Báo cáo khoa học: Regulation of the expression and subcellular localization of the taurine transporter TauT in mouse NIH3T3 fibroblasts doc

... within the initial 20 min following the addition of 14 C-labelled taurine. Using taurine uptake within the initial 20 min in NaCl medium containing extracellular taurine (0–50 l M ) and fitting the ... specific activity and the protein content. The taurine in ux (nmolÆ gprotein )1 Æmin )1 ) w as estimated as the slop e o f the cellular taurine uptake plotted vs...

Ngày tải lên: 30/03/2014, 15:20

... in x-5a gene in Fig. 2, includes all of the detected IgE-binding epitope sequences. After amplification of the DNA encoding this half of the x-5a gliadin protein by PCR, the DNA fragment was inserted ... recombinant x-5 gliadin protein that can be used for diagnosis and possibly treatment of WDEIA. Results Identification of IgE-binding epitopes in x-5 gliadin The IgE...

Ngày tải lên: 20/02/2014, 01:20

... maintained in the apo-form of the protein [43] and so are especially interesting for the study of the folding mechanism, intermediates and stability of the protein by CD spectra. Usually, the ... using the program X - PLOR . The program TURBO - FRODO was used to fit the side chains of Tyr35 and Val61, and then the model was adjusted manually to improve th...

Ngày tải lên: 08/03/2014, 10:20

... residues belonging to the loop (Trp74 and Met75) are involved in DNA binding [16]. It is clear that the arm of MC1 is essential for DNA binding and bend- ing. The interaction mode of MC1 is probably ... b-ribbon arms wrap around the minor groove of the DNA and, at the tip of each arm, the conserved Pro intercalates between base pairs, creating and ⁄ or stabilizi...

Ngày tải lên: 22/03/2014, 17:20

... recognized the amino-terminal Ala23–Val28 loop of L-PGDS, revealed that both the mRNA and the intact form of L-PGDS were local- ized in the cells of Henle’s loop and the glomeruli of the kidney, indicating that ... representation of GST-fusion proteins containing a series of amino-terminus-truncated human L-PGDS. The amino-terminal amino acid residue of each...

Ngày tải lên: 30/03/2014, 01:20

... stronger binding of TRPV1 to the plus end rather than the minus end of A B Fig. 1. Characteristic of the tubulin-binding motifs located at the C-terminus of TRPV1. (A) The extreme C-terminus of both ... Ca 2+ . In recent years, TRPV1 has gained extensive attention for its involvement in signalling events in the context of pain and other pathophysiological con...

Ngày tải lên: 30/03/2014, 10:20

... concep- tion of transformation (Harris, 1957). In this view, the original verbatim string is the surface form of the sentence, and as in linguistic theory should be constrained in some manner ... Proceedings of the 47th Annual Meeting of the ACL and the 4th IJCNLP of the AFNLP, pages 746–754, Suntec, Singapore, 2-7 August 2009. c 2009 ACL and AFNLP What li...

Ngày tải lên: 20/02/2014, 07:20

... purpose of utteranc- es and their domain context, it is then possible to correct some of the insertion and mis-recognised word errors from the SR and determine the com- municative intent of the ... rules of the form A -> B C to the form A -> B Link C, and add con- straints to the rules application in terms of the value of the =breaking indi...

Ngày tải lên: 20/02/2014, 21:20

... below. Viral binding to filopodia Although the virus binding to cells results from the interaction of gC and ⁄ or gB with heparan sulfate (HS), the location on the cell where this binding can occur the ... receptors. These include B5 and avb3 integrins [39,40]. Expression cloning in porcine kidney cells resistant for HSV-1 entry has led to the discovery of B5, a type-2...

Ngày tải lên: 23/03/2014, 04:20

... tau and tubulin-tau complex using dithiothreitol-induced insulin aggregation assay (Fig. 2A). Here, curve 1 is insulin alone and in the presence of 1 : 4 ratio (w/w) of insulin to tubulin the inhibition ... proteins, it is physio- logically relevant to determine in vitro if the binding of MAPs to the C-terminal region of tubulin adversely affects the chaperone-like f...

Ngày tải lên: 23/03/2014, 12:20