Báo cáo khoa học: Escherichia coli cyclophilin B binds a hig

Báo cáo khoa học: Escherichia coli cyclophilin B binds a highly distorted form of trans-prolyl peptide isomer doc

... molecules B bound to Suc-Ala-trans-Pro- Ala-pNA and Ac-Ala-Ala-trans-Pro-Ala-AMC (Figs 4 and 7A) , the N g2 atom of Arg48 forms a hydrogen bond with the distance of 2.62 A ˚ and 2.72 A ˚ to CO of trans-Pro ... which peptides having a trans- form proline, i.e. succinyl-Ala-trans-Pro-Ala-p-nitroanilide and acetyl-Ala-Ala-trans-Pro-Ala-amidomethylcoumarin, are bound on a K16...

Ngày tải lên : 30/03/2014, 15:20

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Báo cáo khoa học: Escherichia coli cyclopropane fatty acid synthase Mechanistic and site-directed mutagenetic studies potx

... from Promega, and the cfa gene was ampliﬁed using Taq DNA polymerase (Promega) and the following two primers: 5¢-CGCGAATTCAGGAGGATTTTATGCACCACCA CCACCACCACAGTTCATCGTGTATAGAAGAA-3¢ containing an EcoRI ... has R 1 S R 2 CH 3 CH 3 CH 3 Enz-Nu H H C H H H R 1 S R 2 H 3 C Ylide R 1 S R 2 CH 2 OR Enz–Base Enz–BaseH Enz–Nu Enz–Nu A B Enz Metal Metal CH 2 Enz–Nu Enz–Base Enz–Base Carbenoid H H Sc...

Ngày tải lên : 07/03/2014, 16:20

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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... implications. Abbreviations Ab, b- amyloid; AD, Alzheimer’s disease; apo-T, apo-thionein; DTNB, 5,5¢-dithiobis(2-nitrobenzoic acid)); GIF, neuronal growth-inhibitory factor; M(II), divalent metal ... but retained the two ligands. By contrast, the slow-reacting cisplatin had all ligands replaced with thiolates. Cisplatin binds preferentially to the b- domain (but binding with transplatin h...

Ngày tải lên : 16/02/2014, 15:20

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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... determined by SDS/PAGE, was 10 kDa. A similar result (8.8 kDa) was obtained by mass spectrometry (MALDI-TOF) analysis (Fig. 2). A twice as large value (18 kDa) was found by gel-permeation chromatography ... molecular masses (kDa). Lane A: low molecular mass standards; lane B: crude extract; lane C: after anion exchange chromatography on a DE 52-cellulose column; lane D: after anion ex...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khoa học: ˚ cDNA cloning and 1.75 A crystal structure determination of PPL2, an endochitinase and N-acetylglucosaminebinding hemagglutinin from Parkia platycephala seeds potx

... BioMol-Laboratory, Departamento de Bioquı ´ mica e Biologia Molecular, Universidade Federal do Ceara ´ , Fortaleza, Ceara ´ , Brazil 2 Departamento de Fı ´ sica, Universidade Estadual Paulista, UNESP, ... Brazil 9 Laboratorio de Bioquı ´ mica Marinha, Departamento de Engenharia de Pesca, Universidade Federal do Ceara ´ , Fortaleza, Ceara ´ , Brazil Keywords endochitinase; glycosyl hydrolase f...

Ngày tải lên : 16/03/2014, 13:20

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Báo cáo khoa học: "Robust Approach to Abbreviating Terms: A Discriminative Latent Variable Model with Global Information" docx

... Japan). References Eytan Adar. 2004. SaRAD: A simple and robust ab- breviation dictionary. Bioinformatics, 20(4):527– 533. Hiroko Ao and Toshihisa Takagi. 2005. ALICE: An algorithm to extract abbreviations ... case of the DPLVM, with only one latent variable assigned for each label. The DPLVM uses latent variables to capture additional information that may not be ex- pressed by the observ...

Ngày tải lên : 17/03/2014, 01:20

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Báo cáo khóa học: High level cell-free expression and speciﬁc labeling of integral membrane proteins doc

... ctc tgc ttt cat taa aac YﬁK-up cgg cat atg aca ccg acc ctt tta agt gct ttt tgg YﬁK-low cgg aag ctt tta ata gaa aat gcg tac cgc gca ata gac EmrE-upNh cgg gct agc aac cct tat att tat ctt ggt gg EmrE-lowNh ... acc EmrE-upNd cgg cat atg aac cct tat att tat ctt ggt ggt gc EmrE-low cgg aag ctt tta atg tgg tgt gct tcg tga c TehA-up cgg cat atg cag agc gat aaa gtg ctc aat ttg TehA-low cgg aag ctt tta...

Ngày tải lên : 30/03/2014, 13:20

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Báo cáo khoa học: Direct CIII–HﬂB interaction is responsible for the inhibition of the HﬂB (FtsH)-mediated proteolysis of Escherichia coli r32 by kCIII docx

... CIII–H B interaction is responsible for the inhibition of the H B (FtsH)-mediated proteolysis of Escherichia < /b> coli < /b> r 32 by kCIII Sabyasachi Halder 1 , Subhamoy Banerjee 1,2 and Pradeep Parrack 1 1 ... CIII protein of bacteriophage lambda exhibits antiproteolytic activity against the ubiquitous metalloprotease H B (FtsH) of Escherichia < /b> coli,< /b> thereby stabil...

Ngày tải lên : 23/03/2014, 10:20

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Báo cáo khoa học: Saccharomyces cerevisiae Cox18 complements the essential Sec-independent function of Escherichia coli YidC doc

... Cox18 can be considered a bona ﬁde member of the YidC ⁄ Oxa1 ⁄ Alb3 family. Results Properties of YidC, Oxa1 and Cox18 Members of the YidC ⁄ Oxa1 ⁄ Alb3 family are charac- terized by a conserved ... ligase and alkaline phosphatase were obtained from Invi- trogen (Carlsbad, CA, USA). Pansorbin was purchased from Merck (Darmstadt, Germany). Megashort script T7 tran- scription kit was...

Ngày tải lên : 30/03/2014, 03:20

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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf

... CD, Crabbe T, Okkenhaug K, Vanhaesebroeck B, Turner M, Webb L, Wymann MP et al. (2005) Sequential acti- vation of class IB and class IA PI3K is important for the primed respiratory burst of human but ... International, Camarillo, CA, USA) to detect active Akt kinase, as well as a separate Akt polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA) to determine total Akt, as d...

Ngày tải lên : 18/02/2014, 16:20

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