Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... we describe the isolation of Xenopus HIVEP1 ,aswellas partial cDNAs of HIVEP2 and -3. Analysis of the tem-poral and spatial expression of the XHIVEP transcriptsduring early embryogenesis revealed ... combinations of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective3¢ ends and included 3¢-UTRs and poly(A)-tails.A ... analyze the function of XHIVEP in the context of Xenopus embryogenesis (data not shown).However, the interpretation of the in vivo role of XHIVEPwas not conclusive as the activator and repressor fusionconstructs...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... comprising the two sugarbinding pockets in the pulchellins (Fig. 3).In order to compare the similarity of the A- and B-chains of the four isoforms, a pairwise alignmentwas performed and the values of ... Taken together with the inability of P III and P IV to inhibit hemagglutination in vitro in the presence of this sugar, these data suggest that the bind-ing and uptake of these two isoforms does ... on the left indicate the Mrvalues of the standards in thousands. Lanes 2–9, 5 lg of peakP I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec-tively, in the presence (lanes 2–5)...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... samples, and were used for the quantification of the enzymatically formed H2S.Measurement of pyruvate formation The activity of the purified d-CDes enzyme was followedthrough the measurement of the ... and decreased at the beginning of the light cycle. The d-CDes gene expression or the stability of the d-CDesmRNA was negatively regulated by light (Fig. 6A). The Western blot results using the ... cycle and the parts above ground were harvestedevery 4 h and frozen in liquid nitrogen. The d-CDesmRNA levels increased at the end of the light period,reached a maximum at the end of the dark...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... detailedanalysis of the 12F-11 paratope and assessment of the varying contributions of CDR and framework regionsclearly requires definitive structural data; this analysis iscurrently in progress. The isolation ... (1999)Mutational pattern of the nurse shark antigen receptor gene (NAR) is similar to that of mammalian Ig genes and to sponta-neous mutations in evolution: the translesion synthesis model of somatic hypermutation. ... Analysis of the binding of the Fab fragment of monoclonal antibody NC10 to influenzavirus N9 neuraminidase from tern and whale using the BIAcorebiosensor: effect of immobilization level and flow...
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Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot
... intensity of the bandsmay be the result of a different copy number of the genes in the hybridizing fragments. If so, the number of the bands donot represent the family size. Thus, the mlp gene family ... of the mlp gene. Nevertheless, taking into account the possible size of the mlp gene family and the number of gene variants considered in the construction of the M1 and M2 primers, the error maybe ... site.Sizing of the family of toxic lectin genes in mistletoeAssuming that there is a single copy of the ml2p gene perhaploid genome, then it follows from the ratio of three gene variants that the size...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... experimentally. Indeed, the presence of Arg213 along with the proximity of the chargerelay Asp260 within the active site could be responsible for the modification of the catalytic properties of the catalytichistidine. ... [23].Results and DiscussionCloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acidprotein with a molecular mass of 31.7 kDa and acalculated pI of 4.53 and belongs to the ... avor the displacement of the equilibriumtoward the neutral state of His290.To attest this hypothesis, an Arg213Ala mutant has beenexpressed and characterized. The specific activity of the Ala213...
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Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot
... role for these residues on the catalysis and zinc ions, respectively. These effects may be necessary eitherfor the activation of the catalytic water molecule or for the stabilization of the substrate–enzyme ... examined the V. alginolyticus PepD protein. In the present study, wepresent the cloning and expression of the V. alginolyti-cus pepD gene, the purification and biochemical char-acterization of the ... activity [33].These results indicate the importance of these residuesin the stability of di-zinc binding and suggest thatthese residues are essential for the enzymatic activity of the PepD.To...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... considering the oxidation of the substrate byT. reesei TYR2. The closer to the hydroxyl group of phenol the amino group was, the slower was the oxida-tion of the substrate. For most of the substrates ... these states are alternated, has beenproposed [16]. The met state is the resting state of the enzyme, and in the absence of substrate about 85–90% of the enzyme is in this state [16]. Both the ... available, and will enable more detailed analy-sis of the exact reaction mechanisms. The tyrosinasestructure, wherein the active site was located at the bottom of a large vacant space and one of the...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... stresses. The exist-ence of a large family of CIPK and CBL genes hasbeen reported in Arabidopsis and rice [15]. However, the details of the role of these proteins and the inter-Stress-induced CIPK ... showing the nuc-leus, and Fig. 7C, F, K and N are the merged images of A and B, D and E, I and J, and L and M, respect-ively.DiscussionNature has developed many pathways for combating and tolerating ... wasperformed using PsCIPK gene- specific internal prim-ers. These fragments were then cloned and sequenced(data not shown). The same size and sequence of the genomic fragment of PsCIPK and the cDNA showthat...
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Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... evidence for existence of twodistinct HSF1 isoforms in rainbow trout and the formation of heterotrimers of these isoforms in vitro.Materials and methodsCell culture and animalsRainbow trout ... 10.0;GAPEXT,0.05;GAPDIST,8;MAXDIV,40;ENDGAPS,off;NOPGAPS,off;NOHGAPS,off.Graphicaloutput of the bootstrap figure was produced by the programTREEVIEW. Isolation of RNA and RT-PCR analysisTotal RNA was isolated from RTG-2 cells and ... weredetected in the lanes containing HSF1a–HA (Fig. 7A, lanes1 and 3). The apparent molecular masses of the bands were 200 kDa and 70 kDa. These molecular masses corres-pond to the sizes of an HSF1...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Phát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngThiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Nguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)BÀI HOÀN CHỈNH TỔNG QUAN VỀ MẠNG XÃ HỘIChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ