Báo cáo khóa học: Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans docx

... Mazoch et al. (Eur. J. Biochem. 271) Ó FEBS 2004 Isolation and biochemical characterization of two soluble iron(III) reductases from Paracoccus denitrificans Jir ˇ ı ´ Mazoch 1 , Radek Tesar ˇ ı ´ k 2 , ... Republic Two soluble enzymes (FerA and FerB) catalyzing the reduction of a number of iron(III) complexes by NADH, were purified to near homogeneity from...

Ngày tải lên: 30/03/2014, 13:20

... FEBS 3579 characterization and evaluation of HYD Js from Jannaschia sp. CCS1. Characterization of HYD Js To explore the possible cause of the higher specific activity for conversion of d,l-p-HPH ... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between sub...

Ngày tải lên: 18/02/2014, 08:20

... jellyroll domain formed by two b-sheets and a distal domain formed by insertions between the strands of the jellyroll domain (Fig. 3). Antiparallel b-sheets made up of strands CHEF and BIDG pack against ... is formed by an insertion of  230 residues (residues 133–367) between strands D and E and a smaller insertion of  50 residues (residues 404–458) between strands F and...

Ngày tải lên: 19/02/2014, 06:20

... reagents were of analytical grade. Isolation and purification of lamjapin from kelp The fresh tender leaves of kelp were freeze-dried and powdered in liquid nitrogen. Thirty grams of the finely ground ... of action of RIP and for analysis of the chemistry of recognition of RNA by RIP [22,23]. As shown in Fig. 5, ricin could deadenylate A20 of SRD RNA, the site corr...

Ngày tải lên: 08/03/2014, 10:20

... types of plastids. The Ndh complex associates in homodimers and dissociates in membrane and soluble subcomplexes; isolation of the Ndh complex by BN / PAGE and Tricine / PAGE Intact MS and BS ... exists as a monomer and dimer and splits into a membrane and soluble subcomplexes. Separation of the Ndh complex by CN / PAGE (1D) and Tricine / PAGE (2D) and CN / PAGE...

Ngày tải lên: 23/03/2014, 13:20

... transfer of the c-phosphate group from ATP to AMP, releasing two molecules of ADP. This study describes cloning and functional characterization of the gene encoding AK2 from a genomiclibrary of Leishmania ... transfer of the c-phos- phate group from ATP to AMP, releasing two molecules of ADP [1]. Different isoenzymic forms of AK are involved in maintenance of con...

Ngày tải lên: 21/02/2014, 00:20

... limited set of enzymes involved in amino acid synthesis of Ser from Gly and Ala from Cys and conversions between Asp and Asn and between Glu and Gln [49]. Serine metabolic pathways are often absent ... Identification and characterization of two isoenzymes of methionine c-lyase from Entamoeba histolytica: a key enzyme of sulfur-amino acid degradation in an anaer...

Ngày tải lên: 19/02/2014, 13:20

... DNA and RNA were performed as described previously [57]. Isolation and cloning of the SynMGST and E.coliMGST The coding sequence for the SynMGST, corresponding to the complementary strand of the ... al. Characterization of MAPEG members FEBS Journal 272 (2005) 1688–1703 ª 2005 FEBS 1699 BamHI and EcoRI and then the reformatted BamHI-EcoRI fragment was subcloned into BamHI...

Ngày tải lên: 19/02/2014, 17:20

... oligomeric states of CR I–II and Calb I–II are an indication of different organizations of EF-hands within full-length CR and Calb. CR I–II and Calb I–II may have different structures CR I–II and Calb ... to dimerize and both EF-hands of CR I –II bind calcium. We conclude that the significant structural and biochemical differences between CR I–II and Calb I–II are relat...

Ngày tải lên: 22/02/2014, 07:20

... signal trans- ducer and activator of transcription 3 (STAT3), and hematopoietic progenitor kinase 1-interacting protein of 55 kDa (HIP-55). Using both MS and MALDI- TOF ⁄ TOF MS tryptic mass fingerprinting, ... percentage of breast tumors in the early stage of the disease, contributing to the transformation of cells and also to the further progression of breast cancer [42]....

Ngày tải lên: 06/03/2014, 22:21