Báo cáo khoa học: V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) system: enhancement by azide ppt

... into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp Vasthi ... about 5% of the arginase activity of the wild- type enzyme. As measured by k cat ⁄ K m , the catalytic efficiency of the Asn149Asp variant was found to be about 36-fold lo...

Ngày tải lên: 20/02/2014, 02:21

... maintenance of the axis of polarity. The possible molecular mechanisms responsible for this asymmetry are discussed. Abstracts 557 V1–Varia V1-001P Hemolysis of human red blood cells by riboflavin-Cu(II) ... presence of Cu(II) generates reactive oxygen species (ROS) which can hemolyse human red blood cells (RBC). In the present work we examined the effect of s...

Ngày tải lên: 30/03/2014, 20:20

... presence of high levels of GSH (data not shown), suggesting that an oxidoreductase, such as a glutaredoxin, might be required for reduction of the mixed disulfide. In this hypothesis, glutaredoxins would ... under stress would allow redistribution of the reducing power in the chloroplast by decreasing NADPH consumption and thereby increasing electron availability at the redu- cing si...

Ngày tải lên: 19/02/2014, 05:20

... this case, the lack of binding to the reduced TC is caused rather by loss of the correct three-dimen- sional organization than by disruption of the S-S bond, per se. The effect of heparin on interaction ... unfractionated heparin at a concentration of 100 unitsÆmL )1 . Fig. 2. Binding of anti- (human TC) sera to reduced and unreduced TC in a western blot. Recombinant human...

Ngày tải lên: 20/02/2014, 01:20

... finite-state models of language are crucially limited in their explanatory power by the local- ity of the probability distribution and the narrow scope of information used by the model. We show ... probability. Under this general assumption, the overall diffi- culty of a sentence is expected to be measured or predicted by the mean size of probability decrease. That is, probabili...

Ngày tải lên: 20/02/2014, 11:21

... location of the ceramide tail of GM2 as identified by crystallo- graphic analysis of lipid complexes of GM2AP [21], which positions the tetrasaccharide head-group of the substrate in direct vicinity of ... appropriate amount of HexA were incubated at 37 °C for 30 min in the absence or presence of various amounts of GM2AP (up to 10 lg). Reactions were stopped by the additi...

Ngày tải lên: 07/03/2014, 12:20

... T-cell subsets are expanded by interaction with SSA. Some authors indicated clonal expansion of T -cells bearing human Vb3, Vb12, Vb17 and Vb19 [20]. Others showed proliferation of human T -cells bearing Vb1, ... SSA have affinity for human Vb5.2 and V b1withK d of 9–11 l M with a fast k ass and a moderately fast k diss . In spite of the reported stimu- lation of V b2.1 bear...

Ngày tải lên: 07/03/2014, 16:20

... view of selected residues of hamster NAT2. Residues are colored by atom type: carbon, nitrogen, sulfur and oxygen in white, blue, orange and red, respectively. X. Zhou et al. The catalytic role of ... perturbation of the active site by complete removal of the side chain mainly affected enzyme acetylation (k 2 ). pH dependence of NAT acetylation Usually, the pH dependence of...

Ngày tải lên: 16/03/2014, 00:20

... involved affinity purification of profilin- actin complexes on poly(l-proline) Sepharose, enrichment of actin by a cycle of polymerization and depolymerization, isoform separation by hydroxyapatite chromatography, ... mutant of M90T cured of the large virulence plasmid. Shigella strains SC300 and SC301 contain plasmid pIL22 encoding an afimbrial adhesin of uropathogenic E. coli [5...

Ngày tải lên: 16/03/2014, 06:20

... confirm that addition of 10 mm sodium butyrate does not alter the polar- ized membrane properties of MDCK cells (Fig. S1). Cells were kept on ice for the duration of the experiment. Cells were rinsed ... oculodentodigital dysplasia (ODDD), a rare human developmental disorder characterized by defects in the craniofacial bones, loss of tooth enamel, and abnormal soft tissue sep...

Ngày tải lên: 23/03/2014, 04:20