Báo cáo khoa học: Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide–metal ion complexes pptx
Báo cáo khoa học: Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide–metal ion complexes pptx
... FEBS Autophosphorylation of Archaeoglobus fulgidus Rio2 and crystal structures of its nucleotide–metal ion complexes Nicole LaRonde-LeBlanc 1 , Tad Guszczynski 2 , Terry Copeland 2 and Alexander Wlodawer 1 1 ... further information about the nature of the active site of Rio2 kinase and suggest a mechanism of regulation of its enzymatic activity. Abbre...
Ngày tải lên: 30/03/2014, 16:20
Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx
... HylP2 was crystallized and the crystals were soaked in two separate reservoir solutions containing ascorbic acid and lactose, respectively. The crystal structures of native HylP2 and its two complexes ... ligand. The crystals of the native protein were soaked in solutions containing ascorbic acid and lactose separately for 48 h and the crystal structures of the two...
Ngày tải lên: 23/03/2014, 04:21
Tài liệu Báo cáo khoa học: TEC family kinases in health and disease – loss-of-function of BTK and ITK and the gain-of-function fusions ITK–SYK and BTK–SYK pptx
... Authors Journal compilation ª 2011 FEBS MINIREVIEW TEC family kinases in health and disease – loss -of- function of BTK and ITK and the gain -of- function fusions ITK–SYK and BTK–SYK Alamdar Hussain 1,2, *, ... transcription of the ITK gene and is key for the productive infection of HIV in these cells. Accord- ingly, the inhibition of ITK activity compromises HIV infection,...
Ngày tải lên: 14/02/2014, 18:20
Tài liệu Báo cáo khoa học: Competition between innate multidrug resistance and intracellular binding of rhodamine dyes pdf
... continuously with an excitation wavelength of 555 nm and an emission wavelength of 575 nm. Inhibition of the NBD-Cl-sensitive efflux allowed the uptake of TMRM and its quenching within the cells. ... to our expectations, under these conditions, the free concentration of the dyes in the cytoplasm is low compared to the extracellular concen- trations of the dyes. Equilibration...
Ngày tải lên: 18/02/2014, 13:20
Tài liệu Báo cáo khoa học: A facile method for expression and purification of the Alzheimer’s disease-associated amyloid b-peptide pdf
... and D23N), and the availability of a rapid and simple expression and purification protocol will facilitate large-scale investigations of the molecular determinants of aggregation and fibrillation. ... solution. To minimize carbam- ylation of Ab, fresh solutions of ice-cold, deionized ACS grade urea were used, and the duration of exposure to urea was limited to less than...
Ngày tải lên: 18/02/2014, 13:20
Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx
... sig- nal for the determination of the fraction of folded or unfolded DNA strand, allowing for the calculation of the equilibrium distribution of conformational forms. Standard methods using UV absorbance ... a thorough understanding of not only their structures, but also of the energetics of their folding reactions and conformational transitions among different forms. The st...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: A study on genomic distribution and sequence features of human long inverted repeats reveals species-specific intronic inverted repeats pptx
... gene expression. The stem-loops and palindromes constructed by inverted repeats are involved in RNA interference, transcription initiation of genes, initiation of DNA replication and alternative ... Considering that the LIRs probably have essential functions and drive the speciation of primates, we studied the degree of conservation and species specification of the LIRs among...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... flasks and purified by a combination of ion- exchange chromatography, Lentil-Lectin Sepharose chro- matography and chromatofocusing, as described previously [21]. Results Expression and secretion of ... BigDye Terminator Cycle Sequencing Ready Reaction Kit v2.0 (Perkin-Elmer) and an ABI Prism 310 Genetic Analyzer (PE Corporation). Transfection of insect cells and generation o...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Residues affecting the chloride regulation and substrate selectivity of the angiotensin-converting enzymes (ACE and ACE2) identified by site-directed mutagenesis pot
... is absent in ACE2 as a result of substitution of the tACE residues Pro407 and Pro519 with Glu398 and Ser511, and the resulting projection of their side-chains into the location of this site. Consequently, ... Gerothanassis IP (2003) Structure and function discrimination of the N- and C-catalytic domains of human angiotensin-converting enzyme: implications for Cl – activa...
Ngày tải lên: 16/03/2014, 04:20
Báo cáo khoa học: Structural insight into the evolutionary and pharmacologic homology of glutamate carboxypeptidases II and III ppt
... design of GCPIII-specific therapeutics and ligands, as well as in the investigation of the diverse biological functions of GCPIII and GCPII. Experimental procedures rhGCPIII and rhGCPII expression and ... previously [29]. For crystallization experiments, EPE was dissolved in distilled water to a final concentration of 50 mm. Crystallization, data collection and processing Init...
Ngày tải lên: 23/03/2014, 05:22