Báo cáo khoa học: Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase docx

... Mode of action of the microbial metabolite GE23077, a novel potent and selective inhibitor of bacterial RNA polymerase Edoardo Sarubbià, Federica Monti*, Emiliana Corti, Anna Miele and Enrico ... from rifampicin, the data shown in the previous paragraphs indicate that the two compounds share a number of common features. Both are potent and...

Ngày tải lên: 30/03/2014, 15:20

... Reverse Transcription System (Thermoscrip RT, Invitrogen). Human GHR was amplified from cDNA with primers ACACTCAAGAATGGACTCAAG and TGTAAATTGG CTCATCTGAG under the following conditions: denatura- tion at ... cycles. Cloning of p21 WAF/CIPI and transfections Human p21 WAF ⁄ CIPI cDNA derived from the C children was amplified using the following primers: GGAAAT CATGTCAGAACCGGC and C...

Ngày tải lên: 23/03/2014, 10:21

... basis of the available structural data of canonical mammalian class IIIa and mycobacterial class IIIc catalytic domains [5,7,9,25] nor do they parallel the findings on the noncanonical class IIIc AC ... purified and stable in the absence of protease inhibitors. Actually, none of the previously reported mutants of the canonical lysine-aspartate couple in mammalian and...

Ngày tải lên: 07/03/2014, 21:20

... +RNA0 – +RNA1 +RNA2 +RNA3 +RNA4 +RNA5 C – RNA1 RNA2 RNA3 RNA4 RNA5 CGGCC +RNA1 CGGC +RNA2 CGG +RNA3 C +RNA4 +RNA5 ACCTCGTATAC RNA0 ACCTCGTATA RNA1 ACCTC RNA2 ACC RNA3 AC RNA4 RNA5 Fig. 4. Map ping of ... rotein and the +RNA0 (Fig. 2B). In t he same way, the E MSA containing a fixed a mount of the NS5B and the radiolabeled RNA0 in the presence of incr...

Ngày tải lên: 19/02/2014, 16:20

... NA TGCARRAAYATHTTYTCCAG Deg RPE65-Rev AYRAAYTCRWRBCCYTTCCA RPE6 5a- Fwd NM_200751 GCGGCCGCCACCATGGTCAGCCGTTTTGAACAC RPE6 5a- Rev GATATCTTATGGTTTGTACATCCCATGGAAAG RPE65c-Fwd NM_001113653 GCGGCCGCCACCATGGTCAGCCGTCTTGAACAC RPE65c-Rev ... GCGGCCGCCACCATGCATCATCACCATCAC CATGTCAGCCGTTTTGAACAC RPE65c-His-Fwd NM_001113653 GCGGCCGCCACCATGCATCATCACCATCAC CATGTCAGCCGTCTTGAACAC Y. Takahashi et al. A n...

Ngày tải lên: 14/02/2014, 14:20

... b-1,6-GlcNAc branched tri-antennary and tetra-antennary oligosac- charides in a 5 b 1 [56]. Similarly, characterization of the carbohydrate moieties in a 3 b 1 from nonmetastatic and metastatic human melanoma cell ... M, Yamaguchi N, Kangawa K & Taniguchi N (1993) Purification and characterization of UDP-N-acetylglucosamine: alpha-6- D-mannoside beta 1-6N-acetylglucosaminyl...

Ngày tải lên: 18/02/2014, 17:20

... vivo evolution of a tRNA synthetase–tRNA pair from Methanococcus jannaschii capable of accepting and loading glycosylated amino acids has allowed the introduction of O-b-d-GlcNAc-l-Ser [25] and O -a- d-GalNAc-l-Thr ... withstanding these clear demonstrations of the utility of linear ligation assembly, a convergent chemo- selective approach can offer the key advantage...

Ngày tải lên: 18/02/2014, 17:20

... by the electrical potential across the membrane) and occasionally bind to and thus compensate the negative charge on the rotor. The freed positive charge on the stator attracts the next negative ... has only 10 membrane-buried negative charges that are essential for binding the ion and also for the rotational mechanism of the ring. The c ring of I. tartaricus h...

Ngày tải lên: 18/02/2014, 17:20

... (R-Smads, Smad1, -2, -3, -5 and -8), which, upon phosphorylation, are activated and bind to a common mediator Smad (Co-Smad, Smad4). The complexes move into the nucleus and act as regulators of ... zebra- fish was also shown to possess Chordin cleavage activity [26]. The transcripts are detected throughout the early gastrula stage embryo but toward the end of gastrulation...

Ngày tải lên: 19/02/2014, 00:20

... mutant of the variant at both pH 7 and pH 5. Each panel is a plot of heat change on ligand addition (kcalÆmole )1 ) against the ligand ⁄ stefin molar ratio for one of the proteins analysed. The ... applied on a Formvar and carbon coated grid. After 5 min the sample was soaked away and stained with 1% (v ⁄ v) uranyl acetate. Samples were observed with a Philips (Amst...

Ngày tải lên: 19/02/2014, 06:20