... aDNA fragment containing the Gmlbc3 promoter, the gusA gene, and the pAnos termination sequence bydigestion with HindIII; the digestion was followed by self-ligation of the remaining vector to ... responsiblefor intestinal absorption of vitamin B12facilitating itsinternalization [2,3,5]. Lack or malfunction of this Cblbinder hampers the uptake of the minute amounts of the vitamin present in food. ... periodical shaking.Then, the suspension was placed on ice for 1 h, whichstabilizes the thermo-labile bond between the cobalt atom ofCbl and the amino group. At that point the matrix can beeither used...