Báo cáo khóa học: Conformational changes of Newcastle disease virus envelope glycoproteins triggered by gangliosides pdf

... Conformational changes of Newcastle disease virus envelope glycoproteins triggered by gangliosides Laura Ferreira, Enrique Villar and Isabel ... structural changes [14,16,19,20]. The exposure of hydrophobic segments of NDV envelope proteins triggered by gangliosides was tested by measuring binding to bis-ANS. Initially, the effect of increasing ... indi...

Ngày tải lên: 30/03/2014, 13:20

... Polarity of AOT micellar interfaces: use of the preferential solvation concepts intheevaluationoftheeffectivedielectricconstants.J. Phys. Chem. 94, 5337–5341. Ó FEBS 2004 Conformational changes of bLG ... 6 M GdnHCl(4)andatatemperature(T )of7 5°C(5).Inset: Wavelengths of maximum emission (j) and fluorescence quantum yields (s) as a function of x 0 andinwater. Ó FEBS 2004 Conforma...

Ngày tải lên: 07/03/2014, 15:20

... the SV40 genome in virus infected cells also obeys the oxygen-dependent regu- lation [6,7]. Reoxygenation of virus infected cells after several hours of hypoxia triggers a burst of hypoxically accumulated ... analyses of growing daughter strands A fast increase of the DNA synthesis rate either reflects release of replicon initiations or stimulation of elongation, or both. To...

Ngày tải lên: 21/02/2014, 00:20

... experimental data consisted of repeating sequences of measurements of the parallel and perpendicular polarized emission fluorescence decays of the reference compound (three cycles of 20 s), the protein ... contribution of each correlation time to the total anisotropy decay. The anisotropy decay parameters were estimated by simultaneous analysis of I || (t) and I ^ (t). The good...

Ngày tải lên: 07/03/2014, 21:20

... understanding of the catalytic mechanisms of human HSDs that are implicated in the development of steroid-dependent forms of cancer, in polycystic kidney disease, in the regulation of blood pressure, ... Lanis ˇ nik Riz ˇ ner 2 1 Department of Food Science and Technology, University of Ljubljana, Slovenia 2 Institute of Biochemistry, University of Ljubljana, Slovenia Protei...

Ngày tải lên: 16/03/2014, 13:20

... Binding of ATP to DnaK promoted quenching of the single Trp residue of DnaK and a blue-shift of its emission maximum (Fig. 4B, upper traces). In addition to these spectral changes, reduction of the ... that of DnaK. The twofold reduction of the K SV values estimated for mtHsp70 both in the absence and the presence of ATP (Table 2) supports the existence of differences in th...

Ngày tải lên: 16/03/2014, 22:20

... nature of the alteration in dynamic and how it is linked to the small observed changes of orientation of helix G [7,13] is unclear, however, it is possible that an increase in intensity of this ... Rink T, Pfeiffer M, Oesterhelt D, Gerwert K & Stenh- off HJ (2000) Unraveling photoexcited conformational changes of bacteriorhodopsin by time resolved electron paramagnetic r...

Ngày tải lên: 23/03/2014, 13:20

... (apoNgb) as a result of the formation of an inter- molecular disulfide bond. The preparation of apoNgb was obtained from the corresponding holoprotein by the removal of heme by reverse-phase HPLC ... to substrate ratio of 1 : 100 (by weight). At time intervals, aliquots were taken from the reaction mixture and proteolysis was stopped by the acidification of the solutions by...

Ngày tải lên: 30/03/2014, 01:20

... detailed sequence of zinc- coordination changes during catalysis and the mode of inhibition of anions such as fluoride and phosphate. Experimental procedures Purification of SGAP The cloning of the SGAP ... substrates by thermolysin. Biochemistry 33, 6516–6523. 58 Yang JJ & Van Wart HE (1994) Kinetics of hydrolysis of dansyl peptide substrates by thermolysin: analysis o...

Ngày tải lên: 19/02/2014, 02:20

... exhibit a wide range of structural diversity produced by a sequence of biosynthetic reactions. Cyclization of oxidosqualene is the ini- tial origin of structural diversity of skeletons in their ... the production of not only flavonoid but also of triterpene saponins is induced by elicitation as mentioned above, triterpene hydroxylase is one possible function of CYP93E1. Fee...

Ngày tải lên: 19/02/2014, 07:20