Báo cáo khóa học: FTIR spectroscopy shows structural similarities between photosystems II from cyanobacteria and spinach potx

... FTIR spectroscopy shows structural similarities between photosystems II from cyanobacteria and spinach Andre ´ Remy 1 , Jens Niklas 1 , Helena Kuhl 2 , ... plants and cyanobacteria, and FTIR difference spectroscopy allows prediction of strong structural similar- ities between these photosystems, even though a structural model of higher plant PSII is ......

Ngày tải lên: 30/03/2014, 13:20

... (most from Sigma Chemical Co., St Louis, MO, USA) and were used without further purification. Restriction enzymes, and enzymes and reagents for PCR were from Promega. The plasmid pBluescript II K(+), ... II. Results and Discussion General properties of the wild-type, His120Asn, His145Asn and Asn149Asp variants of arginase II Purified wild-type, His120Asn and His145Asn vari...

Ngày tải lên: 20/02/2014, 02:21

... types I and III. Purified MMP-9 was able to cleave the soluble, monomeric forms of native collagen types I and III at 37 °C and 25 °C, respectively. Activity against collagens I and III was abolished ... types I, II and III [5–7] with a sub- strate preference of type III > type II > type I [6]. The data shown here indicate that MMP-9 also has a clear substrate preference fo...

Ngày tải lên: 23/03/2014, 09:21

... calcium- binding loops (Fig. 2). I II III IV I′ II III′ IV′ annexin A2 annexin A2 Ca 2+ I II III IV I′ II III′ IV′ I II III IV I′ II III′ IV ′ annexin A1 annexin A1 helix II I movement annexin ... (residues 1–105) and the second portion formed from helices V–VIII (residues 119–217). The ribbon diagram shows helices I and V (red), II and VI (yellow), III an...

Ngày tải lên: 18/02/2014, 14:20

... (proteinases from Vibrio alginolyticus, Vibrio cholerae, Kytococcus sedentarius and Streptomyces coelicolor) and thermophilic origin (aqualysin I from Thermus aquaticus and proteinase from Thermus ... the highly homolog- ous proteinases from Vibrio alginolyticus and Vibrio cholerae, but absent in the thermophilic proteinase from Thermus Rt41a and aqualysin I from Thermus...

Ngày tải lên: 19/02/2014, 16:20

... benefit for NL understanding and/ or gener- ation. But the work to date surely shows the benefit of an approach that narrows the gap between dis- course syntax and semantics and that of the clause. ... one of its argu- ments coming structurally. The other argument is presupposed and thus able to come from across a structural boundary, as in (1) a. On the one hand, John lo...

Ngày tải lên: 20/02/2014, 18:20

... 540 cm )1 (amide II) [15]. The 1450 and 1400 cm )1 bands arise from the side chain o f p roteins [ 15], but the C –H bend ing vibration of fatty acids at 1467 and 1450 cm )1 [3] and the carboxylate vibration ... uperimposed. Absorptions between 1300 and 900 cm )1 arise mainly from phosphate associated with nucleic acids, i.e. DNA and RNA. The a bsorption bands a t 1245 a nd 1...

Ngày tải lên: 21/02/2014, 03:20

... 208–560 and 208–1102 were amplified with primers containing restriction sites for HindIII and XbaIas 5¢- and 3¢-overhangs, respectively. The PCR products obtained were ligated into pCR2.1-TOPO and ... Coomassie stained and major bands were excised and eluted in order to perform N-terminal sequencing. Tandem affinity purification and mass spectrometric analysis of USP7 N-terminal a...

Ngày tải lên: 07/03/2014, 05:20

... (–) were introduced to increase similarities. Toxins sequences were obtained from data bank and the abbreviations stand for: AaH, Androctonus australis Hector; Amm, Androctonus mauretanicus mauretanicus;Bj,Buthotus ... lines: TE671 (from human cerebellar medulloblastoma), COS7 (from monkey kidney fibro- blasts), GH3 and cerebellum granular ce lls from r at, using the technique descr...

Ngày tải lên: 07/03/2014, 16:20

... struc- tures of wild-type and C69A mutant TcRpiB, with and without bound substrate and inhibitors, allowed us to study in detail the interactions between the enzyme and bound ligands, as well as small ... 1. Sequence comparison, structural analysis and figure preparation Similar sequences ⁄ structures were identified using blast [37], pfam [8] and dali [38], obtained from GenB...

Ngày tải lên: 15/03/2014, 00:20