Báo cáo khóa học: Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions ppt

... and increase the flexibility of the enzyme active site. The increased flexibility in the substrate-binding site leads to the increase in K m , i.e. a decrease in the affinity of theenzymeforthesubstrate.Thedecreaseink cat , however, ... strongly indicate that the anion -induced changes in the activity of NADH oxidase are due to a change in...

Ngày tải lên: 30/03/2014, 13:20

... Non-specific inhibition of metalloprotease activity can readily be achieved by chelating agents that bind to metal cations required in the active site of the enzyme. The observed inhibition of MA on thermolysin ... quenching of the 7-methoxycoumarin-4- acetyl (MCA) group by the 2,4-dintrophenyl group (DNP). For the above, 15 lL of protein extract or the con...

Ngày tải lên: 14/02/2014, 14:20

... molecules of the bleomycin anti- biotic within two crevices formed at the interface of the domain swapped dimer [46]. Equally intriguing is the T7 endonuclease I, representing one of the most striking ... two distinct functions of TorD with the folding of TorA and possible interactions of both proteins with the MGD biosynthetic apparatus [12]. The concentration of...

Ngày tải lên: 16/02/2014, 09:20

... [25,28,29]. The molecular mechanism of BASP1 activity presum- ably involves the regulation of actin cytoskeleton dynamics through either direct interaction with actin- binding proteins [30] or modulation ... examined using the electrophysiological recording technique. The addi- tion of BASP1 to the solution in the cis-compartment resulted in the spontaneous incor...

Ngày tải lên: 28/03/2014, 23:20

... any other kinase structures that we examined. As a result of the presence of the flap, the adenosine ring of ATP is bur- ied in a deeper pocket in the kinase domain of Rio1 than in Rio2. The flap ... seen in the electron density, emphasi- zing the flexibility of this region (Fig. 3A,B). Another distinguishing feature of the Rio1 kinase domain is a conserve...

Ngày tải lên: 30/03/2014, 20:20

... defined, for in nitesimally small changes, as the percentage change in the steady-state rate of the pathway divided by the percentage change in the enzyme activity caus- ing the flux change. The FCCs ... significant production of P i in the vicinity of the ATPase within the matrix. CYPD ablation or its inhibition by cyclosporin A increases the rate...

Ngày tải lên: 14/02/2014, 19:20

... that the CT-extension blocks some of the substrate-binding unprimed subsites including the speci- ficity-determining subsite (S2) of the enzyme and thereby partially occludes accessibility of the ... docked manually into the unprimed subsites of Erv-C with Leu23 in the S2 pocket, considering the specificity of Erv-C towards a Leu residue [17]. The mode of inter...

Ngày tải lên: 14/02/2014, 14:20

... Nota- bly, in the presence of the neurotoxin, there was a delay in the lag time for fibrillation. The lag time increased from 74.9 h in case of a -synuclein alone to 86.8 and 93.6 h in the presence of ... 1B shows the pattern seen after the development of the blot. With increase in time of incubation, the intensity of the band for the monomeric pro...

Ngày tải lên: 14/02/2014, 19:20

... vitro activity of acyl-CoA:ergosterol acyltransferase was determined in an are1 deletion background. The enzyme activity in the ste20D strain and the cla4D strain was indistinguishable from that in the ... arginine at the extreme N-terminus, is targeted for degradation by the enzymes of the N-end rule. A pro- tein–protein interaction therefore results in no...

Ngày tải lên: 18/02/2014, 13:20

... L268 may in uence the conformational freedom of the sub- strate, favoring a reactive conformation. In other enzymes, the role of pressing the nicotinamide against the flavin may be carried out by other ... alanine mutation of this enzyme reduced the flavin binding energy by 0.5 kcalÆmol )1 [50]. In pea FNR, the sulfhy- dryl group of C266 is 6.17 A ˚ from the...

Ngày tải lên: 18/02/2014, 17:20