Báo cáo khóa học: UDP-galactose 4-epimerase from Kluyveromyces fragilis Evidence for independent mutarotation site pdf

... from Lactococcus lactis. J. Biol. Chem. 277, 20854–20861. 68 A. Brahma and D. Bhattacharyya (Eur. J. Biochem. 271) Ó FEBS 2003 UDP-galactose 4-epimerase from Kluyveromyces fragilis Evidence for ... residue for the activity of UDP-galactose 4-epimerase from Kluyver- omyces fragilis. J. Biol. Chem. 267, 11709–11713. 34. Mukherjee, S. & Bhaduri, A. (1986) UDP-glucose...

Ngày tải lên: 30/03/2014, 13:20

... between the examples gathered from parallel texts and the evaluation data for the two SENSEVAL tasks. This gave a set of 6 nouns for SENSEVAL-2 and 9 nouns for SENSEVAL- 3. For each noun, we gathered ... We performed 10-fold cross- validation on the training data to assign values to . We then applied the PAV algorithm to obtain values for . To obtain the calibrated probability est...

Ngày tải lên: 08/03/2014, 02:21

... We have therefore examined extracts from Val135 and Pro190 MEL cells for the integrity of eIF4GI and eIF4B following the shift to 32 °C. Figure 3 shows that, although the effect was variable from one ... transferred from 38 °Cto32°C and incubated for the times shown. Rates of protein synthesis per 10 5 cells were then measured by pulse-labeling the cells with [ 35 S]methionine (10 l...

Ngày tải lên: 23/03/2014, 18:20

... NaOH from an anion exchange column. The retention time for trehalose was 2.65 min. The y-axis shows relative detector units from pulsed chromatography. Table 3. Unidentified peaks of high-performance ... 95 °C for 60 min the homogenate was centrifuged (20 000 g at 4 °C for 15 min) and the supernatant was used for carbohydrate analysis. Water-soluble carbohydrates were determined by...

Ngày tải lên: 30/03/2014, 04:20

... algorithm for S-attributed grammars. In Section 4, we consider the conditions on the underlying grammar under which the extended algorithm may be guaranteed to terminate for each input. For the ... case. For the S-attributed case, however, this may happen only if the underlying grammar is ~nfinitely ambiguous or, equivalently, if it has cycles Or derivations of the form A~+A,...

Ngày tải lên: 01/04/2014, 00:20

... eluate was then assayed for both epimerase and mutarotase activity. Results Purification of UDPgalactose 4-epimerase The purification procedure for UDPgalactose 4-epimerase from S. cerevisiae is ... gal10-deficient strain PJB5 (henceforth called transformed PJB5) by following the method of Gietz et al. [19]. To test for complementation of the gal10 mutant, transformedPJB5cellsweregro...

Ngày tải lên: 19/02/2014, 12:20

... by genes that code for the major cellulosomal cellulase Cel48F and nine other dockerin-containing enzymes [15]. Cellulases encoded by the cip-cel cluster are essential for the formation of efficient ... fractions, except for F1, which was found to be approximately fivefold more active than the others. The cellulosomes present in fractions F1 and F6 therefore have the most efficient enzymes...

Ngày tải lên: 18/02/2014, 08:20

... available evidence for EcMalP defines a range of plausible pK a values for CcStP because residues interacting with the 5¢-phosphate group in EcMalP are completely conserved in CcStP. Log k syn for the ... group of GPs from plants and microorganisms. We therefore asked the question, what novel information might be gained by applying the same type of reconstitution experiments describe...

Ngày tải lên: 19/02/2014, 16:20

... computation of prefix probabilities for TAGs is based on transforma- tions of equations. Here we informally discuss the general ideas underlying equation transfor- mations. Let w = ala2 an ... consider is proper. That is, for each N such that label(N) • NT, ¢(t, N) = 1. tE.AU{nil} For each non-leaAf node N we construct the string cdn(N) = N1 Nm from the (ordered) list of...

Ngày tải lên: 20/02/2014, 18:20

... (a probe for hydrophobic sites) and is not retained by the phenyl Sepharose column (a ligand for phenolic- recognizing sites and for hydrophobic sites in general). These results differ from those ... components’ from two other PHs. The N-terminal sequence of PHI from A. radioresis- tens S13 is identical (from residue number 3) to the sequence of the corresponding component of PH...

Ngày tải lên: 21/02/2014, 00:20