Báo cáo khoa học: Structural determinants of protein stabilization by solutes The importance of the hairpin loop in rubredoxins pdf

... temperature [14]. Therefore, the tendency to increase the coefficients in the presence of the stabil- izing solutes reflects the strengthening of the hydrogen bond network. These findings are in line with ... Journal 272 (2005) 999–1011 ª 2005 FEBS 1011 Structural determinants of protein stabilization by solutes The importance of the hairpin loop...

Ngày tải lên: 30/03/2014, 15:20

... critical in determining the specificity of the bacterial sugar-binding proteins. Evaluation of the binding affinities by fluorescence measurements The K d values of TvuCMBP for binding of sugars ... parts of the sugar-binding sites of TvuCMBP and related proteins As the sugar-binding site is formed by N- and C-domains, residues involved in sugar binding are groupe...

Ngày tải lên: 19/02/2014, 00:20

... on actin aggregation in the course of thermal denaturation of F-actin. Previous studies have shown that the DLS method allows determination of the size of particles formed in the process of protein ... Hsp27-3D [2]. In the absence of Hsp27-3D, thermal denaturation of F-actin was accompanied by a strong increase in light scattering, whereas in the presen...

Ngày tải lên: 16/03/2014, 06:20

... maintained. Detection of the products of substrate oxygenation by the adsorbed MMOH was achieved by holding the potential at )0.5 V for 30 min in the presence of substrate and then removing the ... altering the redox potential of the binuclear iron site [6], optimizing the interaction between MMOR and MMOH [7], controlling the access of large substrates to t...

Ngày tải lên: 17/03/2014, 09:20

... chain takes place at the second glycosidic linkage from the MeGlcA side group towards the reducing end of the xylan chain. The elucidation of the three-dimensional structure of the E. chrysanthemi ... nonreducing end of the leaving group. A stereo view of the mode of binding of MeGlcA 2 Xyl 3 is shown in Fig. 5A. The interactions of the enzyme with MeGl...

Ngày tải lên: 22/03/2014, 16:20

... Alignment of the amino acids involved in the interactions with the first transcribed nucleoside (Guo) in the hDcpS–m 7 GpppG complex. These amino acids are not conserved in the other DcpS proteins illustrated. ... Mutation of the indicated amino acids in hDcpS to Ala only decreases slightly the enzymatic activity of the human scavenger [20]. (B) Alignment of th...

Ngày tải lên: 29/03/2014, 09:20

... for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72, 248–254. 28 Page R & Stevens RC (2004) Crystallization data min- ing in ... N7 (Fig. 1C). The 2¢OH of the ribose is hydro- gen bonded to the main chain and the side chain of Asp142 (Fig. 1C). Both hydroxyl groups of the ribose are involve...

Ngày tải lên: 15/02/2014, 01:20

... from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the free energy of binding as a function of the changes in buried surface ... promote other interactions in the presence of an external agent (lipids, peptides, other regions of the Gag protein, or even other proteins). In the...

Ngày tải lên: 07/03/2014, 06:20

... remains uncer- tain. To summarize, analysis of individual amino acid substitutions, coupled with molecular modeling of protein structure, indicate that b-strand 7 of the a-crystallin domain is ... localized in sucrose gradients by measuring the A 280 of fractions. p26 induced thermotolerance in E. coli Transformed E. coli, incubated overnight with shaking at 37 °C in 2 m...

Ngày tải lên: 07/03/2014, 12:20

... model of the structural organization of SV-IV protein. The amino-acid sequence was analyzed using the BLAST program to find similar proteins in the ÔnrÕ database (nonredundant database consisting of ... analysis. The corresponding start-end position in the protein sequence is indicated in the figure. Some differences in the hydrolytic pathways were found. The...

Ngày tải lên: 07/03/2014, 14:20