Báo cáo khoa học: Protein transport into canine pancreatic microsomes A quantitative approach potx

... calculate that 12 · 1015molecules of cotransla-tionally active protein translocases are present per canine pancreas, or about twice that number for a typical humanpancreas. An average human ... complexes that are present in cotranslationallyactive protein translocases, by quantitative analysis of protein transport into pancreatic microsomes in singleturnover translocation experiments, and ... Protein transport into canine pancreatic microsomes A quantitative approach Silvia Guth*, Christian Vo¨ lzing*, Anika Mu¨ ller, Martin Jung and Richard ZimmermannMedizinische...

Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx

... post-translationalSRP pathway has been identiﬁed in a system that tar-gets proteins into the thylakoid membrane but has noRNA requirement [42].The post-translational cpSRP transport pathway has a ... translocation. By contrast, Sec substrateproteins are transported in an unfolded conformation in a processthat requires ATP. Sec transport minimally involves SecA (anATPase) and the membrane-bound ... nostromal factors or ATP and, instead, is energized bythe trans-thylakoidal proton gradient [3,21,22]. Inaddition, protein substrates can be transported in a Protein transport across thylakoid...

Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf

... chloroplast envelope.AbbreviationsGAP, GTPase activating protein; GEF, guanine nucleotide exchange factor; Hsp, heat shock protein; POTRA, polypeptide -transport- associated; ppi, plastid protein ... of Toc34 (atToc33 andatToc34) [15,16], and four paralogs each of Toc159(atToc159, atToc132, atToc120 and atToc90) [17–20]and Toc75 (atToc75-III, atToc75-IV, atToc75-I andatToc75V ⁄ atOep80) ... These are thekinase(s) ⁄ phosphatase(s) that phosphorylate ⁄ dephosphorylate atToc159 and ⁄ or atToc33, as well as factors that control the GTPase hydroly-sis cycle by activation (GAPs) or facilitation...

Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

... T, Tabata S, Seki M, Kobayashi M,Shinozaki K & Moriyasu Y (2004) Disposal of chlorop-lasts with abnormal function into the vacuole in Arabid-opsis thaliana cotyledon cells. Protoplasma 223, ... stromal domain: (a) the last approxi-mately 60 amino acids are weakly similar to a conserved motif of the mammalian co-chaperonesHsp70-interacting protein (Hip) and Hsp70/Hsp90-organizing protein ... different plastid types frommultiple plant species. Plant Mol Biol 51, 175–181.58 Tanaka R, Hirashima M, Satoh S & Tanaka A (2003)The Arabidopsis-accelerated cell death gene ACD1 isinvolved...

Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt

... Medicago truncatula andPopulus alba without a cleavable N-terminal targetingsignal [30].Mature proteinsAnalysis of the role of the mature protein in dual target-ing in several studies reveals ... widespread and conserveddual targeting is in plant evolution.An examination of proteins that are dual targetedreveals that they differ substantially in functional cate-gorization compared to a ... & Whelan J (2008) Approaches to deﬁningdual targeted protein in Arabidopsis. Plant J, doi:10.1111/j.1365-313X.2008.03745.x.27 Ono Y, Sakai A, Takechi K, Takio S, Takusagawa M& Takano H...

Báo cáo khoa học: Protein transport across the endoplasmic reticulum membrane pdf

... unclear.Archaea probably have both co- and post-transla-tional translocation. Although co-translational trans-location is likely to be similar to that in eukaryotesand eubacteria, it is not ... structural data, with the ‘holy grail’ being a picture of an active translocon, where a channelassociated with both a partner and a translocatingpolypeptide chain is visualized at the atomic ... translo-cation occurs by different mechanisms in eukaryotesand bacteria. In yeast (and probably in all eukaryotes),translocation occurs by a ratcheting mechanism andinvolves, as channel partners,...

Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx

... the intermembrane space of chloroplastsby a novel pathway. J Biol Chem 274, 25181–25186.17 Awai K, Marechal E, Block MA, Brun D, Masuda T,Shimada H, Takamiya K, Ohta H & Joyard J (2001)Two ... MGDsynthase 1 mutant.Proc Natl Acad Sci USA 97,8175–8179.19 Miege C, Marechal E, Shimojima M, Awai K, BlockMA, Ohta H, Takamiya K, Douce R & Joyard J(1999) Biochemical and topological properties ... Akita M, Davila-Aponte J & Keegstra K(1997) Stable association of chloroplastic precursorswith protein translocation complexes that contain pro-teins from both envelope membranes and a...

Báo cáo khoa học: cGMP transport by vesicles from human and mouse erythrocytes potx

... Support Grant P30 CA21745, and a grant from the American Lebanese Syrian AssociatedCharities (ALSAC) to J. Schuetz. A major part of thiswork was presented at the FEBS special meeting onABC proteins ... ofgestational age and cellular differentiation. Am J Pathol166, 39–48.9 Van Aubel RA, Smeets PH, van den Heuvel JJ & Rus-sel FG (2005) Human organic anion transporter MRP4(ABCC4) is an efﬂux ... Abcc4, and an estimated apparentKm>10mm for cGMP transport by Abcg2. Thisshows that both murine transporters have a muchlower afﬁnity for cGMP than human ABCC4.ABFig. 2. Levels of ABCCs...

Báo cáo khoa học: Recent insights into cerebral cavernous malformations: a complex jigsaw puzzle under construction doc

... the paracellular route. They may also act as a physical barrier along the cell surface allowing theasymmetrical distribution of proteins and lipidsbetween apical and basolateral domains, a phenome-non ... complex as a scaffoldfor the Rho family GTPases RhoA, Rac and Cdc42,and for mitogen-activated protein kinase (MAPK) andSer ⁄ Thr kinases. These proteins regulate endothelialcell shape and polarity. ... ofglass 1Heart of glass 1 (HEG1) is a transmembrane protein ofunknown function bearing a large extracellular domainwith two epidermal growth factor-like domains, a transmembrane segment and a...

Báo cáo khoa học: "Semantic Analysis of Japanese Noun Phrases: A New Approach to Dictionary-Based Understanding" doc

... N1 and a semantic role of a head word. All definition sentences in RSK were analyzed by JUMAN, a Japanese morphological analyzer, and KNP, a Japanese syntactic and case ana- lyzer (Kurohashi ... Semantic Analysis of Japanese Noun Phrases : A New Approach to Dictionary-Based Understanding Sadao Kurohashi and Yasuyuki Sakai Graduate School of Informatics, Kyoto University Yoshida-honmachi, ... 'teacher' Time aki 'autumn' no hatake 'field' 3ji-ni 'at 3 o'clock' yomu 'read' Place Kyoto no raise 'store' heya-de 'in room'...

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