Báo cáo khoa học: Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins pdf

... Role of phosphorylation in p53 acetylation and PAb421 epitope recognition in baculoviral and mammalian expressed proteins Lorna J. Warnock, Sally A. Raines, Trevor R. Mee and Jo Milner YCR p53 ... recombinant p53 proteins were expressed in Sf9 insect cells by using the baculoviral system and were purified as described previously [15]. Both human...

Ngày tải lên: 30/03/2014, 16:20

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... mediator of lethal sepsis, triggering the production of proinflammatory cytokines and liver injury [13]. Therefore, the present study aimed to elucidate the role of KCs...

Ngày tải lên: 14/02/2014, 18:20

... have indi- cated that the CagA PY -induced cell elongation pheno- type also involves tyrosine dephosphorylation of cortactin, vinculin and ezrin, which are three well- known actin-binding proteins ... the amount of intracellu- lar CagA PY . Cortactin, ezrin and vinculin are all Src substrates, and Src inactivation causes dephosphoryla- tion of these proteins and is crucial f...

Ngày tải lên: 14/02/2014, 19:20

... between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in a mouse keratinocyte cell line upregulation of CERK expression by activa- tion of PPARb results in a decrease ... survival resulting from activation of PPARb. Activation of PPARb by LD induces CERK mRNA expression and increases CerK activity in SP1 cells To investigate the ro...

Ngày tải lên: 18/02/2014, 18:20

... prepared from CT and CT-B-injected rats (Fig. 3A). An increase in ARF content was observed in endosomal fractions isolated 5 min postinjection of CT-B, and this increase was maintained for up to 60 min (Fig. ... cathepsin D inhibitor pepstatin A partially inhibited, both in vivo and in vitro, the cAMP response to cholera toxin. This cathepsin D-dependent action of cholera to...

Ngày tải lên: 19/02/2014, 02:20

... vector by using Lipofectamine (Invitrogen). Cells were selected in neomycin, and clones were isolated and screened for Bcl-X L expression. Creation of TAM67-overexpressing cell line The dominant-negative ... transcription, were increased in response to EGF. Pretreatment of the cells with PD98059, an inhibitor of MAP kinase kinase, inhibited the EGF-induced c-Fos and c-Jun expr...

Ngày tải lên: 19/02/2014, 06:20

... chain of K22 participates in fosfomycin binding, thus providing a rationale for the loss of fosfomycin binding to the K22V and K22E mutant proteins. However, two other positively charged amino ... charged amino acid side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a single...

Ngày tải lên: 19/02/2014, 13:20

... on conformation of inhibitor main chain. Thus, different inhibitors differ not only in t he binding affinity, bu t a lso in the degree o f freedom of the inhibitor inside the binding tunnel. Some s pecial inhibitors ... that t he strain imposed on inhibitor during docking into the binding site of pr otease does not allow correct orientation of the hydroxyl group to catalytic aspart...

Ngày tải lên: 19/02/2014, 16:20

... five amino acids of the extracellular domain and devoid of any tag was not functional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag was found ... periods of times (0–60 min) at 37 °C. The concentration of the biotinylated Ig remaining on the cell surface was then quantified by measuring the binding of [ 125 I]Streptavidin. In...

Ngày tải lên: 21/02/2014, 00:20

... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the ... domain structure of the V. spino- sum tyrosinase in more detail, we determined protein stability...

Ngày tải lên: 06/03/2014, 11:20