Báo cáo khoa học: The crystal structure of the tryptophan synthase b2 subunit from the hyperthermophile Pyrococcus furiosus Investigation of stabilization factors pot

... 2004 Crystal structure of tryptophan synthase b 2 subunit alone (Eur. J. Biochem. 271) 2635 The crystal structure of the tryptophan synthase b 2 subunit from the hyperthermophile Pyrococcus furiosus Investigation ... examined by DSC. This is the first report of the X-ray structure of the tryptophan synthase b 2 subunit alone, although...

Ngày tải lên: 30/03/2014, 14:20

... plateau of recombinant heterodimer forma- tion was reached  48 h after the onset of the experi- ment. The yield of the rPAB association process was 0.2 mg, corresponding to 20% of the total theoretically obtainable ... spectroscopy. The cloning process will enable the production of soluble and active homogeneous protein, which is desirable to the study of its use...

Ngày tải lên: 07/03/2014, 16:20

... con- sists of the following steps: (1) Given a web site, the root page and web pages directly linked from the root page are downloaded. Then for each of the downloaded web page, all of its ... l .N D i refers to the HTML tag of the node D i N ; j C.N D i refers to the j th child of the node D i N ; [ ] nm C , D i .N refers to the consecutive sequence...

Ngày tải lên: 08/03/2014, 02:21

... sequence of words. The distance of two surface patterns is defined as the fraction of the LD value to the length of the longer sequence. For estimating the number of clusters k, we ap- ply the stability-based ... characterize the semantic relation between the pair by leveraging the vast size of the Web. Our hypothesis is that there exist some key terms and pat...

Ngày tải lên: 23/03/2014, 16:21

... However, these structures do not show the coordinates of the atoms in Fig. 6. Superposition of the representative NMR open conformer of the C-domain (red and blue) on the crystal structure [20] of the complex ... ª 2010 FEBS 2613 the a-helices on one side of the b -sheet and two on the other side. The rmsd of the heavy atoms (Ca, C, and N) of the prot...

Ngày tải lên: 06/03/2014, 11:20

... establish the preferred geometry of the precursor. We now report the chemical synthesis, bio- chemical production, and solution structure of preCbnB2, and compare it with the structure of the mature bacteriocin, ... Solution structure of preCbnB2 in 70% trifluoroethanol. The positions of the N-terminus and C-terminus are indicated, as are the residues at the s...

Ngày tải lên: 07/03/2014, 15:20

... to the indole part of Fig. 2. pH dependence of the oligomeric state of EcIPDC. Volume fractions were calculated from the scattering patterns with the program OLIGOMER in the absence of cofactors ... indolepyruvate (Fig. 1). In the course of the establishment of the equilibrium % 85% of the enol form is converted into the ketone (half-time % 8 min at 20 °C)...

Ngày tải lên: 08/03/2014, 02:20

... Stereo views of the solution and crystal structures of DnaG-C. (A) Superposi- tion of the backbone atoms of residues 447–581 of the 20 NMR conformers of DnaG-C representing the NMR structure (Table ... and boundaries of helix 6 (Fig. 3D,E), showing that this helix can be separated from the core of the structure. The solution structure of DnaG-C identifi...

Ngày tải lên: 16/03/2014, 12:20

... This kind of protein is exemplified by the Thermus thermophilus protein, PDB entry 2B3B [28]. The mode of binding the monosaccharide is completely different in terms of orientation of the sugar, ... similarity between the Fig. 3. Extension of the GGal site. Stereo- view of the residues lining the water-filled tunnel that extends from the glyceryl moiety to the...

Ngày tải lên: 23/03/2014, 04:21

... evolution of the primary structure of the N-terminal regions of TTR and the biological function of TTR. Results Synthesis of recombinant human TTR In the crocodile, TTR is only synthesized in the liver during ... T4). In the other, the N-terminal region of crocTTR was deleted. The affinity of T3 and T4 to both TTRs was studied and the results are a powerful de...

Ngày tải lên: 23/03/2014, 10:21