Báo cáo khoa học: Identifying determinants of NADPH specificity in Baeyer–Villiger monooxygenases docx

... 3-amine group of the coenzyme analog and the O 4 of the isoalloxazine ring of the flavin cofactor, which would specifically promote AADP + binding. Such a ligand–cofactor interaction is in line ... sequences contain two dinucleo- tide-binding sequence motifs (Rossmann-fold motifs) – GxGxxG – which are involved in binding of the ADP moieties of FAD and NADPH [27]. This distinguis...

Ngày tải lên: 30/03/2014, 13:20

... addition of CtCBM11. In general, a progressive line broadening of all the cellohexaose protons was observed during titration with increasing amounts of protein, which can be understood as a result of ... (Fig. 1III–V), which could indicate that the corre- sponding hydroxyl groups are involved in protein binding. The results for the linebroadening measurements of protons H1a in...

Ngày tải lên: 18/02/2014, 17:20

... stabil- izing solutes reflects the strengthening of the hydrogen bond network. These findings are in line with an ear- lier study about the effect of DGP on the dynamics of Structural determinants of ... of Southampton, UK In spite of the extensive accumulation of data on pro- tein structure, the molecular determinants of protein thermal stability remain elusive. Also, t...

Ngày tải lên: 30/03/2014, 15:20

... becom- ing pupae in about a week after spinning (Fig. 1B). Isovaleric acid seems to be the cause of prepupal lethality because injection of isovaleric acid into normal spinning larva induces ... the sku mutant accumulates branched-chain amino acids in haemolymph, this mutant may be useful in the investigation of unique branched-chain amino acid catabolism in insects. Abbreviatio...

Ngày tải lên: 18/02/2014, 04:20

... chemokines stimu- lated mainly by in ammatory cytokines, including IL-1b, TNF-a and IFN-c. (B) Chemokines expressed in the joint recruit leukocytes into the joints. In addition to functioning in ... examination of affected rheu- matoid joints reveals extensive RANTES ⁄ CCL5 expression in the synovial lining and sublining layers [22]. The expression of RANTES ⁄ CCL5 in cultured...

Ngày tải lên: 18/02/2014, 18:20

... 1. cDNA cloning and sequencing cDNA was prepared from soft tissues of a single A. rubens specimen. As a starting point, we used degenerate primers designed from the N-terminal sequence determined by ... peptide of 16 amino acids and its cleavage site. Ten more amino acids are found in the predicted translated protein upstream of the serine that is found to be the N-terminal residue o...

Ngày tải lên: 19/02/2014, 12:20

... that the N-terminal domain is involved in protein–protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible. From ... the bovine complex a large subdomain is made up of  20 integral membrane proteins contributing > 60 transmem- brane segments. Some of these must be intimately involved in proton p...

Ngày tải lên: 19/02/2014, 16:20

... finding is that combining both kinds of metrics gives top performance according to ev- ery meta-evaluation criteria. In addition, our Com- bined System Test shows that, when training a combined ... Acceptable. In Proceedings of the Joint 21st International Conference on Com- putational Linguistics and the 44th Annual Meet- ing of the Association for Computational Linguistics (COLING-...

Ngày tải lên: 20/02/2014, 07:20

... clustering task, with over 15 research groups submitting results in each campaign. Since the re- lease of the first datasets, this task is becoming an increasingly popular research topic among Infor- mation ... person. In a typical hiring process, for instance, candidates are eval- uated not only according to their cv, but also ac- cording to their web profile, i.e. information about them...

Ngày tải lên: 20/02/2014, 09:20

... this protease at pH9 [10]. Further interest in the aromatic stacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic ... and W169A, deviates from the original position. In the struc- tural deviation, the solvent ASA of the side-chain of His210 increased with the decrease in size of the side-ch...

Ngày tải lên: 21/02/2014, 03:20