Báo cáo khóa học: High level cell-free expression and specific labeling of integral membrane proteins doc

... advan- tages of the cell-free expression of IMPs were the high level production of insoluble protein and the efficient selective labeling. This is the first report of the solubilization of SugE, YfiK and ... folded conformation of the proteins. Keywords: amino acid specific labeling; cell-free expression; integral membrane proteins; multidrug transporter; sol...

Ngày tải lên: 30/03/2014, 13:20

... QQFPQQQ, QQSPEQQ, QQSPQQQ, QQYPQQQ and QQPPQQ. The serum of patient two had specific IgE antibodies to QQIPQQQ, QQFPQQQ, QQSPEQQ, QQSPQQQ and YQQYPQQ. The serum of patient three had specific IgE antibodies ... shown in Fig. 3 lane 2 a high level of expression of recombinant protein, designated rO5GC, was obtained. The x-5 gliadin purified from wheat flour is slightly soluble...

Ngày tải lên: 20/02/2014, 01:20

... tested VGSC isoforms. Results Screening of peptides for robustness of the slowing of the inactivation process both in mammalian isoforms and in one insect isoform As only a limited amount of each compound ... analogs of PMTX and the eight VGSC isoforms. A maximal concentration of 46 l M was used (n = 4 for each data point). See [17] and Experimental procedures for details o...

Ngày tải lên: 15/03/2014, 10:20

... M. Nicholson and G. F. King, and DP0774245 to G. F. King) and Australian Postgraduate Awards to S. J. Gunning and M. J. Windley. Requests for samples of J-ACTX-Hv1c and ⁄ or the expression system ... bonds and b-strands are shown in red and cyan, respec- tively. (D, E) Surface representation of J-ACTX-Hv1c (D) and ChTx (E), highlighting the primary pharmacophore residues...

Ngày tải lên: 16/03/2014, 06:20

... & Dia- mandis EP (2003) Human kallikrein 13: production and purification of recombinant protein and monoclonal and polyclonal antibodies, and development of a sensi- tive and specific immunofluorometric ... development of highly specific inhibitors for hK14. Using two different backbones, we developed four recombinant inhibitors, two of which demonstrated high specifici...

Ngày tải lên: 30/03/2014, 11:20

... Hellman, L. (1993) Expression of a mast cell tryptase in the human monocytic cell lines U-937 and Mono Mac 6. Scand. J. Immunol. 38, 359–367. Ó FEBS 2003 Tissue -specific expression of bovine tryptases ... cloning, tissue specific expression and characterization of the lung isoform Alessandra Gambacurta 1 *, Laura Fiorucci 1 *, Paolo Basili 1 , Fulvio Erba 1 , Angela Amoresan...

Ngày tải lên: 17/03/2014, 09:20

... relative expression level of the GFP-tagged proteins is indicative of that of the equivalent untagged proteins. Lipid anchoring of C-Vrp1p bypasses the requirement for the LBD for endocytosis and growth ... but we expect that the relative expression level of the tagged proteins is indicative of that of the equival- ent untagged proteins. To assess the function...

Ngày tải lên: 18/02/2014, 16:20

... data- base of Web ngrams – snippets of Web text (of one to five words) that occur on the web with a fre- quency of 40 or higher (Brants and Franz, 2006). Each is likely a literal description of a real ... comple- ment of adjectival properties used by Veale and Hao (2007), we harvest all instances of the patterns “as ADJ and * as” and “as * and ADJ as” from Google, notin...

Ngày tải lên: 20/02/2014, 05:20

... Department of Biochemistry and Molecular Biology, School of Biomedical and Chemical Science, The University of Western Australia, Crawley, Australia The predominant source of the main form of vitamin ... alternate pathway of vitamin D3 metabolism Andrzej Slominski 1 , Igor Semak 2 , Jordan Zjawiony 3 , Jacobo Wortsman 4 , Wei Li 5 , Andre Szczesniewski 6 and Robert C. Tuckey...

Ngày tải lên: 07/03/2014, 21:20

... Montan ˜ oLF&Lo ´ pez-Marure R (2005) Dehydroepiandrosterone inhibits the prolifera- tion of HUVEC by enhancing the expression of p53 and p21, restricting the phosphorylation of RB, and is androgen- and estrogen-receptor ... estrogen and androgen, because use of antagonists to androgen and estrogen receptors (flutamide and ICI 182,780, respectively), and letrozol, a...

Ngày tải lên: 16/03/2014, 00:20