Báo cáo khoa học: Diversity of metallothioneins in the American oyster, Crassostrea virginica, revealed by transcriptomic and proteomic approaches potx

... metallothioneins in the American oyster, Crassostrea virginica , revealed by transcriptomic and proteomic approaches Matthew J. Jenny 1 , Amy H. Ringwood 4 , Kevin Schey 2 , Gregory W. Warr 3 and Robert ... on the number and configuration of the cysteine motifs. The Ônoncoding exonÕ region represents the b-domain region of the transcript that has been...

Ngày tải lên: 30/03/2014, 13:20

... 3). Analysis of the effect of adenine nucleotides on the binding of NBD1 to Hsc70 To further explore the effect of adenine nucleotides on the NBD1–Hsc70 interaction, we quantified the effect of ATP and ... ADP on the binding of wt-mNBD1 and F508del-mNBD1 to immobilized Hsc70. As shown by the solid lines in Fig. 5A,B, increasing concentrations of ATP caused a...

Ngày tải lên: 23/03/2014, 05:22

... A o and A t denote the intensity of protein bands of intact unhydro- lized protein at the beginning and at the fixed time of incu- bation) against the time of hydrolysis. Chaperone-like activity The chaperone-like ... well as in the binding of protein substrates to sHsp [17]. The importance of this part of molecule of the sHsp is supported by the fact...

Ngày tải lên: 23/03/2014, 07:20

... [D]-PDMP and EGF (Fig. 3D), indicating that EGF is not determinant in defining the retention of ErbB2 into the lipid rafts. The effect of [D]-PDMP treatment was partially abolished by addition of exogenous ... fractions of the plasma membrane, indicating, by a novel approach, that gan- gliosides play a key role in the retention of this protein in lipid rafts. Th...

Ngày tải lên: 23/03/2014, 10:21

... obtained by determining their tissue expression patterns, elucidating their RNA-bind- ing specificities, and identifying the genes that they control. Ultimately, understanding the function of the diverse ... 85–112 of U2AF 65 interact with U2AF 35 , and this association further strengthens the binding to the Py-tract [18]. Although it is not a member of the serine-argin...

Ngày tải lên: 23/03/2014, 10:20

... con- taining atRE (A), liposomes containing atRE in the presence of 1m M bipyridine (B) and liposomes containing atRE, in the presence of 1 m M bipyridine and 6 mM FeSO 4 (C). The generated retinoids were ... magnification images of the boxed area in (B4) showing RPE65c staining (B5), GS staining (B6), merged RPE65c and GS staining (B7), and DAPI staining (B8), resp...

Ngày tải lên: 14/02/2014, 14:20

... handedness, and two of the rings face in the opposite direction in the membrane to the other two, forming the same pattern as in the AFM surface representation of Fig. 2A. By comparison with the ... charge on the stator attracts one of the negative charges on the ring and thus keeps the ring in place [34–36]. How- ever, the system is not stiff but i...

Ngày tải lên: 18/02/2014, 17:20

... Follistatin, Chordin and Noggin [12]. These proteins are antagonists of BMP and other members of the TGF-b family, binding to and inhibit- ing these signaling molecules from binding to their receptors ... wild-type embryos, the din mutant is not rescued. Expression of chordin is induced by Sizzled in embryos with Chordin, but not in the absence of Chordin. These resu...

Ngày tải lên: 19/02/2014, 00:20

... within the intermembrane space, and the proteolysis must therefore represent a loss of 2 kDa from the N-terminus. Given there are seven arginine and lysine residues spread through the N-terminal stretch ... of the outer membrane proteins eluted from the column were resolved by SDS ⁄ PAGE. After briefly Coomassie-staining the gels, bands representing proteins of inter...

Ngày tải lên: 19/02/2014, 07:20

... game situation. In the Figure 5, we have illus- trated this in the same way we did for the case of interruption. The left hand side of the solid line is lower than that of the dotted because ... for the dotted line, which is not the case for the solid line. Also, the peaks are higher for the solid line than dotted. • The area covered by the solid li...

Ngày tải lên: 20/02/2014, 18:20