Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx

Tài liệu Báo cáo khoa học: FOXM1c transactivates the human c-mycpromoter directly via the two TATA boxes P1 and P2 docx

... number of coloniesnumber of colonies in %+Engr-ER-myc 0,9 310 7Engr-ER-myc- 1, 09 91 FOXM1c (18 9-566)-Engr-2,8635,5FOXM1c (18 9-587; 744-762)- 1, 0694FOXM1c (18 9-762)- 1, 0694- 11 00control+ 11 00controlGal-Engr- 1, 1686FOXM1c (18 9-743)-Engr-2,9234,876 218 976 218 9744587 18 9743Engr-RPDGALEngr-RPDEngr-RPDER-HBD566 18 9Engr-RPDFig. ... pmyc(-224/ -13 6)luc c FOXM1c (18 9-762) 11 0 2 4 6 8 10 12 14 16 1, 1 1, 6 y t i v i t c a e s a r e f i c u l e v i t a l e r pmycluc c FOXM1c (18 9-762) 11 0 10 20 30 ... ;785-98 1 (c1MX OF rgnE-)665-98 1( c1MX OF 12 5 10 0502575DFOXM1cHL-600 h 29 hFOXM1c (18 9-743)-EngrFOXM1c (18 9-762)FOXM1c (18 9-587;744-762)FOXM1c (18 9-566)-Engr 10 μgCOHTconstructaveragefold reductionof...

Báo cáo khóa học: Acharan sulfate, the new glycosaminoglycan fromAchatina fulica Bowdich 1822 Structural heterogeneity, metabolic labeling and localization in the body, mucus and the organic shell matrix docx

... forms of acharan sulfate are presentin the soft body, mucus and the organic shell matrix The GAG component of the soft body, mucus and the organic shell matrix of the giant African snail was isolatedÓ ... Proteus vulgaris.(Received 17 November 2003, accepted 12 January 2004)Eur. J. Biochem. 2 71, 845–854 (2004) Ó FEBS 2004 doi :10 .11 11/ j .14 32 -10 33.2004.03989.xinhibition of vascular endothelial growth ... repro-duction and protection of epithelial surfaces [12 ].Achatina fulica is an intermediate host to Angiostrongyluscantonensis [13 ], the etiological agent of meningoencephalicangiostrongiliasis [14 ], and...

Tài liệu Báo cáo khoa học: TMPRSS13, a type II transmembrane serine protease, is inhibited by hepatocyte growth factor activator inhibitor type 1 and activates pro-hepatocyte growth factor pdf

... ...SPK1 K2TM0 10 2030405060708090 10 00. 01 0 .1 1 10 10 0 10 00Fig. 2. Dose dependence of the inhibitory activity of solubleforms of HAI -1 and HAI-2 against the protease activity of TMPRSS13. ... Schematic representation of the structure of the full-length HAI -1 (1) and soluble forms of HAI -1, HAI -1 NK1LK2(2) and HAI -1 NK1 (3), tagged at the C-terminus with myc-His.SP, signal peptide; ... different: the inhibition curve of HAI-2 was sigmoidal, whereas that of HAI -1 NK1was not (Fig. 2C).Formation of complexes of TMPRSS13 and HAI -1 NK1To conﬁrm the inhibitory effect of HAI -1 NK1 onTMPRSS13,...

Báo cáo khoa học: Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment potx

... Internalization of the human CRF receptor 1 is independent of classical phosphorylation sites and of b-arrestin 1 recruitment Trine N. Rasmussen 1, 3, Ivana Novak3 and Søren M. Nielsen2 1 Department ... neuro-peptide ﬁrst isolated in 19 81 [1] as the main stimulator of the release of adenocorticotropin from the pituitary. By activa-tion of CRF Receptor 1 (CRFR1) [2], CRF regulates notonly the endocrine, ... phosphorylation and recruitment of b-arrestin might be part of an event separate from that of CRFR1 internalization. Agonist-mediated internalization of CRFR1 was dem-onstrated by visualizing CRFR1–EGFP...

Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

... revised 5 May2006, accepted 15 May 2006)doi :10 .11 11/ j .17 42-4658.2006.05 319 .xHeme oxygenase consists of two structurally related isozymes, heme oxyge-nase -1 and and heme oxygenase-2, each of ... analysis.Opposite effects of hypoxia on expression of HO -1 and HO-2 mRNA in YN -1 cellsTo explore the mechanism of the hypoxia-mediatedreduction of HO -1 and HO-2 protein levels, we ana-lyzed the ... car-bon monoxide and biliverdin at the expense of molecu-lar oxygen and NADPH [1, 2]. HO consists of twostructurally related isozymes, HO -1 and HO-2 [3–5].Characteristically, human HO -1 contains...

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate speciﬁcity by replacement of Asn149 with Asp docx

... kcat(s -1 ) KmArg(mM) kcat⁄ KmArg(M -1 Æs -1 )Wild-type 249 ± 10 1. 4 ± 0 .1 177.9His120Asn 13 1 ± 8 1. 6 ± 0 .1 81. 9His145Asn 238 ± 12 1. 5 ± 0 .1 158.6Asn149Asp ND ND NDFig. 1. Fluorescence ... consequence of the mutagenic replacements.Effects of the His120Asn and His145Asnmutations on the afﬁnity of metal bindingto arginase IITo further examine the effects of the His120ﬁAsn and His145ﬁAsn ... the transition state analog S-(2-boro-noethyl)-l-cysteine (BEC). His120 and His145, and the corresponding His1 01 and His126 in arginase I, weredescribed among the ligands for coordination of Mn2+Aand...

Báo cáo khoa học: Insulin resistance in human adipocytes occurs downstream of IRS1 after surgical cell isolation but at the 1 level of phosphorylation of IRS1 in type 2 diabetes pot

... 1. 1 1. 8 1. 1 1. 8 1. 1 1. 8IRS1 0.6–0.7 0.6–0.7 1. 8–2.0 1. 8–2.0 1. 8–2.0 1. 8–2.0PKB 0.9 1. 1 0.3–0.4 0.6–0.7 0.6–0.7 0.6–0.7 0.6–0.7Glucose transport 0 .1 0.2 0.02–0.03 0 .1 0.2 0 .1 0.2 0 .1 0.2 0 .1 0.2A. ... the dose–response curves in Figs 2,4, and 7.AnalysisSubjectsNormal Female diabetic Male diabeticDirectly o ⁄ n Directly o ⁄ n Directly o ⁄ nInsulin receptor 1. 1 1. 8 1. 1 1. 8 1. 1 1. 8 1. 1 1. 8 ... resistance in human adipocytesFEBS Journal 272 (2005) 14 1 15 1 ª 2004 FEBS 15 1Insulin resistance in human adipocytes occursdownstream of IRS1 after surgical cell isolation but at the level of...

Báo cáo khoa học: Internalization of cystatin C in human cell lines docx

... ﬂowcytometry and the median of the ﬂuorescence of the cell populationwas calculated, corrected for the control value and then related to the degree of labelling of the protein used. Dashes and rings ... & Lee AG (19 79)Thermostable endogenous inhibitors of cathepsins B and H. Eur J Biochem 10 1, 15 3 16 1.Supporting information The following supplementary material is available:Fig. S1. Confocal ... Overexpression of cysta-tin C has been shown to alter the metastatic properties of B16F10 melanoma cells [9] and to inhibit the motility and in vitro invasiveness of B16F10 [10 ] and SCC-VIIsquamous...

Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx

... intermediatefor the coupled hydroxylation of the cosubstrates [ 21, 41] .However, the sequence of binding of cofactor and substrate is still a matter of discussion and may be diﬀerent comparingwild-type and ... ana-lysis [11 ] and modulation of Ser16 phosphorylation by PKA [12 ]. The binding studies by Shiman and collaborators [11 ,15 ] were interpreted to support a working model thatincludes three types of ... that the distances from Ser23O to O1¢ and O2¢are slightly different (Table 1) because of the differentpositions and hybridizations of the C6 atom of the pyrazinering in the two redox states [18 ,20]....

Báo cáo khoa học: Expressed as the sole Hsp90 of yeast, the a and b isoforms of human Hsp90 differ with regard to their capacities for activation of certain client proteins, whereas only Hsp90b generates sensitivity to the Hsp90 inhibitor radicicol pdf

... inresponse to Mkk1 ⁄ 2-directed phosphorylation of the MAP kinase activation loop [3 ,14 ,18 ], Y2H interaction of these MAP kinases with the two isoforms of human Hsp90 was strengthened by heat shock ... sensitivity is normally associated with compromised activity of the Hsf1p CT domain [14 ,22], the compromised heatactivation of this domain with Hsp90b present in the yeast (Fig. 1C) is a plausible ... April 2007, revised 1 June2007, accepted 3 July 2007)doi :10 .11 11/ j .17 42-4658.2007.05974.xHeat shock protein 90 (Hsp90) is a molecular chaperone required for the activity of many of the most important...

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