Báo cáo khoa học: Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide pot

... some of these recombinants were able toreduce the life span of infected insects.The tropical ant Paraponera clavata is a predator of smallanimals such as insect larvae. Its venom contains a potent insect- speciﬁc ... 2004 Poneratoxin, a neurotoxin from ant venom Structure and expression in insect cells and construction of a bio-insecticide Ewa Szolajska1, Jaroslaw Poznanski1, Miguel Lo´pez Ferber2, Joanna ... 5¢-GAATTCATGCTACTAGTAAATCAG-3¢ (number 1) and downstream primer with a sequence complementary to the 5¢ end of poneratoxin gene and 3¢ end of a signal peptide: 5¢-CAGAAGCGGAAGAAAGCATGCAAAGGCAGA-3¢...

Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages pdf

... Boston, MA, USA).Quantitative RT-PCRTotal RNA from RAW 264.7 macrophages was extractedusing Rnaeasy Mini Kit (Qiagen spa, Milan, Italy) and RNase-Free DNase Set (Qiagen) according to the manufac-turer’s ... translocation (Fig. 5B) was alsosigniﬁcantly increased by incubation of the cells with AA-treated and untreated quartz, at all particleconcentrations (analysis of variance, P < 0.001). In both ... value (analy-sis of variance, P < 0.005). COX-2 expres-sion in QA samples was signiﬁcantlyinhibited (*) by the presence of catalase and mannitol (QAc and QAm versus QA, analy-sis of variance,...

Tài liệu Báo cáo khoa học: An alternative transcript from the death-associated protein kinase 1 locus encoding a small protein selectively mediates membrane blebbing pdf

... protein kinase 1 (DAPK-1) is a Ca2+⁄ calmodulin-regulated serine ⁄ threonine kinasecomposed of multiple functional domains, including a kinase domain, a calmodulin-binding domain, eightankyrin ... of DAPK-1’s ankyrin-repeat region, P-loop, and cytoskel-etal binding domain, and a unique tail of 42 aminoacids encoded by intron 20–21 of the DAPK-1 gene. In our examination of DAPK-1 and s-DAPK-1 ... occur in human cancers.To begin functional studies of s-DAPK-1, thes-DAPK-1 cDNA was cloned into a Flag–Myc vector(Fig. 2A) , which contains an N-terminal Flag tag and a C-terminal Myc tag, and...

Tài liệu Báo cáo khoa học: Aldehydes release zinc from proteins. A pathway from oxidative stress⁄lipid peroxidation to cellular functions of zinc pptx

... acetaldehyde and acrolein induce the expression of metallothionein and modulate protein tyrosine phosphatase activity in a zinc-dependentway. Since minute changes in the availability of cellular zinc have ... groups of lysines in MT2 were carbamoylated with potassiumcyanate and the modiﬁed protein was assayed for zincrelease as described above. Acetaldehyde releasesalmost the same amount of zinc from ... CA).PAR metal transfer assayMetallochromic indicators provide a rapid means of investi-gating metal–protein equilibria [58,59]. PAR is such anindicator. Binding of zinc ions changes its absorbance...

Tài liệu Báo cáo khóa học: UDPgalactose 4-epimerase from Saccharomyces cerevisiae A bifunctional enzyme with aldose 1-epimerase activity docx

... the increase in absorbance due to formation of NADH was measured at 340 nm over a linear range of 2–5 min.Mutarotase assayMutarotase activity was measured with a DIP-360 polari-meter (Jasco). ... taken at 1-min intervals for 6 min. The rate of thenonenzymatic turnover was subtracted from the initial rate of the enzymatic reaction.Mutarotase activity was also assayed using the NAD+ and ... Sequence analysis indicates thatthe yeast epimerase has an N-terminal domain (residues1–377) that shows signiﬁcant similarity with Escherichia coli and human UDPgalactose 4-epimerase, and a C-terminaldomain...

Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... enzyme consisting of an RNase Hdomain and an acid phosphatase domainNaoto Ohtani1, Natsumi Saito1, Masaru Tomita1, Mitsuhiro Itaya1,2 and Aya Itoh11 Institute for Advanced Biosciences, ... 88,12–19.19 Ohtani N, Yanagawa H, Tomita M & Itaya M (2004)Identiﬁcation of the ﬁrst archaeal type 1 RNase H gene from Halobacterium sp. NRC-1: archaeal RNase HI cancleave an RNA-DNA junction. ... RNase H domain of RT fusedwith one APase or not. Nevertheless, the SCO2299protein examined here is a bifunctional enzyme con-sisting of an RNase H domain and an APase domain, and it is a novel...

Tài liệu Báo cáo khoa học: "Growing Related Words from Seed via User Behaviors: A Re-ranking Based Approach" pdf

... Behaviors: A Re-ranking Based Approach Yabin Zheng Zhiyuan Liu Lixing Xie State Key Laboratory on Intelligent Technology and Systems Tsinghua National Laboratory for Information Science and Technology ... relevant candidates earlier. Finally, our method is unsu-pervised and language independent, which means that we do not require any training set or manual labeling efforts. The rest of the paper ... is reasonable to measure relatedness between words by analyzing user behaviors. Second, we take the advantage of se-mantic features using search engine to reorder candidate words. We aim to...

Tài liệu Báo cáo khoa học: "Acquiring Lexical Generalizations from Corpora: A Case Study for Diathesis Alternations" pdf

... acquire alternating verbs from large balanced corpora by using partial- parsing methods and taxonomic information, and discuss how corpus data can be used to quantify lin- guistic generalizations. ... not argu- ments but adjuncts (Jackendoff, 1990) and can appear on any verb with which they are semantically compatible. taining for-PPs were randomly selected from the parser's output and ... as follows: (a) a verb partic- ipates in the dative alternation if it has the double object and 'V NP1 to NP2' frames and (b) a verb Dative Alternation Alternating V NPI NP2 allot,...

Báo cáo khoa học: Purine nucleoside phosphorylases from hyperthermophilic Archaea require a CXC motif for stability and folding pot

... unfolding was then examined by assayingthe catalytic activity after complete removal of thedenaturant.As shown in Fig. 1, SsMTAPII and PfPNP are ableto refold with a recovery of catalytic activity ... removal of GdnCl, was analyzed by catalyticactivity measurements performed under standard condi-tions.Reactivation assay of SsMTAPII, PfPNP and theirCXC-lacking mutantsThe activity of SsCSC and ... thereactivation of scrambled RNaseA (sRNaseA). Asshown in Fig. 4, after 210 min of incubation in thepresence of SsCSC and PfCGC, inactive sRNaseAshows a 5.5-fold and 3.6-fold activation, respectively,compared...

Báo cáo khoa học: "Exploring Deterministic Constraints: From a Constrained English POS Tagger to an Efﬁcient ILP Solution to Chinese Word Segmentation" ppt

... contrast,when searching in a constrained space rather thanthe raw space, the constrained tagger (beam=5) is 10times fast as the baseline and the tagging accuracyis even moderately improved, increasing ... the ambiguity problem that a Chinesecharacter may appear in any relative position in a word and the out -of- vocabulary (OOV) problem thatit is impossible to observe all words in training data,CWS ... for tagging, and runs in O(nT2) when searching in an unconstrainedspace. On the other hand, consider searching in a constrained space. Suppose that among the m un-constrained words, m1of...

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