Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot

... Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl Pan Wang and Stephen Y. K. Seah Department of ... the metal ion has a cata- lytic rather than just a substrate binding role. Possible roles of the cation include the activation of water fo...

Ngày tải lên: 30/03/2014, 15:20

... trong dien van khai mac Hgi nghj Bg trudng Ngoai giao cac nudc ASEAN ldn thfl 34 (AMM 34) diin ra tai Ha Ngi, Thfl tudng Phan Van Khai khang djnh: "Chinh sach ASEAN Id ... DANG LANH OAO CONG CUOC DOI MOI CHINH SACH DOI NGOAI CUA DANG V6l KHO VlfC DONG NAM A NHfiNG NAM DAU THE KY XXI ThS NGUYEN THIHOAN Phien hgp todn the Diin ddn khu vuc ASE...

Ngày tải lên: 15/03/2014, 14:20

... domain of human MT- 1a [29] showed that the single remaining metal ion in the demetallation reaction was the C-terminal metal ion, indicating that occupancy of this metal site resulted in the least ... con- stants for the a domain are greater than those for the b domain may be a factor in explaining the observed metal ion distribution. After coordinat...

Ngày tải lên: 19/02/2014, 00:20

... 893, indicating the Fuc is at the internal 3,4-disubstituted GlcNAc. The absence of a 0,2 A 4a doublet and the presence of a D 4f- 4a ion at m/z 729 (see the fragmentation scheme in Fig. 2) are in ... 2B), confirming that the 3-linked disaccharide branch is the Table 1. Linkage and monosaccharide composition assignment from methylation analysis of milk oli...

Ngày tải lên: 19/02/2014, 12:20

... denaturation and renatur- ation manipulations, the fast annealing (fast renaturation) and slow annealing (slow renaturation) procedures (Materials and methods) were performed after the primer 2 and ... for the bacterial cultivation and M9 minimal medium was used to maintain F¢ factors in E. coli strains [18]. All manipulations were following standard methods [18], unless ind...

Ngày tải lên: 23/03/2014, 13:20

... catalytic activity of the NR1-FAD/NADPH domain was examined and compared with the CPR FAD/NADPH domain. The CPR FAD/NADPH domain retains trans- hydrogenase activity, and is capable of reducing ... further steady-state kinetic assays of potassium ferricyanide reduction. The ferricyanide was maintained at saturating concentration (250 l M ), and NADPH concentration was varie...

Ngày tải lên: 23/03/2014, 21:20

... CTCTTTGATGACAGAAGTGCCT-3¢ Syndecan-4 5¢-85 GAGTCGATTCGAGAGACTGA-3¢ 365 5¢-450 AAAAATGTTGCTGCCCTG-3¢ Glypican-1 5¢-566 GAATGACTCGGAGCGTACACTG-3¢ 488 5¢-1054 CCTTTGAGCACATTTCGGCAA-3¢ P450 aromatase ... CT, USA). DNA quanti®cation The DNA content of the cell layer at the end of incubation was quanti®ed by the method of West et al .[31].After solubilization of the cell layer in...

Ngày tải lên: 21/02/2014, 03:20

... a function of the free ligand concentration and the fit to Eqn (3) (solid line), obtained in the case of TRX I and S-Xyl5-Me. On the basis of the K a obtained, one can calculate the binding free ... portion of the substrate; Fig. 1) specificity. However, inter- actions of the substrate with the aglycone subsites play an important role in transition-st...

Ngày tải lên: 07/03/2014, 17:20

... the last module in detail. Grammatical features of words are presented in the forms of tags which are at- tached to words. The tags indicate the inflectional and derivational properties of ... sufficiently reliable (robust) and the re- sulting syntactic analysis that the Grammar gives suits various practical applications. 2 Syntactic Analysis of Estonian The...

Ngày tải lên: 24/03/2014, 03:21

... of FAD), the reaction resulted in complete degradation of the DNA (no band was detected in Fig. 2, lane V). A partial DNA degradation was observed in the absence of iron compound (Fig. 2, lane ... preparation also showed a similar absorption maximum, confirming the associ- ation of FMN with DrgA protein. The absorption maximum at 459 nm disappeared upon addition...

Ngày tải lên: 30/03/2014, 09:20