lên: 18/02/2014, 08:20

... to prevent the entry of bacteria into the epithelial layer and their invasion into the lower parts of the gastro- intestinal tract [7]. Their finding of buforin-I in porcine, bovine and human stomach ... membrane and binding to the DNA without inducing cell lysis. In the present study, HNr and H4-(86–100), like the quinolone antibiotic ciprofloxacin, displayed rapid (within 30...

Ngày tải lên: 18/02/2014, 14:20

... Localization of silicatein in spicules and in intra- and extracellular vesicles by TEM immunogold labeling. (i) Association of silicatein with spicules. (A) Strong antibody reactions are seen within the ... bonds (red). In the cata- lytic center of silicatein, the serine (Ser) oxygen makes a nucleo- philic attack on the silicon, resulting in displacement of p-aminophenol...

Ngày tải lên: 18/02/2014, 16:20

... 2008) doi:10.1111/j.1742-4658.2008.06522.x The folding of eukaryotic DNA into the 30 nm fibre comprises the first level of transcriptionally dormant chromatin. Understanding its structure and the processes of its folding and unfolding is ... or of nucleosomes in the fibre [5,19]. It is a cleavable crosslinker with two succinimidyl groups, which react with lysines independently....

Ngày tải lên: 18/02/2014, 18:20

... Treatment with Quercetin did not inhibit ROS generation, and enhanced cytotoxicity of Topotecan in both cells. In contrast, NAC enhanced resistance against doxorubicine and vincristine in MRP1 ... T-helper cells, and 50% of the young Ung–/– mice investigated have no detectable NK/NKT-cell population in their spleen. In addition, production of the cytokines interferon...

Ngày tải lên: 19/02/2014, 07:20

... phosphorylation of Tyr304 in the cytoplasmic tail of ephrin B2 confers high-affinity and bifunctional binding to both the SH2 domain of Grb4 and the PDZ domain of the PDZ-RGS3 protein Zhengding Su, Ping Xu and ... effect of tyrosine phosphorylations on the binding affinity of ephrinB2(301–333) to the SH2 domain of Grb4 and to the PDZ domain of PDZ-RGS3. In the combinat...

Ngày tải lên: 19/02/2014, 12:20