... remains uncer-tain.To summarize, analysis of individual amino acidsubstitutions, coupled with molecular modeling of protein structure, indicate that b-strand 7 of the a-crystallin domain is ... localized in sucrose gradients by measuring the A280 of fractions.p26 induced thermotolerance in E. coliTransformed E. coli, incubated overnight with shaking at37 °C in 2 mL of LB medium, containing ... the regulation of the molecularchaperone Hsp26 by temperature-induced dissociation: the N-terminal domain is important for oligomer assem-bly and the binding of unfolding proteins. J Biol Chem279,...