Báo cáo khoa học: The two IQ-motifs and Ca2+/calmodulin regulate the rat myosin 1d ATPase activity pptx

... monitored for increasing concentrations of the ligand in the presence of the Par j proteins. Fluorescence data, measured in the titration of the two recombinant proteins and the natural mixture of 0.15 ... mediating the transport of lipids from their site of biosynthesis to other membranes. The presence of a signal peptide in their sequence, on the other hand, suggests a...

Ngày tải lên: 30/03/2014, 02:20

... The two IQ-motifs and Ca 2+ /calmodulin regulate the rat myosin 1d ATPase activity Danny Ko ¨ hler*, Sandra Struchholz* and Martin Ba ¨ hler Institute for General Zoology and Genetics, ... binding domain of rat myosin 1d consists of two IQ-motifs, both of which bind the light chain calmodulin (CaM). To analyze the Myo1d ATPase activity as a function...

Ngày tải lên: 30/03/2014, 16:20

... is the relative enzyme activity (rate in the presence of inhibitor ⁄ rate in its absence), [E] 0 and [I] 0 are the total enzyme and inhibitor concentrations, respectively, and K ¼ K i if the ... and referred to as trappin throughout this article; that inhibit NE and Pr3 [6], but not cathepsin G [3]). The two former proteins are mainly synthesized in the liver and r...

Ngày tải lên: 19/02/2014, 07:20

... [32]. The Lsm1 and Lsm8 polypeptides are most similar in the regions of the Sm1 and Sm2 motifs. These motifs form the Sm-fold, the hallmark of the Sm-like pro- teins, consisting of a five-stranded ... investigate the requirement for different domains of the Lsm1 and Lsm8 proteins in their function and localization, we created a series of mutant and hybrid proteins. We...

Ngày tải lên: 07/03/2014, 02:20

... of the loaded carrier – as the rate-limiting step – with delivery o f substrate and c otransported ion to the internal side. The lack of transport of the two neuroactive compounds D -Ser and ... internal membrane side is the rate-limiting s tep and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outsid...

Ngày tải lên: 07/03/2014, 16:20

... transport and little or no sensing activity, others that had transport and were constitutive, and still others that had sensing activity and no transport. Acknowledgements Work in the laboratory ... enterica and P. multocida); and D223 (that appears in all UhpC-like proteins including HPTcp, but not in the UhpT proteins). The G213V exchange altered both the transport...

Ngày tải lên: 08/03/2014, 08:20

... zinc-insertion activity and a slight increase in iron-removal activity. These results suggest that ferrochela- tase localizes to both the mitochondrial outer and inner membranes and that the change in the ... cells and mouse liver mitochondria. The conven- tional (zinc-insertion) activities of the enzyme in the inner and outer mem- branes were similar, whereas the i...

Ngày tải lên: 16/03/2014, 00:20

... 2). The V max and k cat values for the D1 S D2D3 mutant were significantly higher than the values for the D1D2 S D3 and D1D2D3 S mutants (values for the latter two were not different from each other). ... 1). There was a significant decrease of K S(PCr) in the D1D2 S D3 S mutant as well as of the K S(ADP) and K M(ADP) values for the triple mutant. The wild-type and a...

Ngày tải lên: 16/03/2014, 06:20

... the red fluorescent morphs of L. hemprichii and M. cavernosa, the orange morph of L. hemprichii, and extracts of the red alga Fauchea sp. and the cyanobacterium Lyngbya sp. for their cross-reactivity ... investigate whether the pigments responsible for the coloration are GFP-like proteins or other pigments such as phycoerythrin, we constructed cDNA libraries of two individu...

Ngày tải lên: 16/03/2014, 11:20

... of CO 2 (e.g. from either of the two c-carboxyglutamic acid residues or other acidic groups) rather than from synthetic by-products based on the RP-HPLC, ion exchange chro- matography and capillary ... validate the activity of the endo-O-glycosidase enzyme, which was un able to hydrolyze native tx5a. Together these data suggest that the glycan configuration of native tx5a is di...

Ngày tải lên: 23/03/2014, 13:20