Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot

... some of these recombinants were able to reduce the life span of infected insects. The tropical ant Paraponera clavata is a predator of small animals such as insect larvae. Its venom contains a potent insect- specific ... 2004 Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide...

Ngày tải lên: 30/03/2014, 13:20

... Boston, MA, USA). Quantitative RT-PCR Total RNA from RAW 264.7 macrophages was extracted using Rnaeasy Mini Kit (Qiagen spa, Milan, Italy) and RNase-Free DNase Set (Qiagen) according to the manufac- turer’s ... translocation (Fig. 5B) was also significantly increased by incubation of the cells with AA-treated and untreated quartz, at all particle concentrations (analysis of varianc...

Ngày tải lên: 23/03/2014, 10:20

... protein kinase 1 (DAPK-1) is a Ca 2+ ⁄ calmodulin-regulated serine ⁄ threonine kinase composed of multiple functional domains, including a kinase domain, a calmodulin-binding domain, eight ankyrin ... of DAPK-1’s ankyrin-repeat region, P-loop, and cytoskel- etal binding domain, and a unique tail of 42 amino acids encoded by intron 20–21 of the DAPK-1 gene. In our examina...

Ngày tải lên: 18/02/2014, 17:20

... acetaldehyde and acrolein induce the expression of metallothionein and modulate protein tyrosine phosphatase activity in a zinc-dependent way. Since minute changes in the availability of cellular zinc have ... groups of lysines in MT2 were carbamoylated with potassium cyanate and the modified protein was assayed for zinc release as described above. Acetaldehyde releases a...

Ngày tải lên: 19/02/2014, 06:20

... the increase in absorbance due to formation of NADH was measured at 340 nm over a linear range of 2–5 min. Mutarotase assay Mutarotase activity was measured with a DIP-360 polari- meter (Jasco). ... taken at 1-min intervals for 6 min. The rate of the nonenzymatic turnover was subtracted from the initial rate of the enzymatic reaction. Mutarotase activity was also assayed using...

Ngày tải lên: 19/02/2014, 12:20

... enzyme consisting of an RNase H domain and an acid phosphatase domain Naoto Ohtani 1 , Natsumi Saito 1 , Masaru Tomita 1 , Mitsuhiro Itaya 1,2 and Aya Itoh 1 1 Institute for Advanced Biosciences, ... 88, 12–19. 19 Ohtani N, Yanagawa H, Tomita M & Itaya M (2004) Identification of the first archaeal type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI can cleave an RN...

Ngày tải lên: 19/02/2014, 18:20

... Behaviors: A Re-ranking Based Approach Yabin Zheng Zhiyuan Liu Lixing Xie State Key Laboratory on Intelligent Technology and Systems Tsinghua National Laboratory for Information Science and Technology ... relevant candidates earlier. Finally, our method is unsu- pervised and language independent, which means that we do not require any training set or manual labeling efforts....

Ngày tải lên: 20/02/2014, 04:20

... acquire alternating verbs from large balanced corpora by using partial- parsing methods and taxonomic information, and discuss how corpus data can be used to quantify lin- guistic generalizations. ... not argu- ments but adjuncts (Jackendoff, 1990) and can appear on any verb with which they are semantically compatible. taining for-PPs were randomly selected from the parser&a...

Ngày tải lên: 20/02/2014, 19:20

... unfolding was then examined by assaying the catalytic activity after complete removal of the denaturant. As shown in Fig. 1, SsMTAPII and PfPNP are able to refold with a recovery of catalytic activity ... removal of GdnCl, was analyzed by catalytic activity measurements performed under standard condi- tions. Reactivation assay of SsMTAPII, PfPNP and their CXC-lacking mutants T...

Ngày tải lên: 07/03/2014, 00:20

... contrast, when searching in a constrained space rather than the raw space, the constrained tagger (beam=5) is 10 times fast as the baseline and the tagging accuracy is even moderately improved, increasing ... the ambiguity problem that a Chinese character may appear in any relative position in a word and the out -of- vocabulary (OOV) problem that it is impossible to observe al...

Ngày tải lên: 07/03/2014, 18:20