Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc
Báo cáo khoa học: Structured DNA promotes phosphorylation of p53 by DNA-dependent protein kinase at serine 9 and threonine 18 doc
... Structured DNA promotes phosphorylation of p53 by DNA- dependent
protein kinase at serine 9 and threonine 18
Se
´
bastien Soubeyrand
1
, Caroline Schild-Poulter
1
and Robert J. G. ... of p53
Wt
or p53
S9A/T18V
phosphorylation by DNA- PK . DNA- PK was incubated with the indicated p53 species in the presence of 50 l
M
ATP and assessed for total...
Báo cáo khoa học: Site-specific phosphorylation of MCM4 during the cell cycle in mammalian cells pot
... regulation of DNA replication. However, detailed
characterization of the phosphorylation of MCM4 remains to be per-
formed. We examined the phosphorylation of human MCM4 at Ser3,
Thr7, Thr 19, Ser32, ... MCM4 phosphorylation on
chromatin at these sites began to decrease during the
late S phase and was greatly reduced in the G
2
phase.
MCM4
33 39
P-7 P- 19 P-32 P-110
33...
Báo cáo khoa học: A DNA-binding surface of SPO11-1, an Arabidopsis SPO11 orthologue required for normal meiosis docx
... methods
Nucleic acids
A 180 bp dsDNA fragment was obtained by PCR amplifi-
cation of pUC18 dsDNA, using the primers 180 L and
180 R (Table S1). Closed-circular pUC18 dsDNA and
M13mp18 ssDNA were purchased from ... (Fuji Film).
Detection of possible endonuclease- and
topoisomerase activities and covalent
attachment of protein at DNA termini
TF-AtSPO11-1 (0.5 lm) was inc...
Tài liệu Báo cáo khoa học: The tandemly repeated domains of a b-propeller phytase act synergistically to increase catalytic efficiency doc
... identity with that of Bacil-
lus amyloliquefaciens TS-Phy (YP 090 097 ), and a C-ter-
minal domain (PhyH-DII, residues 3 19 644) that has
49% identity with that of TS-Phy (YP 090 097 ) [15] and
41% with ... Environmental factors that affect the catalytic activities of PhyH and PhyH-DII. (A) Effect of 0–5.0 mM Ca
2+
at 37 °C. (B) Effect of pH
at 37 °C. (C) Effect of temp...
Tài liệu Báo cáo khoa học: Multifunctional host defense peptides: Antimicrobial peptides, the small yet big players in innate and adaptive immunity docx
... b-defensin-mediated signals positively regu-
late the expression of matrix metalloproteinase genes
and negatively regulate that of tissue inhibitor of
matrix metalloproteinase genes, thus modulating tissue
repair ... that
b-defensins participate in the triggering of an immune
response and in the natural process of elimination of
activated antigen-presenting cells and termina...
Tài liệu Báo cáo khóa học: Non-specific depolymerization of chitosan by pronase and characterization of the resultant products pptx
... water, thus,
supporting the formation of sodiated LMWC when alkali
was added. The drawbacks of using acetonitrile were, its
high cost and flash treatment of the supernatant at elevated
temperature, ... products. An examination of DA values indicates that
the total DA of the products is equal to that of native
chitosan ( 19% for LMWC and 31.2% for chito-oligomers
+ monomer, th...
Báo cáo khoa học: The enzyme-binding region of human GM2-activator protein pdf
... produce
both detailed and schematic plots of protein structures.
J Appl Crystallogr 24, 94 6 95 0.
M. Wendeler et al. Enzyme-binding region of GM2-activator protein
FEBS Journal 273 (2006) 98 2 99 1 ª 2006 The ... U & Sandhoff K (2003)
Expression of recombinant human GM2-activator
protein in insect cells: purification and characterization
by mass spectrometry. Prot Expr...
Báo cáo khoa học: Flexible nets The roles of intrinsic disorder in protein interaction networks potx
... &
Virshup DM ( 199 9) Regulation of beta-catenin signal-
ing by the B56 subunit of protein phosphatase 2A.
Science 283, 20 89 2 091 .
79 Grosschedl R, Giese K & Pagel J ( 199 4) HMG domain
proteins: ... 357–360 [1 79]
29 190 0 ⁄ 40 34 ⁄ 0 2 39 164 ⁄ 8 Mdm2, ATM, ERK, p38, BCL-X
L
[180 ]
Mdm2 (Q0 098 7) 1–17 [181 ]; 17–24, 110– 125 [182 ];
210–304 [183 ]
26 95 ⁄ 411⁄ 07...
Báo cáo khoa học: Actin as target for modification by bacterial protein toxins docx
... SipA
polymerizes actin by stapling filaments with non-
globular protein arms. Science 301, 191 8– 192 1.
19 Hayward RD & Koronakis V ( 199 9) Direct nucleation
and bundling of actin by the SipC protein of invasive
Salmonella
. ... ( 199 9) Evolution and mechanism from structures of
an ADP-ribosylating toxin and NAD complex. Nat
Struct Biol 6, 93 2 93 6.
41 Considine...
Báo cáo khoa học: Untangling the folding mechanism of the 52-knotted protein UCH-L3 potx
... and denatured states in the absence
of denaturant and b
N
and b
D
are the slopes of the native
and denatured baselines and [D] is the concentration of
denaturant.
F ¼
ða
N
þb
N
½DÞþðða
D
þb
D
½DÞexpððm
DÀN
ð½DÀ½D
50%
ÞÞÞ=RT
1þexpðm
DÀN
ð½DÀ½D
50%
ÞÞ=RT
ð3Þ
The ... Barkley MD ( 199 8) Toward understanding
tryptophan fluorescence in proteins. Biochemistry 37,
99 76 99 82....