Báo cáo khoa học: Preparation and characterization of geodin A bc-crystallin-type protein from a sponge pot

... 1035 Preparation and characterization of geodin A bc-crystallin-type protein from a sponge Concetta Giancola1, Elio Pizzo2, Antimo Di Maro3, Maria Vittoria Cubellis2 and Giuseppe D’Alessio21 ... 575–599.36 Barone G, Del Vecchio P, Fessas D, Giancola C &Graziano G (1993) THESEUS: a new software packagefor the handling and analysis of thermal denaturationdata of biological macromolecules. ... that of protein S rather than that of a mammalian crystallin such as cS-crystallin, a qualitative, indirect indication of a closer structuralrelationship between geodin and protein S. Geodin also...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ ... Remarks15¢-GG (A ⁄ T)CA (A ⁄ C)GG (A ⁄ T)AC (A ⁄ C)TT(C ⁄ T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate ... component of the nucleolus and sti-mulated by phosphorylation with CK2 and cdc2 protein kinases. Plant J 25, 9–17.32 Yadav N, Chandok MR, Prasad J, Bhattacharya S,Sopory SK & Bhattacharya A (1997)...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... withinthe last 30 years. The greatest number of RIPs havebeen found in the Caryophyllaceae, Sambucaceae,Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially ... Roberts3 and Ana Paula U. Arau´jo1,21 Programa de Po´s-graduac a o em Gene´tica e Evoluc a o, Universidade Federal de Sa˜o Carlos, Brazil2 Instituto de Fı´sica de Sa˜o Carlos, Universidade ... R, Maiti TK & Podder SK (1991) Puriﬁcation and characterisation of three toxins and two agglutinins from Abrus precatorius seed by using lactamyl-Sepharoseafﬁnity chromatography. Anal Biochem...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... rounds and tested for tyrosinase activity with a plate assay. In the assay plates, Trichoderma minimal med-ium [19] with 2% lactose as a carbon source, 1% potassiumphthalate as a buffering agent ... fungaltyrosinases, from both a structural and a functionalpoint of view, are from Agaricus bisporus [10] and N. crassa [1]. Also, a few bacterial tyrosinases havebeen reported, of which Streptomyces tyrosinases ... tyro-sinases from N. crassa [26–28] and Agaricus [28] and Pycnoporus species [25] have an additional C-terminaldomain that is proteolytically released from the cata-lytic domain. It has been...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... follows: HSF 1a forward,5¢-GAAGCAGCTTGTCCAGTACACCAA-3¢; HSF 1a reverse, 5¢-TTCCAAGAGCTGAACAAACCATTG-3¢;HSF1b forward, 5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢; HSF1b reverse, 5¢-GGCTGAATAAACCATGCCAGTAGC-3¢; ... translations was analysed as a negative control (lanes 1 and 4), and vectors encoding epitope-taggedb-galactosidase (HA-bgal or Protein C-bgal) were translated in vitro aspositive controls (lanes 2 and ... toextract total RNA for RT-PCR, were obtained from theNikko Branch of the National Research Institute of Aquaculture (Tochigi, Japan) and reared on a commercialdiet at 15 °C.Cloning of HSFcDNAA...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... inves-tigation because of their physiological importance and their pharmaceutical relevance as drug carriers [1–6].Both transporters catalyse the uptake of most dipep-tides and tripeptides and a variety ... was inhibited not only byunlabeled Bip-Pro itself, but also by well known sub-strates of H+⁄ peptide cotransporters, such as Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic ... amount of Bip-Pro in the extracellular uptake mediumwas quantiﬁed according to the laboratory standard HPLC(La-ChromÒ; Merck-Hitachi, Darmstadt, Germany) with a diode array detector and a...

Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx

... library of protein variantsbased on the Q14 3A hMnSOD template. One hundred and ﬁfty thousand QC774 transformants were screenedon 40 M63 minimal media agar plates containing 1 lmparaquat for each ... of Illinois at Urbana-Champaign, Urbana, IL, USA2 Department of Pharmacology and Biochemistry, University of Florida, Gainesville, FL, USA3 Department of Neuroscience, University of Florida, ... Engineering and characterization of human manganesesuperoxide dismutase mutants with high activity and lowproduct inhibitionKaruppiah Chockalingam1,*, James Luba2,*, Harry S. Nick3, David N....

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... and XhoIML3p A- chain5¢-GATATACATATGTACCGTCGTATTAGCCTTCGTGTCACGGAT-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI and EcoRIML3.1p A- chain5¢-GATATACATATGTACGAGCGTCTTCGTCTTCGTGTTACGCATC-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI ... and XhoIML2p A- chain5¢-GATATACATATGTACGAGCGTCTTCGTCTTCGTGTTACGCATC-3¢5¢-CACACCTCGAGTTATTAAGAAGAAGACGGACGGTCCCGGCATAC-3¢NdeI and XhoIML3p A- chain5¢-GATATACATATGTACCGTCGTATTAGCCTTCGTGTCACGGAT-3¢5¢-CACACGAATTCTTATTAAGAAGAAGAAGAACGGTCCCTGCATAC-3¢NdeI ... cloning,5¢-AAAAATGCATGAAGTTGATTGCTTGCATTAACTCAT-3¢ (3¢UTR), 5¢-AAAAATGCATAGGGATGAAGTTGATTGCTTGCC-3¢ (3¢UTR) containing the NsiIrecognition site for cloning, and 5¢-CACAAGGTGGCTAAGGCTTCTTCCG-3¢...

Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx

... iscomposed of 7 parallel b strands associated to an anti-parallel strand (b2) and is surrounded by 5 helices (a1 , a2 , a3 , a7 anda8). T he second domain c onsists of helices a4 , a5 and a6 a ll clustered ... and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL familySte´phane Canaan1, Damien Maurin1, Henri Chahinian2,Be´ne´dicte ... connecting strandb3 to helix a1 and two small h elices (a4 anda6).The catalytic site consists of a functional catalytic triadfound in all serine enzymes of the a/ b hydrolase fold familyin which...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... act as an NADH oxidase in vivo, instead act-ing as a CoADR. This is only the second demonstra-ted CoA reductase activity, and the ﬁrst appearance of this activity in both the Archaea and in a ... concentration(as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a signiﬁcant level of identityto putative NADH oxidases from hyperthermophiles and bacterial NADH oxidases ... treated with dithiothreitol (5 mm) to reducethe disulﬁdes, and assayed as above. Thiols were quanti-ﬁed by comparison of peak areas to standard curves of those standards. Coenzyme A was measured...

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