Báo cáo khoa học: Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase docx

... Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase Michael Koch 1 , Constanze Breithaupt 1 , ... enzyme complex. This relates to the high er dissociation constant in the riboflavin mutant complex compared t o the riboflavin wild-type complex. Ó FEBS 2004 Structural basis of charge trans...

Ngày tải lên: 30/03/2014, 15:20

... of p63a is found to undergo a number of mutations. A number of point mutations have been identified in exons 13 and 14 of the p63 gene. These mutations can be classified into groups according to ... fluorescence of W598 as the probe of measurement. The measure of stability of these pro- teins was determined by the amount of the denaturant required to get 50% of the pr...

Ngày tải lên: 22/03/2014, 16:20

... C ˇ eske ´ Bude ˇjovice; 3 Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences of the Czech Republic, Tr ˇ ebon ˇ ; 4 Institute of Physical Biology, University of South Bohemia, C ˇ eske ´ Bude ˇjovice, Czech ... thylakoid membrane [7–9]. All redox cofactors are bound to a central part of the complex formed by the reaction center D1 and D2 protei...

Ngày tải lên: 16/03/2014, 18:20

... (2000) Structure of GATE- 16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p. J Biol Chem 275, 25445–25450. 7 Ichimura Y, Kirisako T, Takao T, Satomi Y, Shimonishi ... mediated by the calreticulin P-domain. On the basis of these observations, we present a detailed molecular model of the native complex. Beyond the specifics of this particular...

Ngày tải lên: 30/03/2014, 02:20

... a result of the synthesis of an excess amount of TNSALP (Fig. 1C, lanes 4–6, see the top of gel). In the present experiment, TNSALPs were expressed Molecular basis of perinatal form of hypophosphatasia ... or presence of doxycycline (DOX) and analyzed by SDS-PAGE in the absence (Nonred) or presence (Red) of 2-mercaptoethanol, followed by immunoblotting. An arrowhead indi...

Ngày tải lên: 07/03/2014, 05:20

... in active site formation by bordering the substrate bound therein. Active site of VioC Crystals of the substrate complex were obtained by crystallization of purified VioC in the presence of potassium ... B-factors was justified for the product complexes, owing to the atomic resolution of their datasets and a drop of R free of more than 2%. Research Collaboratory for S...

Ngày tải lên: 16/03/2014, 02:20

... bind to the N7 atom of guanine bases in such a motif [25]. Recognition of Co 2+ by RNA aptamers The data presented here reveal the importance of the N7 atom of purines in the organization of the ... for isotope enrichment to resolve the RNA spectrum. Therefore, in order to gain a glo- bal insight into metal ion–RNA interactions, it is often necessary to conduct structural...

Ngày tải lên: 16/03/2014, 06:20

... laboratories and are claimed to be 98% pure by the manufacturer. Solutions for ITC titra- tions were prepared by weighting the total amount of sugar on a microbalance prior to dissolving it into ... determination Attempts to crystallize PAL in absence of any carbohydrate ligands failed. To obtain crystals of ligand-free PAL, a co- crystal of the Mana(1–3)Man complex [21]...

Ngày tải lên: 23/03/2014, 11:20

... crystallographic studies of the mutant enzyme in complex with sub- strate and feedback inhibitor to reveal the structural basis for these phenomena. Crystal structure of the N64D–dTTP complex The Dm-dNK–dTTP complex ... presence of one of its substrates, thymidine, and the feedback inhibitor, dTTP. The introduction of the charged Asp residue appears to destabilize the...

Ngày tải lên: 30/03/2014, 20:20

... noninhibitory conforma- tion. Glyphosate sensitivity is partly restored by muta- tion of Ala100 to glycine, allowing glyphosate to bind in its extended, inhibitory conformation. Detoxification of glyphosate Detoxification ... to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phos- phate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in o...

Ngày tải lên: 14/02/2014, 14:20