Báo cáo khoa học: Role of Tyr84 in controlling the reactivity of Cys34 of human albumin potx

Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx

... this binding. Some of theseFig. 10. Prediction of nucleolin-binding sites in human lactoferrin (hLf).Sequences of the N2 and C2 domains of hLf, bovine lactoferrin (bLf)and human transferrin (hTf) ... on the binding of HB-19. Similarly, the iron-binding hTf had no effect. On the other hand, bLf inhibited the binding of HB-19 to cells. In accordance with the involvement of both lobes of hLf in the ... Properties of the iron-binding site of the N-terminal lobe of human and bovine lactotransferrins. Importance of the glycanmoiety and of the non-covalent interactions between the N- andC-terminal...

Báo cáo khoa học: Role of Tyr84 in controlling the reactivity of Cys34 of human albumin potx

... Structural features of the Cys34 site in human serum albumin. (A) Domain structure of human serum albumin and location of Cys34. The coordinates used are those of pdb 1AO6 (albumin isolated from ... dramatic increase in reactivity for the Tyr84Phemutant. We also report1H NMR studies of disulﬁde interchange reac-tions with cysteine. The tethering of the two loops containing Tyr84 and Cys34 ... disulﬁdebonding pattern for the ﬁrst 50 residues in domain Idiffers from that of the other two domains. The ﬁrsttwo helices in the albumin sequence in domain I arenot tethered by disulﬁde bridges. Cys34...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... is also the reason why the determination of the spatial structure of the N-terminal domain con-taining the Cd(II)3-CysS9cluster was thus precluded.For a more detailed discussion of the dynamics ... The clusterstructure raises the possibility of a cooperative binding of the metal ions. Cooperative binding has beenreported but is not always found, being dependent on the type of metal, the ... ions by ninedeprotonated cysteines in a hexane-like cluster [M(II)3-Cys9], including three bridging and six terminal cyste-ines. The C-terminal a-domain (amino acids 31-61)binds four...

Báo cáo khoa học: N-Glycosylation is important for the correct intracellular localization of HFE and its ability to decrease cell surface transferrin binding pptx

... within the same cell. Furthermore, in cellsexpressing either the double or single mutants, wetended to see a decrease in the number of cells with the ability to reduce transferrin binding compared ... withbinding immunoglobulin protein. J Biol Chem 266,17648–17654.42 Williams A & Enns C (1993) A region of the C-terminalportion of the human transferrin receptor contains anasparagine-linked ... Levels of TfR2 are highest in hepatocytes, the predominant site of HFE expression, and recent stud-ies have conﬁrmed that the two proteins are capable of interacting [33,34]. The nature of these interactions...

Báo cáo khoa học: "Computational Lexical Semantics, Incrementality, and the So-called Punctuality of Events" potx

... glass of beer. In (9), the development of the drinking event can be measured along the subparts of the glass of beer. Each subpart of the incremental theme argument is mapped onto a subpart of ... associate with the CONST qualia role of chambre such a collection of instances of the movable_phys__obj type, the CONST role describing the meronymic constitution of a type. 502 In fact, the ability ... distinguished in this respect" verbs like eat are capable of m-incrementality, i.e., incrementality with individual-referring delimiting arguments (they have an incremental themes in the sense of...

Báo cáo khoa học: Common G102S polymorphism in chitotriosidase differentially affects activity towards 4-methylumbelliferyl substrates potx

... the speciﬁc activity of wild-type and Ser102 CHIT1 in media of COS-transfectedcells using silver-staining after gel electrophoresis. In their case, the protein signal staining intensities of aliquots ... located close to the binding cleft, we investigated whether possiblehydrogen-bonding interactions of the serine hydroxylmight result in altered substrate binding. Because the G102S mutation ... from that of wild-typeGly102 enzyme. Indeed, the structural analysesemployed show an increase in rigidity in the Ser102protein in a region associated with binding of the thirdsugar of 4MU–chitotrioside,...

Báo cáo khoa học: Kininogen-derived peptides for investigating the putative vasoactive properties of human cathepsins K and L docx

... L to participate in the production of kinins,using kininogens and synthetic peptides that mimic the insertion sites of bradykinin on human kininogens.Although both cathepsins processed high-molecular ... that cathepsin K is a new member of the kininase family. Both the kininogenase activity of cathepsin L and/or the kininase activity of cathepsin K maybe favored by the generation of an acidic ... 6–8. The kinin content (BKeq.) was measured by EIA, and the kinin values normalized as in Fig. 3.Fig. 5. Kininase activity of cathepsin K. Human cathepsin K wasincubated with bradykinin (enzyme/substrate...

Báo cáo khoa học: "DICTIONARY ORGANIZATION FOR MACHINE TRANSLATION: THE EXPERIENCE AND IMPLICATIONS OF THEUMIST JAPANESE PROJECT" ppt

... enabling the user to extend the available working vocabulary without bilingual knowledge. The dictionaries, the user, and the system The dictionary creation routines, whether in monolingual ... implementation of independently motivated linguistic-theoretic descriptions. The informing principles are those of modern 'lexicalist' unification-based linguistic theories: the English ... to the design of the parser. The dictionaries and the system The program form dictionaries factor out different types of information to be invoked at different stages in parsing and interpretation...

Tài liệu Báo cáo khoa học: Role of Kupffer cells in pathogenesis of sepsis-induced drug metabolizing dysfunction pptx

... mediator of lethal sepsis, triggering the production of proinﬂammatory cytokines and liverinjury [13].Therefore, the present study aimed to elucidate the role of KCs in the regulation of CYP isoform ... USA). Binding of all of the anti-bodies was detected using an ECL detection system (iNtRONBiotechnology, Seoul, Korea) in accordance with the manu-facturer’s instructions. The intensity of the ... [22]. ROS indirectly reduced the activity of selected isoforms of CYP by inducing phosphorylation of the isoforms [23]. Activated KCs cause oxidativestress on the surrounding tissue, releasing largeamounts...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... membraneproteins to migrate apically. Transcytosis of integrinswould therefore enable H. pylori with the intriguingpossibility of targeting the integrin b1receptor at api-cal membranes (Fig. 1). The ... signalling to activate the kinases FAK and Src [11], although mutation of the RGD-motif in CagL had no defect in T4SS functionssuch as phosphorylation of injected CagA during infec-tion in another ... Thus, the role of the CagL RGD-motif andCagY topology for injection of CagA is not yetresolved. It is also unclear why the effector proteinCagA itself can bind to the extracellular domain of inte-grin...

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