Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc

... Structured DNA promotes phosphorylation of p53 by DNA- dependent protein kinase at serine 9 and threonine 18 Se ´ bastien Soubeyrand 1 , Caroline Schild-Poulter 1 and Robert J. G. ... of p53 Wt or p53 S9A/T18V phosphorylation by DNA- PK . DNA- PK was incubated with the indicated p53 species in the presence of 50 l M ATP and assessed for total...

Ngày tải lên: 30/03/2014, 15:20

... regulation of DNA replication. However, detailed characterization of the phosphorylation of MCM4 remains to be per- formed. We examined the phosphorylation of human MCM4 at Ser3, Thr7, Thr 19, Ser32, ... MCM4 phosphorylation on chromatin at these sites began to decrease during the late S phase and was greatly reduced in the G 2 phase. MCM4 33 39 P-7 P- 19 P-32 P-110 33...

Ngày tải lên: 16/03/2014, 13:20

... methods Nucleic acids A 180 bp dsDNA fragment was obtained by PCR amplifi- cation of pUC18 dsDNA, using the primers 180 L and 180 R (Table S1). Closed-circular pUC18 dsDNA and M13mp18 ssDNA were purchased from ... (Fuji Film). Detection of possible endonuclease- and topoisomerase activities and covalent attachment of protein at DNA termini TF-AtSPO11-1 (0.5 lm) was inc...

Ngày tải lên: 29/03/2014, 09:20

... identity with that of Bacil- lus amyloliquefaciens TS-Phy (YP 090 097 ), and a C-ter- minal domain (PhyH-DII, residues 3 19 644) that has 49% identity with that of TS-Phy (YP 090 097 ) [15] and 41% with ... Environmental factors that affect the catalytic activities of PhyH and PhyH-DII. (A) Effect of 0–5.0 mM Ca 2+ at 37 °C. (B) Effect of pH at 37 °C. (C) Effect of temp...

Ngày tải lên: 14/02/2014, 15:20

... b-defensin-mediated signals positively regu- late the expression of matrix metalloproteinase genes and negatively regulate that of tissue inhibitor of matrix metalloproteinase genes, thus modulating tissue repair ... that b-defensins participate in the triggering of an immune response and in the natural process of elimination of activated antigen-presenting cells and termina...

Ngày tải lên: 18/02/2014, 06:20

... water, thus, supporting the formation of sodiated LMWC when alkali was added. The drawbacks of using acetonitrile were, its high cost and flash treatment of the supernatant at elevated temperature, ... products. An examination of DA values indicates that the total DA of the products is equal to that of native chitosan ( 19% for LMWC and 31.2% for chito-oligomers + monomer, th...

Ngày tải lên: 19/02/2014, 12:20

... produce both detailed and schematic plots of protein structures. J Appl Crystallogr 24, 94 6 95 0. M. Wendeler et al. Enzyme-binding region of GM2-activator protein FEBS Journal 273 (2006) 98 2 99 1 ª 2006 The ... U & Sandhoff K (2003) Expression of recombinant human GM2-activator protein in insect cells: purification and characterization by mass spectrometry. Prot Expr...

Ngày tải lên: 07/03/2014, 12:20

... & Virshup DM ( 199 9) Regulation of beta-catenin signal- ing by the B56 subunit of protein phosphatase 2A. Science 283, 20 89 2 091 . 79 Grosschedl R, Giese K & Pagel J ( 199 4) HMG domain proteins: ... 357–360 [1 79] 29 190 0 ⁄ 40 34 ⁄ 0 2 39 164 ⁄ 8 Mdm2, ATM, ERK, p38, BCL-X L [180 ] Mdm2 (Q0 098 7) 1–17 [181 ]; 17–24, 110– 125 [182 ]; 210–304 [183 ] 26 95 ⁄ 411⁄ 07...

Ngày tải lên: 07/03/2014, 21:20

... SipA polymerizes actin by stapling filaments with non- globular protein arms. Science 301, 191 8– 192 1. 19 Hayward RD & Koronakis V ( 199 9) Direct nucleation and bundling of actin by the SipC protein of invasive Salmonella . ... ( 199 9) Evolution and mechanism from structures of an ADP-ribosylating toxin and NAD complex. Nat Struct Biol 6, 93 2 93 6. 41 Considine...

Ngày tải lên: 14/03/2014, 22:20

... and denatured states in the absence of denaturant and b N and b D are the slopes of the native and denatured baselines and [D] is the concentration of denaturant. F ¼ ða N þb N ½DÞþðða D þb D ½DÞexpððm DÀN ð½DÀ½D 50% ÞÞÞ=RT 1þexpðm DÀN ð½DÀ½D 50% ÞÞ=RT ð3Þ The ... Barkley MD ( 199 8) Toward understanding tryptophan fluorescence in proteins. Biochemistry 37, 99 76 99 82....

Ngày tải lên: 16/03/2014, 01:20