Báo cáo khoa học: Protein dissection enhances the amyloidogenic properties of a-lactalbumin potx

... 691–703.Proteolysis of proteins enhances ﬁbrillogenesis P. Polverino de Laureto et al.2188 FEBS Journal 272 (2005) 2176–2188 ª 2005 FEBS Protein dissection enhances the amyloidogenic properties of a-lactalbumin Patrizia ... deletion of a chain segment of 12 amino acid resi-dues located at the level of the b-subdomain of the native protein (1–40 ⁄ 53–123, named desb-LA). We have also compared the early stages of the ... at the level of the b-subdomain of the 123-residue chain of the protein (Fig. 1). We have alsocharacterized the early stages of protein aggregation inorder to get insights into the molecular...

Báo cáo khoa học: Protein expressed by the ho2 gene of the cyanobacterium Synechocystis sp. PCC 6803 is a true heme oxygenase pot

... comparableto that of Syn HO-1 in the presence of each of the reducing systems, although the ascorbate-supportedactivity of Syn HO-2 was stronger than that of SynHO-1. The activity levels of both enzymes ... (2.67,2.21, 1.79) of the alkaline form of heme-rHO-1 [28]. The g-values of the minor component of heme–SynHO-2 seem to be similar to those of the major low-spin component (2.78, 2.14, 1.74) of heme–Syn ... isnot seen in the spectrum of the high-spin heme SynTable 1. Activities of the puriﬁed Syn HO-2. The HO activity wasdetermined from the initial rate of bilirubin formation with the NADPH ⁄ FNR...

Tài liệu Báo cáo khoa học: Aggregative organization enhances the DNA end-joining process that is mediated by DNA-dependent protein kinase pdf

... irrespective of the presence of the supernatant from the initial centrifugation, the yield of EJ products did not appreciably change (com-pare lanes 1 and 2). The replacement of the superna-tant ... DiscussionAberrant types of DNA coaggregate withnucleoproteinsTo know whether the assembly of nucleoproteins withDSBs is aggregative, we examined the precipitableproperty of the complex. The assay system ... assembly of nucleoproteins in human cellular fractions and thatthey mediate the separation of large protein DNA aggregates from aque-ous solution. The interaction among the aggregative nucleoproteins...

Tài liệu Báo cáo khoa học: Nucleolin/C23 mediates the antiapoptotic effect of heat shock protein 70 during oxidative stress pptx

... the condensation and fragmentation of theirnuclei. The percentage of apoptotic cells was calculated as the number of apoptotic cells divided by the total number of cells counted. A minimum of ... stilllargely unknown. The aim of the current study was to investigate the potential involvement of nucleolin ⁄ C23 in the antiapoptotic mechanism of Hsp70. We found that primary cultures of neonatal rat ... marked downregulation of Bcl-2 protein, and nucleolin overexpression could inhibit the down-regulation of Bcl-2 protein (Fig. 5A). Furthermore, wecompared the stability of Bcl-2 mRNA in cells...

Báo cáo khoa học: Protein transport across the endoplasmic reticulum membrane pdf

... TM segment of a membrane protein canhave its N-terminus on either side of the membrane,depending on the amino acid sequence of the protein, which often determines the orientation of subsequentTMs. ... detergent.Membrane protein integrationDuring the synthesis of a membrane protein, hydro-phobic TM segments move from the aqueous interior of the channel through the lateral gate into the lipidphase. The ... [16]. The aqueous interior of the channel and itsshape help to minimize the energy required for the translocation of a polypeptide through the membrane. The plug can only return to the center of...

Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx

... in the slower amideexchange rates. Taking the results of the two sets of experiments together, and in view of the strong stabilizingeffect the solute confers upon this protein and the lack of structural ... whichagrees with the expected ﬂexibility of the N-terminalregion of the protein. The difference of S2values in the presence and absence of solute are shown in Fig. 4. Overall, the average S2values ... solvent structure and/or subtle changes in the dynamic properties of the protein rather than by changing the structure of the protein itself. In fact, protein functiondepends vitally on its structure...

Báo cáo khoa học: Protein tandem repeats – the more perfect, the less structured pptx

... separately from the other TRs, because they signif-icantly outnumber the other types of repeats, andhaving them in the same group would obscure the effect related to the other repeats. The amino ... homorepeatsand the other TRs show the same trend. With the increase in the perfection of the repeated segment, the amount of order-promoting residues is graduallyreduced, whereas the relative contents of ... in eukaryotic proteins wouldobscure the results. Prior to the analysis, the redun-dancy of the dataset related to the existence of protein sequences from different strains of the same species(especially...

Báo cáo khoa học: Methods to monitor the quaternary structure of G protein-coupled receptors doc

... transfer, the efﬁciency of transfer is deter-mined both by the orientation of the energy transferpartners and by the distance between them [28]. The fact that the efﬁciency of transfer varies with the 6th-power ... further questionsabout the use of coimmunoprecipitation in the absence of further supporting evidence of protein protein inter-Fig. 1. Differentially epitope-tagged forms of the a1b-adrenoceptorhave ... over the distance resulting in 50% of the maximalefﬁcacy (R0). As indicated by the Fo¨rster equation, the efﬁciency of transfer varies inversely with the 6th-power of the distance. The extreme...

Báo cáo khoa học: Protein kinase Ce: the mitochondria-mediated signaling pathway pot

... regulate the release of cytochrome c,Smac ⁄ Diablo and other proteins from mitochondria, orinhibit apoptosis to inactivate the apoptotic functions of the pro-apoptotic proteins to form a protein ... effect via the mitochondria by regulating the activities of pro-apoptotic and anti-apoptotic proteins and transloca-tion of these proteins to the mitochondria [8,9,24,32].Involvement of PKCe ... Phosphorylation byPKCe enhances the activity of COIV, whereas the physical interaction of PKCe with components of the cardiac mitochondrial pore inhibits MPTP. Again,these lines of evidence provide...

Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf

... ubc9 and the UFD of uba2 interact at the a1 region. (b) The region below the a1 helix consists mainly of the N-terminal exten-sion of a1 and loops between the b2 and b3 strandsand the b6 strand ... under-stand the mechanistic roles of these ligases.Future studies should also address the question of how SUMO proteins are translocated between theirvarious sites of interaction with the sumoylationenzymes. ... E1 is composed of APPBP1 (APPbinding protein 1) and uba3. The domain structures of Aos1 and APPBP1 resemble the N-termini, and those of uba2 and uba3 resemble the C-termini, of the ubiqu-itin...

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