... following exposure to calcium iono- phore, indicating that they were correctly targeted into secretory granules even in the absence of serglycin. In the absence of serglycin, the fates of the serglycin-dependent ... on serglycin. Together, these findings indicate that the reduced amounts of neutral proteases in the absence of serglycin is not caused by missorting in...
Ngày tải lên: 07/03/2014, 11:20
... increase in the amount of ROS [21]; (b) loss of cytochrome c impairs the flow of electrons in the respiratory chain inducing over- reduction of the complexes, favouring the generation of ROS [16,17,22]; ... con- tribute to the latent [Ca 2+ ] c rise in the context of glutamate-induced delayed calcium deregulation; (b) the likelihood of the involvement of...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: A di-leucine sorting signal in ZIP1 (SLC39A1) mediates endocytosis of the protein doc
... detec- ted in the perinuclear region of the CHO cells with a punctate distribution in the cytoplasm of the cell (Fig. 2A). The perinuclear staining of the wt ZIP1-Myc protein was strikingly coincident ... contrast, in the presence of chloroquine, the total ZIP1-Myc accumula- tion in the CHO cells expressing the wt ZIP1-Myc pro- tein was increased to the...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
... and production of the target protein [43,44]. The results presented in this work agree with previous reports showing that the inclusion of a syn- thetic intron and the use of the bovine growth hor- mone ... 0.226). The difference between the increases in GALNS enzyme activities and mRNA levels could be explained by the presence of additional sequences within the...
Ngày tải lên: 29/03/2014, 21:20
Báo cáo khoa học: Presence of membrane ecdysone receptor in the anterior silk gland of the silkworm Bombyx mori pdf
... Presence of membrane ecdysone receptor in the anterior silk gland of the silkworm Bombyx mori Mohamed Elmogy, Masafumi Iwami and Sho Sakurai Division of Life Sciences, Graduate School of Natural ... determined using 25 n M [ 3 H]PonA and increasing amounts of proteins in individual incubations. The percentage of specific b inding increased in a prote...
Ngày tải lên: 30/03/2014, 15:20
Tài liệu Báo cáo khoa học: Specific membrane binding of the carboxypeptidase Y inhibitor IC, a phosphatidylethanolamine-binding protein family member doc
... little information is available regard- ing the phospholipid and membrane binding of the other members of the PEBP family. Consequently, the binding characteristics of PEBP proteins, including those ... the CPY-binding sites participates in regulation of the specific binding of I C to the anionic phospholipid membranes (Fig. 4A). The participation of the N-ter...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Structure and membrane interaction of the internal fusion peptide of avian sarcoma leukosis virus pdf
... that the helical structure was enhanced in association with the model membranes, p articularly in the N -terminal portion of the peptide. According to the infrared study, the peptide inserted into ... bilayer, including in particular the structural in uence of proline. Consistent with other c lass I viral fus ion proteins, the IFP o f ASLV inserts into the membra...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Solution and membrane-bound chaperone activity of the diphtheria toxin translocation domain towards the catalytic domain" doc
... equilib- rium of the C domain in favor of the MG state. The T domain favors the interaction of the C domain with the membrane, because it brings the C domain in its MG state into the vicinity of the bilayer, ... transloca- tion, the C domain and only the 63 N-terminal amino acids of the T domain are present on the trans side of the membrane [24,2...
Ngày tải lên: 14/03/2014, 22:20
Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
... submitted), suggesting the presence of other Src-binding sites in RPTPa. Taken together, phosphorylation of Tyr789 is not the only determinant in Src binding. Instead, we provide evidence that other features of ... domains of RPTPs are involved in protein–protein interactions [16–18,20,28]. We investigated whether the introduc- tion of inactivating mutations in RPTPa...
Ngày tải lên: 15/03/2014, 10:20
Báo cáo khoa học: Presence of melanocortin (MC4) receptor in spiny dogfish suggests an ancient vertebrate origin of central melanocortin system pot
... been found in brain tissue [8,41]. The DreMC4 receptor was also found in the brain but rather surprisingly also in the eye, gastro- intestinal tract and ovaries. In chicken, the MC4 receptor is expressed ... RT-PCR. The results of the RT-PCR are shown in Fig. 5. The SacMC4 receptor was expressed in five brain regions (Fig. 6) but not in any of the periph...
Ngày tải lên: 23/03/2014, 20:22