Báo cáo khóa học: Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico, in vitro and in vivo pot

... 585–596. 134 S. Andre ´ et al.(Eur. J. Biochem. 271) Ó FEBS 2003 Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GlcNAc in silico , in ... histopatho- logical and in vivo procedures. The question of whether introduction of the bisecting GlcNAc changes the ligand...

Ngày tải lên: 30/03/2014, 13:20

... the A C B Fig. 2. Effect of the binding of ATP ⁄ Mg 2+ to 2-Cys Prx on the fluo- rescence emission of extrinsic and intrinsic chromophores. (A) Sen- sitivity of the extrinsic probe ANS. Binding of ANS to 2-Cys ... information about the polarity of local environments surrounding either extrinsic probes that bind to proteins or intrinsic fluorophores buried in the protein interior...

Ngày tải lên: 18/02/2014, 17:20

... residues include Fuc, in addition to one reducing terminal 4-linked Glc, four Gal (two terminal, one internal 3-linked and one 3,6-disubstituted) and three GlcNAc (one 3,4-disubstituted and two 3-linked). ... modifying protein function. One of the early demonstrations of the role of carbo- hydrates in recognition was binding of the in uenza virus to red blood cells via si...

Ngày tải lên: 19/02/2014, 12:20

... bias introduced by a possible shift of the charge state distribution caused by ligand binding. The dimeric protein forms were disregarded in the determination of binding constants because of their low ... of MS in protein analysis because of its inherently ultra-high mass-resolving power and mass accuracy. For instance, protein ligand [57], protein–carbohydrate [58,59...

Ngày tải lên: 07/03/2014, 17:20

... Determination of the reopening temperature of a DNA hairpin structure in vitro Xuefeng Pan Institute of Microbiology, The Chinese Academy of Sciences, Beijing, China A novel method, ... the orientation of the insert in M13mp19 was determined by PstI digestion. Single-stranded M13 DNA was prepared as described by Sambrook et al. [18]. Annealing of the primer–DNA templat...

Ngày tải lên: 23/03/2014, 13:20

... FAD/NADPH- and FMN-binding domains of NR1 have been expressed and purified and their redox properties studied by stopped-flow and steady-state kinetic methods, and by potentiometry. The midpoint reduction ... kinetic properties of NR1, and as we have shown for CPR and P450 BM3 (23,24,28) the properties of the flavin binding domains of NR1 are likely to mimic the r...

Ngày tải lên: 23/03/2014, 21:20

... of syntactic analysis of Estonian using Constraint Grammar. Constraint Gram- mar framework divides parsing into two different modules: morphological disam- biguation and determination of ... Proceedings of EACL '99 Determination of Syntactic Functions in Estonian Constraint Grammar Kaili Mfifirisep Institute of Computer Science University of Tartu Liivi 2,...

Ngày tải lên: 24/03/2014, 03:21

... determined by adding XylE and TodF stepwise to solutions containing known amounts of catechol and deter- mining the resulting UV absorbance. Complete cleavage of catechol by XylE and the amounts of ... albumin as standards. SDS ⁄ PAGE was performed and stained with Coomassie blue according to established procedures [28]. The Bench- Mark TM Protein Ladder (Invitrogen) contain...

Ngày tải lên: 30/03/2014, 15:20

... protein (1B7F_1) and KH1 domain of Poly C-binding protein-2 were both ranked first out of 256 sequences, whereas KH domain 3 of hnRNP K (1ZZI), RRM of U2B¢ protein (1A9N) and RRM 4 of Polypyrimidine ... model, complexes containing two RNA-binding domains were divided into independent structures (e.g. 1CVJ_1 and 1CVJ_2 represent the first and sec- ond Poly A binding protein dom...

Ngày tải lên: 18/02/2014, 16:20

... 697 in the presence of spermine (Fig. 5, lane 1), spermidine or monovalent cations (data not shown). On the other hand, it is diminished by refolding of tRNA in 10 m M Tris/HCl pH 7.0 containing ... stringency of each aminoacyl- tRNA synthetase and the number of type 2 tRNAs in particular cellular compartment, it was shown that tRNA discrimination by SerRS and LeuRS in...

Ngày tải lên: 19/02/2014, 12:20