Báo cáo khoa học: Tet repressor residues indirectly recognizing anhydrotetracycline pptx

... Tet repressor residues indirectly recognizing anhydrotetracycline Peter Schubert*, Klaus Pfleiderer and Wolfgang Hillen Lehrstuhl ... sequence variants of the tetracycline repressor (TetR) to investigate ligand recognition of two different tetracycline (tc) derivatives, tc and anhydrotetra- cycline (atc). TetR regulates resistance ... 91318528081, E-mail: whillen@biologie.uni-erl...

Ngày tải lên: 30/03/2014, 13:20

... constants a ,(·10 7 M )1 ) [4-ddma-atc-Mg] + [atc-Mg] + TetR TetR i2 TetR i2 -V99N TetR i2 -L17A TetR TetR i2 TetR i2 -V99N TetR i2 -L17A TetR M + [tc-Mg] + ?TetR M [tc-Mg] + 0.3 b 132 b 119600 b 1.7 b < 0.02 c < 0.02 c TetR D + [tc-Mg] + ? ... the crystal structures. Abbreviations Atc, anhydrotetracycline; 4-ddma-atc, 4-de-dimethylamino -anhydrotetracycline; tc, tetracycline; TetR, te...

Ngày tải lên: 20/02/2014, 01:20

... Barker, J.J.,Primrose,W.U.,Tyler,P.D.&Lewendon,A.(2003) Inhibitors of phosphopantetheine adenylyltransferase. Eur. J. Med. Chem. 38, 345–349. 172 T. Kupke (Eur. J. Biochem. 271) Ó FEBS 2003 Active-site residues and amino acid specificity ... Thr. Keywords: coenzyme A biosynthesis; 4¢-phosphopantethe- ine; 4¢-phosphopantothenoylcysteine synthetase; Dfp flavo- protein; cysteine metabolism....

Ngày tải lên: 19/02/2014, 12:20

... (underlined residues in Fig. 1) are tallied. Only the totals are given for murine sHsps. Residues that promote disorder are in bold and those that promote order are in italic [32]. Residues are ... domain with other residues, possibly through decreased electro- static interactions and increased electrostatic repulsion [30]. Similarly, it is likely that the glutamic acid residues in t...

Ngày tải lên: 07/03/2014, 04:20

... with very extended (GCC) n repeats. Mammalian ZF5 protein was first identified as a transcriptional repressor of the murine c-myc gene. Although ZF5-mediated transcriptional activation has been reported ... FMR1 gene. These data clearly support the assertion that endogenous ZF5 acts as a transcriptional repressor of the human FMR1 gene. Given that FMR1 inactivation in FMR patients has been...

Ngày tải lên: 07/03/2014, 05:20

... 7, 1884–1897. Active site residues in aloesone synthase I. Abe et al. 218 FEBS Journal 273 (2006) 208–218 ª 2005 The Authors Journal compilation ª 2005 FEBS Active site residues governing substrate ... followed by Claisen-type cyclization of the enzyme-bound tetra- ketide intermediate, leading to formation of naringenin chalcone (4,2¢,4¢,6¢-tetrahydroxychalcone) (Fig. 1A) [1,2]. The in vi...

Ngày tải lên: 16/03/2014, 14:20

... The residues that constitute the insulin receptor-binding area are shown in red, and the residues important for the direction to insulin receptor are shown in orange (D and E). In (F), four residues ... substitution, and two residues in orange are located in a similar space as the residues in orange of (E). The opened yellow squares showed a similar topology of side-chains of putati...

Ngày tải lên: 17/03/2014, 03:20

... Active site residues and mechanism of UDP-glucose dehydrogenase Xue Ge 1 , Lisa C. Penney 1 , Ivo van de Rijn 2 and ... Cys/Ser260 was positioned appropriately to participate in covalent catalysis, as expected. Other active site residues that could potentially play roles in the hydride transfer and/or hydro- lysis steps were ... the hydride transfer steps (presumably both hydride transfer...

Ngày tải lên: 23/03/2014, 12:20

... miR-17-92 downregulates the expression of E2F and Myc [52]. In tumor progression, the tran- scription repressors ZEB1 and SIP1 and the miR-200 family of miRNAs provide a double-negative-feedback loop

Ngày tải lên: 14/02/2014, 19:20

... any such piece as the list for a document, we are treating them as connected, and therefore are indirectly taking their structure into account. We have also to make our own choice of the pieces ... information in two ways: 1) directly, using the occurrences of objects or prop- erties; 2) indirectly, using the occurrences of objects or prop- erties to obtain resemblance coefficient...

Ngày tải lên: 19/02/2014, 19:20