Báo cáo khoa học: L1–Protein Diagnostics, Protein Determination ppt

... strong antifungal agent, our studies with partially purified lipopeptides Abstracts 508 L1 Protein Diagnostics, Protein Determination L1-001 Reference measurement procedures and external quality assessment ... various exoproteins of different strains depends on bacterial growth conditions. The aim of this work was the description of the exoprotein patterns of the different strains stu...

Ngày tải lên: 30/03/2014, 20:20

... structure determination has provided a plethora of protein structures, but not as many structures of nucleic acids or of their complexes with proteins. The recognition of DNA and RNA molecules by proteins ... full-length protein can be extremely rewarding, as shown by the information obtained with the tumor suppressor protein BRCA2 and its interac- tion with DNA [44,45]. As compared w...

Ngày tải lên: 14/02/2014, 19:20

... green fluorescent protein; EYFP, enhanced yellow fluorescent protein; FACS, fluorescence- activated cell sorting; GFP, green fluorescent protein; GRASP, Golgi reassembly stacking protein; GRASP55F, ... comprising an important sequence for the interaction with the PDZ proteins: Mint-3, AMPA binding protein and gluta- mate receptor interacting protein (GRIP). It would therefore be interesti...

Ngày tải lên: 18/02/2014, 04:20

... dominant-negative Ga i protein constructs [11]. G proteins are located down- stream of G -protein- coupled receptors (GPCR) [12]. GPCR represent a large family of cell-surface proteins mediating the ... heterotrimeric form [15]. Regulators of G -protein signalling (RGS) interact directly with the G protein a subunit in order to inhi- bit G -protein signalling [16]. RGS proteins belong t...

Ngày tải lên: 18/02/2014, 13:20

... De novo design of the hydrophobic cores of proteins. Protein Sci. 4, 2006–2018. 80. Dahiyat, B.I. & Mayo, S.L. (1996) Protein design automation. Protein Sci. 5, 895–903. 81. Looger, L.L. & ... what makes a protein Ônative-likeÕ? In essence, a native-like protein is one with thermodynamic and structural properties that are exhibited by ÔnormalÕ cellular proteins (i.e. native...

Ngày tải lên: 19/02/2014, 12:20

... HRP, horseradish peroxidase; MAP, mitogen-activated protein; MEK, mitogen-activated protein ⁄ extracellular signal-regulated kinase kinase; PKA, protein kinase A; Sos1, son of sevenless 1. 2304 ... Ras family of small G proteins. The activity of Ras proteins is under tight control of several classes of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins. Mammalian S...

Ngày tải lên: 19/02/2014, 18:20

... initiated when adaptor proteins are recruited to the IRSs through their src homology 2 domains (a) the growth factor receptor binding protein activates the ras/ mitogen-activated protein kinase pathway ... 272, 30075–30082. 13. Walaas, O., Horn, R.S. & Walaas, S.I. (1997) The protein kinase C pseudosubstrate peptide (PKC19-36) inhibits insulin-stimulated protein kinase activity an...

Ngày tải lên: 20/02/2014, 02:21

... indicated. Ubiquitinated proteins were extracted on cobalt beads and immu- noblotted with anti-HA IgG. The expression of transfected proteins was monitored on total protein extracts by immunoblotting ... Cell Endocrinol 270, 23–32. 10 Chen J & Archer TK (2005) Regulating SWI ⁄ SNF subunit levels via protein- protein interactions and prote- asomal degradation: BAF155 and BAF170 limit ex...

Ngày tải lên: 06/03/2014, 09:22

... [8,9]. Localization of different proteins within living cells became possible with the fusion of a fluorescent protein, e.g. green fluorescent protein, to the protein of interest [10]. Fluorescent protein domains ... affinity-purifying proteins using a bis-arsenical fluorescein. Protein Sci 9, 213–217. 20 Griffin BA, Adams SR, Jones J & Tsien RY (2000) Fluorescent labeling of recombin...

Ngày tải lên: 06/03/2014, 11:20

... M (2003) Chromosomal protein HMGN1 enhances the rate of DNA repair in chromatin. EMBO J 22 , 1665–1675. 15 Bustin M (2001) Chromatin unfolding and activation by HMGN(*) chromosomal proteins. Trends ... motifs of the high-mobility-group chromosomal proteins. Mol Cell Biol 19, 5237–5246. 17 Bustin M & Reeves R (1996) High-mobility-group chro- mosomal proteins: architectural components tha...

Ngày tải lên: 07/03/2014, 00:20