Báo cáo khoa học: Tyrosine phosphorylation of calponins Inhibition of the interaction with F-actin pot

... increased their phosphotyrosine content. The lack of F-actin protection against tyrosine phosphorylation of calponins contrasts with the F-actin- induced blocking effect on the enzymatic Ser/Thr phosphorylation ... functional effects of its phosphorylation relative to those found with the two other calponins. Next, we examined the influence of F-actin on...

Ngày tải lên: 30/03/2014, 14:20

... threonine phosphorylation of tau [20,21]. To determine whether the tyrosine phosphorylation status of tau also affects the binding of tau to Fyn-SH3, CHO cells were cotransfected with Fyn together with ... increased tyrosine phosphory- lation in cells. Tyr18 of tau plays an important role in the interaction of tau with Fyn-SH2 To determine whether the tyrosi...

Ngày tải lên: 14/02/2014, 14:20

... eluted with 55 mL of the same buffer, first alone and then with a 200 mL of a decreasing gradient of ammonium sul- phate in 20 mm Tris⁄ HCl buffer (pH 8.0) with a flow of 0.8 mLÆmin )1 . Fractions of ... 97% inhibition of BHLmL2 and BHAb, respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The pH optima...

Ngày tải lên: 18/02/2014, 14:20

... in the interaction with the BACE-1 cytoplasmic domain [74]. The cellular form of the prion protein also negatively regulates b-secretase cleavage of APP, probably through its raft interaction with ... small peptide substrates as they lack many of the subsite and other interactions of the genuine protein substrate. Neverthe- less, such studies have led to the deve...

Ngày tải lên: 07/03/2014, 00:20

... [20,21]. The ability of farnesol administration to alter the prenylation of the small G proteins Ras and Rap1A has been previously tested and neither the prenylation event nor the ability of the G ... role of phospholipids in the PLD signaling pathway We next determined the ability of PC (upstream of the alcohol block) and DAG (downstream of the alcohol block...

Ngày tải lên: 23/03/2014, 15:21

... terms to the phonetic transcription of the second output in the n-best list, as compared to the other outputs. One obvious problem with this method is that it ignores the relative ordering of the n-best ... are caused by a lack of evidence for the map- ping of the grapheme representing the sound /k/ in the transliteration training data with the phoneme /Ù/. I...

Ngày tải lên: 17/03/2014, 00:20

... step 6. If the user subsequently goes past step 17 of the subprocedure, the system warns that the user has gone past the required steps, and suggests that they close the procedure. Other important ... it jumps directly to the next section. Step 51 points the user to a subprocedure. The spoken version asks if the user wants to execute the steps of the subproce- dure...

Ngày tải lên: 31/03/2014, 03:20

... translations of source lexical items has been one of the hardest problems in MT. Because languages have their own ways of reflecting the structure of the world in their lexi- cons, and the process of ... [WEAR:MI-NI-TSUKERU] and if the argument-2 of the event belongs to the sort [SHOES:KUTSU], then the event also belongs to [WEAR:HAKU]. All the event-so...

Ngày tải lên: 01/04/2014, 00:20

... basis for the effect of SPSP motif phosphorylation on the interaction of SF1 with U2AF 65 . A comprehensive set of data supports the functional importance of the interaction of SF1 with U2AF 65 .In mammals, ... suggesting that phosphorylation of these sites might regulate the formation of the SF1-U2AF-RNA complex. We first tested the effect of phospho...

Ngày tải lên: 19/02/2014, 07:20

... affinity of the binding interactions was evaluated by measuring the changes of fluorescence polarization of the peptides at each step of titration with the Grb4 SH2 domain, i.e. the binding isotherms ... details of these signaling events have yet to be investigated, many of the molecular interactions down- stream of the Eph and ephrin molecules appear to lead to t...

Ngày tải lên: 19/02/2014, 12:20