Báo cáo khoa học: Protein dissection enhances the amyloidogenic properties of a-lactalbumin potx

... 691–703. Proteolysis of proteins enhances fibrillogenesis P. Polverino de Laureto et al. 2188 FEBS Journal 272 (2005) 2176–2188 ª 2005 FEBS Protein dissection enhances the amyloidogenic properties of a-lactalbumin Patrizia ... deletion of a chain segment of 12 amino acid resi- dues located at the level of the b-subdomain of the native protein (1– 40 ⁄ 53–123...

Ngày tải lên: 30/03/2014, 16:20

... comparable to that of Syn HO-1 in the presence of each of the reducing systems, although the ascorbate-supported activity of Syn HO-2 was stronger than that of Syn HO-1. The activity levels of both enzymes ... (2.67, 2.21, 1.79) of the alkaline form of heme-rHO-1 [28]. The g-values of the minor component of heme–Syn HO-2 seem to be similar to those of the...

Ngày tải lên: 30/03/2014, 15:20

... irrespective of the presence of the supernatant from the initial centrifugation, the yield of EJ products did not appreciably change (com- pare lanes 1 and 2). The replacement of the superna- tant ... Discussion Aberrant types of DNA coaggregate with nucleoproteins To know whether the assembly of nucleoproteins with DSBs is aggregative, we examined the precipitable...

Ngày tải lên: 19/02/2014, 06:20

... the condensation and fragmentation of their nuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... still largely unknown. The aim of the current study was to investigate the potential involvement of nucleolin ⁄ C23 in the antiapoptotic mechanism of Hsp70. We found...

Ngày tải lên: 16/02/2014, 09:20

... TM segment of a membrane protein can have its N-terminus on either side of the membrane, depending on the amino acid sequence of the protein, which often determines the orientation of subsequent TMs. ... detergent. Membrane protein integration During the synthesis of a membrane protein, hydro- phobic TM segments move from the aqueous interior of the channel th...

Ngày tải lên: 07/03/2014, 06:20

... in the slower amide exchange rates. Taking the results of the two sets of experiments together, and in view of the strong stabilizing effect the solute confers upon this protein and the lack of structural ... which agrees with the expected flexibility of the N-terminal region of the protein. The difference of S 2 values in the presence and absence of so...

Ngày tải lên: 07/03/2014, 21:20

... separately from the other TRs, because they signif- icantly outnumber the other types of repeats, and having them in the same group would obscure the effect related to the other repeats. The amino ... homorepeats and the other TRs show the same trend. With the increase in the perfection of the repeated segment, the amount of order-promoting residues is gradually...

Ngày tải lên: 15/03/2014, 11:20

... transfer, the efficiency of transfer is deter- mined both by the orientation of the energy transfer partners and by the distance between them [28]. The fact that the efficiency of transfer varies with the 6th-power ... further questions about the use of coimmunoprecipitation in the absence of further supporting evidence of protein protein inter- Fig. 1. Different...

Ngày tải lên: 16/03/2014, 22:20

... regulate the release of cytochrome c, Smac ⁄ Diablo and other proteins from mitochondria, or inhibit apoptosis to inactivate the apoptotic functions of the pro-apoptotic proteins to form a protein ... effect via the mitochondria by regulating the activities of pro- apoptotic and anti-apoptotic proteins and transloca- tion of these proteins to the mitochondria [8,9,24,32]...

Ngày tải lên: 23/03/2014, 07:20

... ubc9 and the UFD of uba2 interact at the a1 region. (b) The region below the a1 helix consists mainly of the N-terminal exten- sion of a1 and loops between the b2 and b3 strands and the b6 strand ... under- stand the mechanistic roles of these ligases. Future studies should also address the question of how SUMO proteins are translocated between their various sites...

Ngày tải lên: 30/03/2014, 04:20