Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
Báo cáo khoa học: Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone pdf
... al. (Eur. J. Biochem. 271) Ó FEBS 2004 Differential regulation of fatty acid amide hydrolase promoter in human immune cells and neuronal cells by leptin and progesterone Mauro Maccarrone1,2, Valeria ... up -regulation of FAAH activity and expression in human T lymphocytes by Fig. 1. Effect of leptin a nd progesterone onFAAH activity and expression. (A ) Effect of leptin on the activity of FAAH in ... FAAH in human U937andCHP100cellsandontheproteincontent and the mRNA of FAAH in U937 cells. These cells were incubated for 24 h with leptin alone,or wit h 10 nM( 150 ngÆmL)1)leptininthepresence...
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Tài liệu Báo cáo khoa học: Down-regulation of reduced folate carrier may result in folate malabsorption across intestinal brush border membrane during experimental alcoholism docx
... counterstaining.Haematoxylin–eosin stainingHaematoxylin–eosin staining was carried out followingthe routine histological method described by Kayser and Bubenzer [38]. The haematoxylin–eosin staining techniqueemploys ... absorptive site of folic acid, i.e. the smallintestine.Expression of the RFC protein in BBM of theintestineThe effect of chronic alcoholism on the level of expres-sion of the RFC protein at the ... sec/mg protein)Fig. 2. [3H]Folic acid transport in intestinal BBMVs at differentintervals during the course of ethanol administration. An incubationbuffer of pH 5.5 and a [3H]folic acid concentration...
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Tài liệu Báo cáo khoa học: Differential effects of Mxi1-SRa and Mxi1-SRb in Myc antagonism ppt
... predominanttranscript in the adult intestine and in the developingembryo, whereas Mxi1-SRb transcripts predominate in the adult liver and kidney [12]. In our initial description of Mxi1-SRa and ... instead acti-vates this promoter (Fig. 4B).The finding of these differential functions is in linewith the dogma that the proteome gains functionalcomplexity by encoding multiple isoforms of ... Note that in (B) and (C), although 4¢,6-diamidino-2-phenylindole labels all of the cell nuclei (see also phase images),only some of the cells express the introduced Mxi1 proteins and, in these,...
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Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf
... changes in rota-tional coupling within the RPTPr ectodomain affectligand binding [43]. Our results suggest that oxidation-induced changes in the cytoplasmic domain may result in binding to ... werequenched by boiling for 5 min in reducingLaemmli sample buffer. Proteins were runon a 12.5% SDS-PAGE gel, blotted on PVDFmembrane and stained with Coomassie.Bands of interest (shown by arrows) ... depending on the exact location of theABCDEFFig. 2. Oxidation-induced conformationalchanges in the intracellular domains of RPTPs. GST-fusion proteins encoding theintracellular domain of (A)...
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Tài liệu Báo cáo khoa học: Differential expression of duplicated LDH-A genes during temperature acclimation of weatherfish Misgurnus fossilis Functional consequences for the enzyme ppt
... Molecu-lar cloning and cold-inducible gene expression of ferritinH subunit isoforms in rainbow trout cells. J Biol Chem271, 26908–26913.13 Tang SJ, Sun KH, Sun GH, Lin G, Lin WW & ChuangMJ ... affecting rates of transcription and ⁄ or transla-tion and ⁄ or mRNA and protein degradation. Thisrepresents a quantitative strategy to offset the lack orexcess of kinetic energy (as a measure of ... tele-osts, including weatherfish, zebrafish and others, tissue-specific light myosine chain forms are encoded by par-alogous genes mlc1 and mlc3, whereas in amphibians,birds and mammals these proteins...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... short interfering RNA (siRNA) in human cells. BothHO-2 mRNA and protein are expressed in the eight human cancer celllines examined, and HO-1 expression is detectable in five of the cell lines,including ... of HO-2mRNA and protein and induced the expression of HO-1 mRNA and protein. In contrast, the scrambledHO-2 siRNA did not affect the expression of HO-1 and HO-2 mRNAs and proteins in HeLa and ... as in Fig. 4.Expression levels of HO-1, HO-2 and GAPDH mRNAs were determined in HeLa cells (A) and HepG2 cells (C) by northern blot analysis.HO-1 and HO-2 proteins were determined in HeLa cells...
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Báo cáo khoa học: Differential recognition of heat shock elements by members of the heat shock transcription factor family ppt
... protein) of YYY49 and YYT42 con-tained approximately 1 and 2 ng of hHSF2, and those of YYT50 and YYT17 contained approximately 0.01 and 0.1 ng of hHSF4, asjudged by the intensity of each band. ... proteins, and increased accumulation of HSPs is essential for sur-vival of cells exposed to protein-damaging stresses,including heat shock. The structure of HSF comprisesa conserved DNA-binding ... times.Extracts of cells expressing hHSF2 (2 lg of protein) or hHSF4(20 lg of protein) and recombinant hHSF proteins (not shown)were subjected to immunoblot analysis with antibodies againsthHSF2 and hHSF4....
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Báo cáo khoa học: Versatile regulation of multisite protein phosphorylation by the order of phosphate processing and protein–protein interactions pptx
... obtained in special cases. The order of phos-phate processing and the characteristics of protein–protein interactionsturn out to be of overriding importance for both sensitivity and speed of response. ... sameprotein, may be useful in testing our theoretical con-clusions. The integration of experimental informationwith modeling approaches will certainly provide newinsights into the intramolecular and ... attainhigher concentrations in the case of random and cyclicphosphate processing than in sequential processing(Fig. 3D). To illustrate the effect of the kinetic designon the differential regulation...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... blockedinactivation of the AMPK by insulin (Fig. 7), suggesting adominance of the fatty acid- driven pathway for activation of AMPK over at least some aspects of insulin signalling.This dominance of ... activation of AMPK by fatty acids and insulin and adrenergic signalling processesFig. 5 shows studies focused on the dominant a-2 AMPKisoform. Insulin decreased a-2 AMPK activity by 55% in the ... characteristic of the fasting state. The inclusion of fatty acids resulted in increased activities of the a-1 or the a-2 isoforms of AMP-activated protein kinase (AMPK), increased phosphoryla-tion of acetyl-CoA...
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Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx
... fibrils.Thus, interactions between the collagens and decorin are of paramount importance in attaining and maintainingtissue integrity. In the present study we investigate the binding properties of wild-type ... c ylinder. (A) Interaction involving d imerization of decorin and binding of both collagens to the i nner surface of decorin. (B) Inter-action of type I collagen with t he inner surface of the ... amino acid E180 may b e not only important for the binding of decorinto type I collagen, but may a lso be involved in t he binding totype VI. The interaction of type VI collagen with decorinhas...
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