Báo cáo khoa học: Protein transport into canine pancreatic microsomes A quantitative approach potx

... calculate that 12 · 10 15 molecules of cotransla- tionally active protein translocases are present per canine pancreas, or about twice that number for a typical human pancreas. An average human ... complexes that are present in cotranslationally active protein translocases, by quantitative analysis of protein transport into pancreatic microsomes in single turnover transloc...

Ngày tải lên: 30/03/2014, 15:20

... post-translational SRP pathway has been identified in a system that tar- gets proteins into the thylakoid membrane but has no RNA requirement [42]. The post-translational cpSRP transport pathway has a ... translocation. By contrast, Sec substrate proteins are transported in an unfolded conformation in a process that requires ATP. Sec transport minimally involves SecA (an ATPase) an...

Ngày tải lên: 07/03/2014, 03:20

... chloroplast envelope. Abbreviations GAP, GTPase activating protein; GEF, guanine nucleotide exchange factor; Hsp, heat shock protein; POTRA, polypeptide -transport- associated; ppi, plastid protein ... of Toc34 (atToc33 and atToc34) [15,16], and four paralogs each of Toc159 (atToc159, atToc132, atToc120 and atToc90) [17–20] and Toc75 (atToc75-III, atToc75-IV, atToc75-I and atToc75V ⁄ atO...

Ngày tải lên: 07/03/2014, 03:20

... T, Tabata S, Seki M, Kobayashi M, Shinozaki K & Moriyasu Y (2004) Disposal of chlorop- lasts with abnormal function into the vacuole in Arabid- opsis thaliana cotyledon cells. Protoplasma 223, ... stromal domain: (a) the last approxi- mately 60 amino acids are weakly similar to a conserved motif of the mammalian co-chaperones Hsp70-interacting protein (Hip) and Hsp70/Hsp90- organizi...

Ngày tải lên: 07/03/2014, 03:20

... Medicago truncatula and Populus alba without a cleavable N-terminal targeting signal [30]. Mature proteins Analysis of the role of the mature protein in dual target- ing in several studies reveals ... widespread and conserved dual targeting is in plant evolution. An examination of proteins that are dual targeted reveals that they differ substantially in functional cate- gorization compare...

Ngày tải lên: 07/03/2014, 03:20

... unclear. Archaea probably have both co- and post-transla- tional translocation. Although co-translational trans- location is likely to be similar to that in eukaryotes and eubacteria, it is not ... structural data, with the ‘holy grail’ being a picture of an active translocon, where a channel associated with both a partner and a translocating polypeptide chain is visualized at the ato...

Ngày tải lên: 07/03/2014, 06:20

... the intermembrane space of chloroplasts by a novel pathway. J Biol Chem 274, 25181–25186. 17 Awai K, Marechal E, Block MA, Brun D, Masuda T, Shimada H, Takamiya K, Ohta H & Joyard J (2001) Two ... MGD synthase 1 mutant. Proc Natl Acad Sci USA 97, 8175–8179. 19 Miege C, Marechal E, Shimojima M, Awai K, Block MA, Ohta H, Takamiya K, Douce R & Joyard J (1999) Biochemical and topologica...

Ngày tải lên: 23/03/2014, 07:20

... Support Grant P30 CA21745, and a grant from the American Lebanese Syrian Associated Charities (ALSAC) to J. Schuetz. A major part of this work was presented at the FEBS special meeting on ABC proteins ... of gestational age and cellular differentiation. Am J Pathol 166, 39–48. 9 Van Aubel RA, Smeets PH, van den Heuvel JJ & Rus- sel FG (2005) Human organic anion transporter MRP4 (ABCC4...

Ngày tải lên: 07/03/2014, 11:20

... the paracellular route. They may also act as a physical barrier along the cell surface allowing the asymmetrical distribution of proteins and lipids between apical and basolateral domains, a phenome- non ... complex as a scaffold for the Rho family GTPases RhoA, Rac and Cdc42, and for mitogen-activated protein kinase (MAPK) and Ser ⁄ Thr kinases. These proteins regulate endothelial ce...

Ngày tải lên: 15/03/2014, 10:20

... N1 and a semantic role of a head word. All definition sentences in RSK were analyzed by JUMAN, a Japanese morphological analyzer, and KNP, a Japanese syntactic and case ana- lyzer (Kurohashi ... Semantic Analysis of Japanese Noun Phrases : A New Approach to Dictionary-Based Understanding Sadao Kurohashi and Yasuyuki Sakai Graduate School of Informatics, Kyoto University Y...

Ngày tải lên: 08/03/2014, 06:20