Báo cáo khoa học: Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase docx
Báo cáo khoa học: Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase docx
... Structural basis of charge transfer complex formation by riboflavin bound to 6,7-dimethyl-8-ribityllumazine synthase Michael Koch1, Constanze Breithaupt1, ... enzyme complex. This relates to the high er dissociation constant in the riboflavin mutant complex compared t o the riboflavin wild-type complex. Ó FEBS 2004 Structural basis of charge transfer formation ... the Cc-atom of H94 and the N 7-atom of CEOL: wild-type enzyme:6.5 A˚;W27Ymutant:6.1A˚).The smallest distance between t he Cc-atom of H94 andthe inhibitor plane (N5-atom of riboflavin) ...
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Báo cáo khoa học: Structural basis of p63a SAM domain mutants involved in AEC syndrome ppt
... of p63a isfound to undergo a number of mutations. A number of point mutations have been identified in exons 13 and 14 of the p63 gene. These mutations can be classified intogroups according to ... fluorescence of W598 as the probe of measurement. The measure of stability of these pro-teins was determined by the amount of the denaturantrequired to get 50% of the protein in a denatured state([D]50%). ... upon the addition of guanidiniumhydrochloride. The tryptophan residue W598 of p63a SAMdomain (and tryptophan W561 in the case of mutantC561W) was used as a probe to monitor folding. Excitationwas...
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Báo cáo khoa học: Structural characterization of photosystem II complex from red alga Porphyridium cruentum retaining extrinsic subunits of the oxygen-evolving complex docx
... Cˇeske´Budeˇjovice;3Laboratory of Photosynthesis, Institute of Microbiology, Academy of Sciences of the Czech Republic, Trˇebonˇ;4Institute of Physical Biology, University of South Bohemia, Cˇeske´Budeˇjovice,Czech ... thylakoid membrane [7–9]. Allredox cofactors are bound to a central part of the complex formed by the reaction center D1 and D2 proteinsassociated with heterodimeric cytochrome b559(cyt b559)and ... projections revealed the PSII complex to havemaximal dimensions of 22 · 15 nm. The analysis of theside-view projections shows a maximal thickness of the PSII complex of about 9 nm including the...
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Báo cáo khoa học: Structural framework of the GABARAP–calreticulin interface – implications for substrate binding to endoplasmic reticulum chaperones potx
... (2000) Structure of GATE-16, membrane transport modulator and mammalianortholog of autophagocytosis factor Aut7p. J Biol Chem275, 25445–25450.7 Ichimura Y, Kirisako T, Takao T, Satomi Y,Shimonishi ... mediated by the calreticulin P-domain. Onthe basis of these observations, we present a detailedmolecular model of the native complex. Beyond thespecifics of this particular interaction, our model offersconceptual ... from 11.5 lm into the millimolar range. Weconclude that the tryptophan side chain plays a keyrole in the affinity of the CRT(178–188)–GABARAP complex. Characterization of complexes by NMRspectroscopyHigh-resolution...
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Báo cáo khoa học: Molecular basis of perinatal hypophosphatasia with tissue-nonspecific alkaline phosphatase bearing a conservative replacement of valine by alanine at position 406 Structural importance of the crown domain potx
... a result of the synthesis of an excess amount of TNSALP (Fig. 1C, lanes 4–6, see the top of gel). Inthe present experiment, TNSALPs were expressedMolecular basis of perinatal form of hypophosphatasia ... or presence of doxycycline (DOX) and analyzed by SDS-PAGE in the absence (Nonred) or presence (Red) of 2-mercaptoethanol,followed by immunoblotting. An arrowhead indicates the top of the gel.Molecular ... intracellulartransport of the mutant protein was similar to that of the wild-type protein, as assessed by the acquisition of Endo H resistance. Besides, the mutant protein wasfound to be incorporated into the...
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Báo cáo khoa học: Structural basis for the erythro-stereospecificity of the L-arginine oxygenase VioC in viomycin biosynthesis docx
... inactive site formation by bordering the substrate bound therein.Active site of VioCCrystals of the substrate complex were obtained by crystallization of purified VioC in the presence of potassium ... B-factors was justified for theproduct complexes, owing to the atomic resolution of theirdatasets and a drop of Rfree of more than 2%.Research Collaboratory for Structural Bioinformatics protein ... suitably positioned to be transferred onto the ferrylintermediate.ConclusionsThe assigned stereospecificity of the Cb-hydroxylationreaction of l-arginine by VioC is now proven by high-resolution...
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Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot
... bind to the N7 atom of guanine bases in such a motif [25].Recognition of Co2+ by RNA aptamersThe data presented here reveal the importance of theN7 atom of purines in the organization of the ... for isotope enrichment to resolvethe RNA spectrum. Therefore, in order to gain a glo-bal insight into metal ion–RNA interactions, it is oftennecessary to conduct structural studies of metal ... guanine bases are crucial for the creation of Co2+binding sites and that they appear to be involved in the coordination of the ion to the exposed N7 atom of the tandem guanines. Additionally,most...
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Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt
... laboratories and are claimed to be 98% pure by the manufacturer. Solutions for ITC titra-tions were prepared by weighting the total amount of sugaron a microbalance prior to dissolving it into ... determinationAttempts to crystallize PAL in absence of any carbohydrateligands failed. To obtain crystals of ligand-free PAL, a co-crystal of the Mana(1–3)Man complex [21] was transferred to artificial ... 4, this linkage conformationobserved in the con A complex is very close to thoseobserved in all PAL complexes, except for the NA2F complex and corresponds to a low energy conformation.Fig. 8....
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Báo cáo khoa học: Structural basis for the changed substrate specificity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx
... crystallographicstudies of the mutant enzyme in complex with sub-strate and feedback inhibitor to reveal the structural basis for these phenomena.Crystal structure of the N64D–dTTP complex The Dm-dNK–dTTP complex ... presence of one of its substrates, thymidine, and the feedback inhibitor,dTTP. The introduction of the charged Asp residue appears to destabilizethe LID region (residues 167–176) of the enzyme by ... phosphates are bound by the LID argi-nines and the 3¢-OH is bound to Glu172. In thesecases, Glu171 in the LID region forms a hydrogenbond to Asn64. By substitution of Asn64 to Asp in the mutantenzyme,...
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Tài liệu Báo cáo khoa học: Molecular basis of glyphosate resistance – different approaches through protein engineering doc
... noninhibitory conforma-tion. Glyphosate sensitivity is partly restored by muta-tion of Ala100 to glycine, allowing glyphosate to bindin its extended, inhibitory conformation.Detoxification of glyphosateDetoxification ... to Ala and Ala183 to Thr in 5-enolpyruvylshikimate-3-phos-phate synthase gene of E. coli (k12) and transformation of rapeseed (Brassica napus L.) in order to make toler-ance to glyphosate. Plant ... produce this cytotoxic inhibitor of glutamine synthetase have acetyltransferases thatderivatize the molecule to a noninhibitory acetylatedform (Fig. 5) [53]. The paradigm set by Nature withphosphinothricin...
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Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpNghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhNghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tổ chức và hoạt động của Phòng Tư pháp từ thực tiễn tỉnh Phú Thọ (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ