Báo cáo khoa học: The Thermoplasma acidophilum Lon protease has a Ser-Lys dyad active site pot

... wild-type Lon the protease domain might influ- ence the activity of the ATPase domain. Keywords:AAA + protease; archaea; Lon( La) endopepti- dase; Lon (La) protease; Ser-Lys dyad. Endopeptidase La (EC ... [21] (Fig. 1 A, B). TaLon encompasses an N-terminal ATPase associated with various cellular a ctivities ( AAA + domain) and a C-terminal protease domain, but lacks...

Ngày tải lên: 30/03/2014, 15:20

... in RNA helicase A (RHA), which has a DEXH helicase domain for the unwinding of duplex of nucleic acids [11], and in RNaseIII and the related Dicer ⁄ Drosha enzymes of the RNAi ⁄ miRNA pathway, ... which carry an RNase domain for dsRNA cleavage and pro- cessing [12–14]. A second group of dsRBM-containing proteins do not carry an identifiable catalytic domain. Examples are the st...

Ngày tải lên: 19/02/2014, 17:20

... ABSTRACT Model-theoretic semantics provides a computationally attractive means of representing the semantics of natural language. However, the models used in this formalism are static and are ... management of the relationship is external to the model. This mechanism has the disadvantage that it involves high overhead - the denotational relationships must be repeatedly...

Ngày tải lên: 21/02/2014, 20:20

... ATCCTTTATGGTGAGATCAGCTTCAATATCCGTCCA Vps4–TRP F TTAAAGGCTATCAAATCGCAAGAACAGTTCACTAGA Vps4–TRP R TCTAGTGAACTGTTCTTGCGATTTGATAGCCTTTAA Vps4–RDF F GAAGCAAGAACAGTTCACTTAGTCAATTGATTAACGTG Vps4–RDF R CACGTTAATCAATTGACTAAGTGAACTGTTCTTGCTTC Table ... the AAA superfamily typically contain one or two ATPase domains that assemble into one or two stacked hexameric rings. The ATPase catalytic site i...

Ngày tải lên: 07/03/2014, 05:20

... and XhoI sites at either end, using the following primers: forward, 5¢-CGCGGATCCTGGTCTTTCCAAAATATTC AGGCCA-3¢ and reverse, 5¢-GTCCTCGAGGTACATCA CATCTCTGAT-3¢. After digestion, the PCR-amplified fragment ... levels after uptake of the eggs, but that its levels drop off after the larvae are hatched and as the juvenile seahorses are preparing to leave the pouch. This suggests the pa...

Ngày tải lên: 07/03/2014, 17:20

... accessible areas were calculated with the program naccess [56]. Atomic coordinates and the X-ray amplitudes of the RNase A IMP, and RNase A AMP, complexes have been deposited in Research Collaboratory for ... 3989 has been attributed to the lattice contacts that limit access to the active site of mol B in the asymmetric unit. In all free RNase A structures reported so fa...

Ngày tải lên: 07/03/2014, 21:20

... demonstrated that MtCCO mediates the symmetrical cleavage of b-carotene at the C15-C15¢ site, as well as the asymmetrical cleavage of the C13-C14 or the C13¢-C14¢ double bond. To test the cleavage ... source of apocarotenoids. In mam- mals, the synthesis of apocarotenoids, including retinoic acid, is initiated by the b-carotene cleavage oxygenases I and II catalyzing either...

Ngày tải lên: 15/03/2014, 23:20

... cyclase by the atrial natriuretic factor. FEBS Lett 219, 375–379. 21 Marala RB, Sitaramayya A & Sharma RK (1991) Dual regulation of atrial natriuretic factor-dependent guanylate cyclase activity ... Sharma RK (2002) Evolution of the membrane guany- late cyclase transduction system. Mol Cell Biochem 230, 3–30. 40 Yamazaki A, Yu H, Yamazaki M, Honkawa H, Matsu- ura I, Usukura J &...

Ngày tải lên: 16/03/2014, 23:20

... secreted early after monocyte activation and HMGB1 at a later stage. IL-1b consistently acts at an early phase of inflam- mation whereas HMGB1 functions as a late mediator of inflammation (see above). These ... proteins in mammalian cells revealed the existence of a machinery that is capable of translocating the protein across the plasma membrane [103], demonstrating a conserved p...

Ngày tải lên: 17/03/2014, 03:20

... that directly upstream of P HOP2 (5¢-ATACAATTAATTGACATCAGCAGACAGCAAAT GCACTTGATATACGCAGCTCGACTACGTCGTAAG GCCG-3¢ and 5¢ -ATCTTTCAAATAGAGCCTGG-3¢). The amplified DNA fragments were used to transform BFG2Z18-K3 5A, ... 5¢-AAATATAAAACGCTAGCGTCGACATGGC GC-3¢ and 5¢-AGCGTAAAGGATGGGGAAAG-3 ¢. The final ratio of target cells was determined by the number of colonies retaining the target gene di...

Ngày tải lên: 22/03/2014, 21:20