Báo cáo khoa học: Mutational analysis of a type II thioesterase associated with nonribosomal peptide synthesis pdf
... AGA AGG C TEII srf D18 9A 5¢ C TTT AAC GGG CTT GCT GAT AAA AAA TGC ATA CGA GAT GCG G 3¢ C CGC ATC TCG TAT GCA TTT TTT ATC AGC AAG CCC GTT AAA G TEII srf D19 0A 5¢ C TTT AAC GGG CTT GAT GCT AAA AAA ... AAA TGC ATA CGA GAT GCG G 3¢ C CGC ATC TCG TAT GCA TTT TTT AGC ATC AAG CCC GTT AAA G TEII srf 5¢ C TTT AAC GGG CTT GCT GCT AAA AAA TGC ATA CGA GAT GCG G DD189/190AA 3¢ C CGC ATC TCG TAT GCA TT...
Ngày tải lên: 30/03/2014, 13:20
... respectively, with an A increased the rate of latency transition more than twofold. Three variants, I13 7A, V14 2A, and N15 2A, had a biphasic loss of activity, one component with a significantly faster latency ... transition rate and another component with a significantly slower latency transition rate. The activity of the three variants PAI-1 (N13 9A) , PAI-1(W14 1A) and...
Ngày tải lên: 20/02/2014, 11:20
... nm bandpass fil- ter). Data were recorded and analyzed with flowmax soft- ware from Partec. Statistical analysis of ELISA experiments Each experiment was repeated at least twice, with duplicate or ... Visai 1,2 , Fiona M. Burke 3 , Joan A. Geoghegan 3 , Matteo Stravalaci 4 , Marco Gobbi 4 , Giuliano Mazzini 5 , Carla Renata Arciola 6 , Timothy J. Foster 3 and Pietro Speziale 1 1 Depar...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant pot
... Mutational analysis of substrate recognition by human arginase type I ) agmatinase activity of the N130D variant Ricardo Alarco ´ n, Marı a S. Orellana, Benita Neira, Elena Uribe, Jose ´ R. ... blot analysis of wild -type (WT) and N130D variants of human arginase I using an antibody raised against E. coli agma- tinase (AUH). The relative molecular masses of the protein ma...
Ngày tải lên: 16/03/2014, 12:20
Báo cáo khoa học: "Morphological Analysis of a Large Spontaneous Speech Corpus in Japanese" pptx
... TOC(0)(Beginning) Kanji, Hiragana, Number, Katakana, Alphabet (5:5) 19 TOC(0)(End) Kanji, Hiragana, Number, Katakana, Alphabet (5:5) 20 TOC(0)(Transition) Kanji→Hiragana, Number→Kanji, Katakana→Kanji, (25:25) 21 ... estimated a low probability, as will be shown later. The recall of unknown words was lower than that of known words, and the accuracy of automatic mor- phological analysi...
Ngày tải lên: 17/03/2014, 06:20
Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
... CfrI 5¢R6 2A 5¢-CTG CAT GTG CTG GTA GAG gcc TAC GAT GTA TTC CGC ACG-3¢ AatI 3¢R6 2A 5¢-CGT GCG GAA TAC ATC GTA ggc CTC TAC CAG CAC ATG CAG-3¢ AatI 5¢R6 7A 5¢-GGT AGA GAG ATA CGA TGT ATT Cgc gAC GTT GTT TAT ... GTT TAT CTA TGA AAA GC-3¢ NruI 3¢R6 7A 5¢-GCT TTT CAT AGA GAA ACA ACG Tcg cGA ATA CAT CGT ATC TCT CTA CC-3¢ NruI 5¢W202L 5¢-CAG GCC GCT CTC AAC TAc Tta AGC GAC TAT CTG GAA GCC-3¢ A...
Ngày tải lên: 17/03/2014, 11:20
Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt
... CAG-3¢ 5¢-GCCCTGACAAATTTG GGAGTGCTACTTTATGGCTG-3¢ 5¢-GTAGCACTCCCAAATTTGTCAGGGCAATAGACG C ⁄ D XhoI, SacI mrs2-J11 1021, 1024 and 1026 Glu341 + Glu342 to Lys 5¢-GCATTTTATGGTATGAATTTAAAGAATTTCATC AAGAAAAGTGAATGGG-3¢ 5¢-CCCATTCACTTTTCTTGATGAAATTCTTTAAAT TCATACCATAAAATGC-3¢ A ... 5¢-GAGATCCATTAGATGAACTATTAGAAAAC AAAGATGATTTAGCAAACATGTACTTGACA-3¢ 5¢-TGTCAAGTACATGTTTGCTAAATCATCTTTGTTT TCTAATAGTTCA...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khoa học: Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA pot
... FEBS DNA-binding properties of mutant proteins as measured by a gel shift assay A structural alignment of core subdomain II of human topoisomerase I with the POU homeodomain of Oct-1 indicated that ... Coomassie blue stain- ing and photographed using the AlphImager IS-2200 (Alpha Innotech, San Leandro, CA, USA) digital imager. Generation of nicked and linear DNA Unlabeled an...
Ngày tải lên: 29/03/2014, 22:21
Báo cáo khoa học: Proteome analysis of a rat liver nuclear insoluble protein fraction and localization of a novel protein, ISP36, to compartments in the interchromatin space pptx
... form an internal nucleoskeleton as well as a peripheral lamina in human cells. J Cell Sci 108, 635–644. 10 Jagatheesan G, Thanumalayan S, Muralikrishna B, Rangaraj N, Karande AA & Parnaik ... pro- tein that associates with nuclear matrix. DNA Cell Biol 17, 849–858. 24 Lee JY, Nakane Y, Koshikawa N, Nakayama K, Hayashi M & Takenaga K (2000) Characterization of a zinc finger prot...
Ngày tải lên: 30/03/2014, 20:20
Tài liệu Báo cáo khoa học: Fluorescence analysis of the Hansenula polymorpha peroxisomal targeting signal-1 receptor, Pex5p pdf
... at the emission maximum) with that of the standard N-Ac-Trp- NH 2 normalized to the same absorbance at 295 nm. A value of 0.13 was used for the quantum yield of the standard [16]. Quenching of ... investigate the binding of the PTS1 peptide and corre- late the data obtained from Trp fluorescence analysis, a molecular model of HpPex5p was built using the programs WHAT IF [1...
Ngày tải lên: 21/02/2014, 00:20