Báo cáo khoa học: The yeast stress response Role of the Yap family of b-ZIP transcription factors The PABMB Lecture delivered on 30 June 2004 at the 29th FEBS Congress in Warsaw potx

... REVIEW ARTICLE The yeast stress response Role of the Yap family of b-ZIP transcription factors The PABMB Lecture delivered on 30 June 2004 at the 29th FEBS Congress in Warsaw Claudina Rodrigues-Pousada, ... regulated by Yap4 , shows a further decrease in gene expression in the dou- ble yap4 yap6 mutant strain, suggesting cooperat...

Ngày tải lên: 30/03/2014, 16:20

... hydrophilic pravastatin) at a concentration of 30 lm mobilize free cytoplasmic calcium [Ca 2+ ] i to 30 50% of that induced by Bk at a concentration of 10 nm. In the case of simvastatin and lovastatin, this ... the in vivo assay of endothelial function [18–20,29,59,67–70]. In our in vivo method it is not the vasodilator response (as in the case of FMD i...

Ngày tải lên: 07/03/2014, 21:20

... translocation of the profission protein dynamin-related protein 1 (Drp1) was observed following HF-LPLI, demonstrating apoptosis-related activation of Drp1. Notably, overexpression of Drp1 increased ... 2A,B). These data demonstrate that HF-LPLI induces mitochondrial fragmentation by triggering oxi- dative stress. We also investigated the correlation between the laser fluence of...

Ngày tải lên: 29/03/2014, 00:20

... roles in the complex network that controls cell growth and proliferation, the elucidation of the molecular path- ways that transmit information on the growth state of a cell population to the Pol I transcription ... stimulates Pol I transcription in part through phosphorylation of the C-terminal activation domain of UBF [28], under- scoring the importance of...

Ngày tải lên: 29/03/2014, 21:20

... preserves information about the position of the predicate. The template also includes information about the voice of the predi- cate. For example, this template will be instantiated as follows for the ... variation of this feature uses the actual predicate lemma in addition to “voice:active”. Additionally, we define variations of these feature templates that concatenate...

Ngày tải lên: 08/03/2014, 04:22

... reported to upregulate the calci- neurin-dependent degradation of Yap1 p [29], the main transcription factor inducing gene expression in response to oxidative stress [19,20 ,30] . If the high cyto- solic ... When used, the calcium che- lator BAPTA was added to the cell suspension to the final concentration of 5 mm 1 min before application of the stress. To ensure...

Ngày tải lên: 15/03/2014, 23:20

... Differences in the molecular mechanisms involved in the transcriptional activation of the CHOP and asparagine synthetase genes in response to amino acid deprivation or activation of the unfolded protein ... Expression vector pET -30 ⁄ At SEL-1L allows the fusion of the histidine tag LEHHHHHH to the C-terminus of a recombinant protein. Expression and purificatio...

Ngày tải lên: 07/03/2014, 21:20

... lipid-soluble antioxidant that prevents the oxidative damage of lipids due to peroxidation [1–4]. Studies using ubiquinone-deficient yeast mutants also suggested that one of the in vivo functions of ubiquinone is ... generated by the formation of protein disulfide in E. coli [12]. Thus, ubiqui- none appears to play multiple roles. The ubiquinone biosynthetic pathway is com...

Ngày tải lên: 23/03/2014, 21:20

... centrifuged at 12 000 g for 10 min at 4 °C. The pellet, which contained the mitochondria, was resuspended in 30 lL of Mito-Cyto Buffer. The protein concentration was determined using the bicinchoninic ... – 80 °C until further use. The concentration of the protein was estimated using the bicinchoninic acid assay. Cytochrome c release in CCCP-treated mitochondria Mi...

Ngày tải lên: 16/02/2014, 09:20

... with CUL3 and other BTB proteins [2,16], it remains unclear what kind of interactions are mediated by the MATH domain in plants. In this article, we show that the MATH domain of BPM proteins is used ... are often transcriptional regulators containing a C 2 H 2 domain for DNA binding, but can also be found in combination with various other protein–protein inter- action motifs, su...

Ngày tải lên: 18/02/2014, 06:20