Báo cáo khoa học: Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin docx

... speciﬁcity of Jacalin-related lectins Comparative analysis of carbohydrate-binding properties of two tandem repeat-type Jacalin-related lectins, Castanea crenata agglutinin and Cycas revoluta leaf lectin Sachiko ... analysis of these large mJRLs, i.e. C. crenata agglutinin (CCA) and C. revoluta leave lectin (CRLL)revealed that they form a tandem- repeat structurecomposed of two jacalin-type CRDs [21,22]. Therefore,mJRLs ... equippedwith an MS detector for analysis of mushroom lectins, and demonstrated its efﬁciency [28,29].In this present study we applied two tandem repeat-type mJRLs, CCA and CRLL, to this automated...

Báo cáo khoa học: Comparative analysis of the site-speciﬁc N-glycosylation of human lactoferrin produced in maize and tobacco plants pdf

... deglycosylation of the fractions H1,M1 and T1showthe disappearance of peaks between 3800 and 4600 Da forhLf, and between 3000 and 3700 Da for the two recombin-ant lactoferrins. In contrast, ... N-glycosylation of maize and tobacco lactoferrin (Eur. J. Biochem. 270) 3237MALDI mass spectra of the glycopeptide fractions revealthe heterogeneity of these fractions suggesting severalglycoforms and/ or ... site.Structural analysis of N-glycopeptidesGlycopeptide-containing fractions were analysed byMALDI-TOF before and after N-glycanase treatments.Fig. 1. Fractionation of tryptic digests of natural and recombinantlactoferrins...

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... UÆmg)1protein and recovery levels of 20–30%. In all cases, the analysis by SDS ⁄ PAGE and native PAGE of puriﬁed preparations showed onlyone protein band of 20–22 kDa (Figs 1 and 2; supple-mentary ... Rao and Dr J.Josse for critical review of the manuscript, and Profes-sor W. Lo¨ffelhardt (University of Vienna, Austria) forhis assistance in N-terminal protein sequencing and some MALDI-TOF ... mass of 19 216 Da and a pI of 4.69, in good agreement withMALDI-TOF MS (Fig. 1A) and chromatofocusingdata, respectively. The other three N-terminalsequences determined in this work, of Anabaena...

Báo cáo khoa học: Proteome analysis at the level of subcellular structures Mathias Dreger pot

... gene expression.For comparative studies, in order to elucidate themolecular basis of biological processes, the analysis of dynamic changes of the subcellular distribution of geneproducts is ... different kinds of informationexceeding the mere identiﬁcation of proteins. Thesecomprise of studies on different part of the nucleus of eukaryotic cells to demonstrate how proteome analysis can ... puriﬁcation, the issue of analysis of multiproteincomplexes will be discussed in an accompanying minireview[9a].Proteome analysis of subnuclear structures:the functional architecture of a complexorganelleThe...

Tài liệu Báo cáo khoa học: The antibacterial and antifungal properties of trappin-2 (pre-elaﬁn) do not depend on its protease inhibitory function pptx

... and the CFU counted. The per-centage of surviving CFU was calculated by the formulaN ⁄ Ncontrol· 100, where N and Ncontrolwere the numbers of CFU obtained after 3 h of incubation with and ... (2006)Secretion of serine peptidase by a clinical strain of Candida albicans: inﬂuence of growth conditions and cleavage of human serum proteins and extracellularmatrix components. FEMS Immunol Med ... exacerbations of COPD. Infection 35, 143–149.38 Higuchi R, Krummel B & Saiki RK (1988) A generalmethod of in vitro preparation and speciﬁc mutagenesis of DNA fragments: study of protein and DNA...

Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiCstructures.Despite the deletion of the B-loop, the two putativecatalytic residues Glu68 and Glu77 ... violet) and a small, presumablyamorphous, subfraction of a-chitin and b-chitin, but was not capable of degrading crystalline chitin completely. The crystal structures of chitinaseG and a related ... thatcatalyze the hydrolysis of chitin, a carbohydrate poly-mer of 1,4-b-linked GlcNAc. Chitin is found in thecuticle of insect shells, in shells of crustaceans, and inthe cell walls of many fungi, making...

Báo cáo khóa học: Localization, puriﬁcation and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot

... molecular mass of 103 kDa (composed from two 52 kDa monomers). The puriﬁed enzyme had optimumactivity at pH 3.0 and 40 °C and an isoelectric point of 9.8. The periplasmic localization of the enzyme ... decomposition of tetra-thionate was proposed by Steudel et al.[16],Hazeuet al.[17] and Meulenberg et al. [18] in experiments with intactcells of A. ferrooxidans and A. acidophilum.Propertiesofpuriﬁed ... precipitation of the enzymeat low ionic strength was not observed.HPLC analysis of reaction products demonstrated thepresence of thiosulfate and pentathionate. In further work,the stoichiometry of the...

Báo cáo khoa học: Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia coli transthyretin-related protein potx

... 2011Stability and ﬁbril formation properties of human and ﬁsh transthyretin, and of the Escherichia colitransthyretin-related proteinErik Lundberg1, Anders Olofsson2, Gunilla T. Westermark3 and ... molecular basis of thermalstability from the analysis of ion-pair networks in theglutamate dehydrogenase family. Eur J Biochem 255,336–346.68 Sonnhammer EL & Durbin R (1997) Analysis of protein ... wehave investigated the ﬁbril-forming properties of sbTTR and ecTRP in vitro, and compared the resultswith those of hTTR.Our results showed that hTTR, sbTTR and ecTRPcan form ﬁbrillar structures,...

Báo cáo khoa học: Ligand binding and antigenic properties of a human neonatal Fc receptor with mutation of two unpaired cysteine residues docx

... Søren Andersen and ProfessorPeter Roepstorff (University of Southern Denmark,Odense) for recording the presented MALDI-TOF ⁄TOF spectrum, and Tove Olafsen and Anna M. Wu(University of California ... Buus2 and Inger Sandlie11 Department of Molecular Biosciences and Centre for Immune Regulation, University of Oslo, Norway2 Institute of Medical Microbiology and Immunology, University of Copenhagen, ... half-life of Fig. 4. Analysis of b2m and hFcRn HCs byMALDI-TOF MS prior to reduction of disulﬁde bonds (aliquot 1). Spectra wererecorded after iodoacetamide treatment and tryptic digestion of gel-separated...

Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx

... the two redox states of thecofactor and that the distances from Ser23O to O1¢ and O2¢are slightly different (Table 1) because of the differentpositions and hybridizations of the C6 atom of ... hydroxylation of the cosubstrates [21,41].However, the sequence of binding of cofactor and substrateis still a matter of discussion and may be diﬀerent comparingwild-type and mutant forms of the ... surface plasmon resonance analysis, and the rate of phosphorylation of Ser16 by cAMP-dependent protein kinase. Comparison of the inhibitory properties of the natural cofactor with the available...

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