... 13. 9% 14 .3% 26.9% D93A 44 .3% 14. 7% 12.6% 25.8% D159A 57.5% 10.6% 11 .4% 22 .3% D 93 ⁄ 159 A 50 .3% 12 .3% 12.6% 24. 5% TBS + 2.2 m M Ca 2+ WT 38 .7% 15. 6% 14. 4% 27.6% D93A 40 .2% 15. 9% 13. 5% 27.2% D159A ... EP 24 .15 secondary structure (Fig. 8) and A WT 93 159 93 /159 4 - Ca 2+ + Ca 2+ 3 2 1 0 WT D93A D159A D 93 /159 A EP 24 .15 UAF/min 93 159 93 /159...
Ngày tải lên: 30/03/2014, 16:20
... (1995) Phospholipase A 2 engineering. The roles of disulfide bonds in structure, conformational stability, and cata- lytic function. Biochemistry 34 , 1 53 07 1 53 14. 31 Huang B, Yu BZ, Rogers J, Byeon IJ, Sekar K, Chen X, ... the C-termi- nus segment changes substrate specificity and uncouples calcium and substrate binding at the zwitterionic inter- face. Biochemistry 35 ,...
Ngày tải lên: 06/03/2014, 11:20
Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties pdf
... m.i. b m.i. b m.i. b m.i. b 2 0.7 1.89 206 49 4 3 10.76 10.7 835 5 34 4 6.68 25.17 906 34 88 5 3. 82 7.79 919 1050 6 2. 64 6.72 2505 17 54 Normal values < 0 .45 < 0.25 < 80 < 200 a Maximal ... performed at 94 °C for 5 min followed by 35 cycles of denaturation at 94 °C for 30 s, annealing at 55.8, 59 .3 or 62.9 ° C for 30 s (62.9 °C for exons 1 and 11, 59 .3 °C f...
Ngày tải lên: 23/03/2014, 04:21
Báo cáo khoa học: Characterization of a hemocyte intracellular fatty acid-binding protein from crayfish (Pacifastacus leniusculus) and shrimp (Penaeus monodon) pdf
... 1 047 , 233 – 238 . 3 Maatman RG, Degano M, Van Moerkerk HT, Van Marrewijk WJ, Van der Horst DJ, Sacchettini JC & Veerkamp JH (19 94) Primary structure and binding characteristics of locust and ... Urol 1 73, 247 –251. 8 Noy N (2000) Retinoid-binding proteins: mediators of retinoid action. Biochem J 38 4, 48 1 49 5. 9 Chen Q & Ross CA (20 04) Retinoic acid regulates cell...
Ngày tải lên: 23/03/2014, 10:21
Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx
... (kcalÆmol )1 ). Parameters bB2-Crystallin (calcium- binding) bA3-Crystallin (terbium binding) K 1 (2 .15 ± 1 .3) · 10 )4 (1.08 ± 0.08) · 10 )4 K 2 (1.65 ± 0.98) · 10 )4 (1 .46 ± 0.09) · 10 )4 K 3 (8 .33 ± 7. 63) · 10 )5 (4. 03 ± 0 .3) · ... ± 0 .3) · 10 )5 K 4 (5.71 ± 4. 89) · 10 )4 (2.72 ± 0. 13) · 10 )3 DH 1 2.75 ± 0.65 2.76 ± 0.07 DH 2 4. 0 ± 1.0 2 .15 ± 0. 23 DH 3...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx
... Spain Fax: + 34 9 83 4 23 588 Tel: + 34 9 83 4 23 062 E-mail: jbalsinde@ibgm.uva.es (Received 26 December 2007, revised 15 February 2008, accepted 21 February 2008) doi:10.1111/j.1 742 -46 58.2008.0 635 0.x We ... 1. J Immunol 167, 34 14 34 21. 43 Mishra RS, Carnevale KA & Cathcart MK (2008) iPLA 2 b: front and center in human monocyte chemo- taxis to MCP-1. J Exp Med 205,...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Calcium-independent cytoskeleton disassembly induced by BAPTA pdf
... 4- (trifluoromethoxy)phenyl-hydrazone. (Received 1 9 April 20 04, revised 15 June 2 0 04, accepted 18 June 20 04) Eur. J. Biochem. 271, 32 55 32 64 (20 04) Ó FEBS 20 04 doi:10.1111/j.1 43 2 -1 033 .20 04. 042 59.x D-BAPTA ... of Rho and indu ce s focal adhesions. FEBS Lett. 40 4, 118– 1 24. 42 . Tsien, R.Y. (1981) A non-disruptive technique for loading calcium buffers and i nd...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Calcium signalling by nicotinic acid adenine dinucleotide phosphate (NAADP) doc
... Road, Oxford OX1 3QT, UK Fax: +44 1865 2718 53 Tel: +44 1865 271 633 E-mail: antony.galione@pharm.ox.ac.uk (Received 28 April 2005, accepted 30 June 2005) doi:10.1111/j.1 742 -46 58.2005. 048 60.x Nicotinic ... stores sensitive to Calcium signalling by NAADP M. Yamasaki et al. 46 04 FEBS Journal 272 (2005) 45 98 46 06 ª 2005 FEBS NAADP and cyclic ADP-ribose. J Cell Sci 1 13, 44 13...
Ngày tải lên: 20/02/2014, 01:20
Báo cáo khoa học: Lysophosphatidylcholine modulates fibril formation of amyloid beta peptide doc
... h 15 5 C 10 D 20 25 A β 1 -42 30 A β 1 -42 10 15 20 5 Tyrosine fluorescence Tyrosine fluorescence Tyrosine fluorescence Tyrosine fluorescence 2 h 10 280 30 0 32 0 34 0 0 h 0 248 24 h 0 248 24 0 E A β 1 -40 30 30 A β 1 -40 F GH 20 20 10 10 0 24 ... h A β 1 -42 40 0 500 600 0 40 60 20 0 0 248 24 h 0 248 24 h Wavelength (nm) 40 0 500 600 Wavelength (nm) Wav...
Ngày tải lên: 06/03/2014, 01:20
Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf
... have included the Drosophila TRP channel [ 239 ], TRPC1 and TRPC3 [ 240 ], TRPC6 [ 241 ], TRPC7 [ 242 ,2 43 ], and TRPM7 [159 ]. Mitochondrial permeabilization and a possible link to TRP channel activation Among ... and alpha-keto acid dehydrogenase complexes. Arch Biochem Biophys 249 , 2 63 272. Ca 2+ , mitochondria, ROS in neuronal disease C. Chinopoulos and V. Adam-Vizi 4...
Ngày tải lên: 07/03/2014, 12:20