Báo cáo khoa học: Determination of the metal ion dependence and substrate speciﬁcity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

... Determination of the metal ion dependence and substrate speciﬁcity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl Pan Wang and Stephen Y. K. SeahDepartment of ... the metal ion has a cata-lytic rather than just a substrate binding role. Possibleroles of the cation include the activation of water for the hydration reaction and ⁄ or the stabilization of ... ishexa-coordinated in the enzyme. The nature of the metal ion affected only the kcat and not the Kmvalues in the BphH hydration of HPDA, suggest-ing that cation has a catalytic rather than just a substrate...

Báo cáo khoa học:Chính sách đối ngoại của Đảng với khu vực Đông Nam Á những năm đầu thế kỷ 21 pot

... trong dien van khai mac Hgi nghj Bg trudng Ngoai giao cac nudc ASEAN ldn thfl 34 (AMM 34) diin ra tai Ha Ngi, Thfl tudng Phan Van Khai khang djnh: "Chinh sach ASEAN Id ... DANG LANH OAO CONG CUOC DOI MOI CHINH SACH DOI NGOAI CUA DANG V6l KHO VlfC DONG NAM A NHfiNG NAM DAU THE KY XXI ThS NGUYEN THIHOAN Phien hgp todn the Diin ddn khu vuc ASEAN ... nguyen tac cfla ASEAN, cac gid trj ASEAN; gifl dugc cdch tiep can nang dgng, tinh tao vd cdn bdng, tilp tuc quan tdm thich ddng din viec thuc hien muc tieu cfla ASEAN, nhat la vl tang cudng...

Tài liệu Báo cáo khoa học: Evidence for noncooperative metal binding to the a domain of human metallothionein ppt

... domain of human MT- 1a [29] showed that the single remaining metal ion in the demetallationreaction was the C-terminal metal ion, indicating thatoccupancy of this metal site resulted in the least ... con-stants for the a domain are greater than those for the b domain may be a factor in explaining the observed metal ion distribution. After coordination of the ﬁrsttwo metal ions to the a domain ... Co2+indicated a simulta-neous metallation of the a and b domains, with two metal ions populating the a domain, and one in the bdomain [25]. All three of these metal ions were shownto bind to independent...

Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc

... 893, indicating the Fuc is at the internal 3,4-disubstituted GlcNAc. The absence of a 0,2 A 4a doublet and the presence of a D4f- 4a ion at m/z 729 (see the fragmentation scheme in Fig. 2)are in ... 2B),conﬁrming that the 3-linked disaccharide branch is the Table 1. Linkage and monosaccharide composition assignment from methylation analysis of milk oligosaccharides. PMAA, partially methylatedalditol ... Fuc in each of the 3- and 6-branches.Comparison of the product -ion spectra of the singly and doubly charged molecular ion precursor (Fig. 3A, B)show the major additional ions in the doubly charged...

Báo cáo khoa học: Determination of the reopening temperature of a DNA hairpin structure in vitro pptx

... denaturation and renatur-ation manipulations, the fast annealing (fast renaturation) and slow annealing (slow renaturation) procedures(Materials and methods) were performed after the primer2 and ... for the bacterial cultivation and M9 minimal medium was usedto maintain F¢ factors in E. coli strains [18]. Allmanipulations were following standard methods [18],unless indicated.Plasmid pTac5 ... todetect the DNA hairpin reopening temperature. A singleDNA hairpin structure was formed on the DNA templateby thermal denaturation and renaturation, and this hairpinstructure w as p redicted...

Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc

... catalytic activity of the NR1-FAD/NADPH domainwas examined and compared with the CPR FAD/NADPHdomain. The CPR FAD/NADPH domain retains trans-hydrogenase activity, and is capable of reducing ... further steady-statekinetic assays of potassium ferricyanide reduction. The ferricyanide was maintained at saturating concentration(250 lM), and NADPH concentration was varied between0.5 and ... 2B). The fully oxidized FMN domain had absorbancemaxima at 376 and 454 nm, and the FAD/NADPHdomain absorbed maximally at 376 and 453 nm. The FMN domain was stable for several weeks upon storage...

Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx

... CTCTTTGATGACAGAAGTGCCT-3¢Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 3655¢-450 AAAAATGTTGCTGCCCTG-3¢Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 4885¢-1054 CCTTTGAGCACATTTCGGCAA-3¢P450 aromatase ... CT, USA).DNA quanti®cation The DNA content of the cell layer at the end of incubationwas quanti®ed by the method of West et al .[31].Aftersolubilization of the cell layer in 1MNaOH and s ... 5¢-1555GCTTCTCATCGCAGAGTATCCGG-3¢ 2895¢-1821CAAGGGTAAATTCATTGGGCTTGG-3¢b-actin 5¢-2350 ACAGACTACCTCATGAAGAT-3¢ 6655¢-3222 AGCCATGCCAAATGTCTCAT-3¢Fig. 1. Dose-related eect of bFGF on FSH-stimulated...

Báo cáo khoa học: Determination of thioxylo-oligosaccharide binding to family 11 xylanases using electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and X-ray crystallography pot

... a function of the free ligand concentration and the ﬁt to Eqn (3) (solid line), obtained in the case of TRX I and S-Xyl5-Me. On the basis of the K a obtained, one can calculate the binding free ... portion of the substrate; Fig. 1) speciﬁcity. However, inter-actions of the substrate with the aglycone subsites playan important role in transition-state stabilization along the catalytic pathway ... disregarded in the determination of binding constants because of theirlow abundance and the fact that no peaks representingdimers were actually observed at ligand concentrationshigher than 20...

Báo cáo khoa học: " Determination of Syntactic Functions in Estonian Constraint Grammar " docx

... the last module in detail. Grammatical features of words are presented in the forms of tags which are at- tached to words. The tags indicate the inflectional and derivational properties of ... sufficiently reliable (robust) and the re- sulting syntactic analysis that the Grammar gives suits various practical applications. 2 Syntactic Analysis of Estonian The Estonian language is a Finno-Ugric ... properties of the word and the word class membership, the tags attached during the last stage of the analysis indicate its syntactic functions. The underlying principle in determin- ing both the morphological...

Báo cáo khoa học: Synechocystis DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the Fenton reaction potx

... of FAD), the reaction resulted in complete degradation of the DNA (no band was detected in Fig. 2, lane V). A partial DNA degradation was observed in the absence of iron compound (Fig. 2, lane ... preparation also showed a similar absorption maximum, conﬁrming the associ-ation of FMN with DrgA protein. The absorptionmaximum at 459 nm disappeared upon addition of 0.3 mm NADH under anaerobic ... DrgA protein functioning as nitroreductase and ferric reductase is capable of catalyzing the FentonreactionKouji Takeda1, Mayumi Iizuka1, Toshihiro Watanabe2, Junichi Nakagawa1, Shinji...

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