Báo cáo khoa học: HIV-1 gp41 and gp160 are hyperthermostable proteins in a mesophilic environment ppt

... the same protein sample. Reversibility data are given in Table 1. For clarity reasons, DSC thermograms and mass spectra are moved arbitrarily on the y-axis. Proteins were analyzed in 50 m M formate, pH ... supernatant and pellet were analysed by SDS/PAGE and recombinant proteins were detected by Western blot analysis using a monoclonal antibody raised against poly-histidin...

Ngày tải lên: 30/03/2014, 13:20

... Dacka for his help in running the experiment and analysing the data. References M. Argyle and J. A. Graham. 1976. The Cen- tral Europe experiment: Looking at persons and looking at objects. Environmental ... remem- bering to make a snowman and an L shape. We measured task success in two ways, correspond- ing to these two main tasks. The user’s success in the overall assembly...

Ngày tải lên: 17/03/2014, 01:20

... oxidative folding of Tim9 determines its rate of transport, and zinc may play a chaperone-like role, by initiating a conformational change of Tim9 prior to transport [27]. Recombinant proteins and ... protein folding and maturation (in particular in the ER, but also in other compartments), cellular redox regulation, ER-associated degradation, and the unfolded protein respon...

Ngày tải lên: 29/03/2014, 22:21

... 5¢-GGATCCTCACTGTG TTTCGTCAGCATC-3¢; xEIAP-F, 5¢-CATATGAGTCGC TGGTCCGTATCC-3¢ and xEIAP-R, 5¢-GGATCCTCAG GACATGAAGGCTCTAAC-3¢; xXIAP-F, 5¢-CATATGA CATGCCAGTGTCCAAAG-3¢ and xXIAP-R, 5¢-GGATC CAACTGAGCTTTGGCATTTC-3¢. To produce ... human survivin. (B) Amino-acid sequence alignment of xEIAP ⁄ XLX, xXIAP, and human XIAP. Identical and similar residues are boxed and shaded, respectively...

Ngày tải lên: 30/03/2014, 16:20

... monomeric actin and actin severing because of the rapid depolymerization that they induce [31,34]. However, jasplakinolides induce actin polymerization, stabilizing actin filaments and actin nucleation ... b-thymosins and inhibiting polymerization of G-actin and promoting depolymerization of F-actin [31,34]. Mycalolide-B and aplyronine -A inhibit polymerization of purified actin, app...

Ngày tải lên: 18/02/2014, 14:20

... Guanidinium chloride- and urea-induced unfolding of FprA, a mycobacterium NADPH-ferredoxin reductase Stabilization of an apo-protein by GdmCl Nidhi Shukla 1 , Anant Narayan Bhatt 1 , Alessandro ... Changes in functional and structural properties of FprA on incubation with increasing concentration of GdmCl at pH 7.0 and 25 °C. (A) Changes in enzymatic activity of FprA on incubation...

Ngày tải lên: 19/02/2014, 16:20

... Bromhaedia finlaysonia, Arabidopsis thaliana, Persea americana, Ipomea purpurea, Ipomea nil and Medicago sativa), three anthocyanidin synthases (Zea mays, Anthirrhinum majus and Oryza sativa), five gibberellin C20 ... doi:10.1046/j.1432-1033.2002.03108.x Several intermolecular dioxygenases, particularly those of microbial or human origin, catalyze reactions of medicinal and industrial rel...

Ngày tải lên: 21/02/2014, 03:20

... Natl Cancer Inst 100, 109–120. 77 Minakuchi Y, Takeshita F, Kosaka N, Sasaki H, Yamamoto Y, Kouno M, Honma K, Nagahara S, Hanai K, Sano A et al. (2004) Atelocollagen-mediated synthetic small interfering ... that RNA aptamers can be facilely obtained by in vitro transcription reaction and, therefore, avoid contamination by cell or bacterial products. Promising in vitro and in vivo...

Ngày tải lên: 06/03/2014, 01:23

... open reading frame of the human geminin cDNA. A probe directed against the actin mRNA served as a loading control. A blot containing total mRNA from human tumor a nd normal samples was purchased from ... (marked HeLa) was run alongside known amounts of recombinant full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng...

Ngày tải lên: 07/03/2014, 16:20

... Levin and Prince (1982), namely that gapping is acceptable only with the purely conjunc- tive symmetric meaning of and conjoining the clauses, and not with its causal asymmetric meaning (para- ... why any co- ordinated cases are unacceptable. VP-ellipsis is characterized by an initial source sen- tence, and a subsequent target sentence with a bare auxiliary indicating the...

Ngày tải lên: 08/03/2014, 07:20