Báo cáo khoa học: Replacement of helix 1¢ enhances the lipid binding activity of apoE3 N-terminal domain pot

... al. apoE3- NT domain lipid binding FEBS Journal 273 (2006) 558–567 ª 2006 The Authors Journal compilation ª 2006 FEBS 567 Replacement of helix 1¢ enhances the lipid binding activity of apoE3 N-terminal ... wild-type apoE3- NT. The results indicate that a mutation at one end of the apoE3- NT four -helix bundle markedly enhan- ces the lipid binding...

Ngày tải lên: 30/03/2014, 11:20

... analyses of the chitin -binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1. Plant Mol Biol 56, 285–298. 39 Robertus JD & Monzingo AF (1999) The structure and action of ... On the other hand, PPL2 is a single -domain protein. Hence, PPL2 may possess at least two carbohydrate -binding sites. One of them probably corresponds to the cata- lytic...

Ngày tải lên: 16/03/2014, 13:20

... alog (pH ) pK a2 ), Lim1 is the lower limit of the curve, Lim 2 is the middle limit of the curve and Lim 3 is the upper limit of the curve. Stopped-flow kinetic studies of the oxidative half-reaction ... the expected mass of the a subunit to 40 421 Da, which is within error of the measured mass of 40 422 Da. Stopped-flow studies of the reductive half-reacti...

Ngày tải lên: 19/02/2014, 07:20

... at the end of an a-helical region of the HFE a1 domain (V100 and W103A) as being of par- ticular importance for TfR1 interactions [16,17,20]. The effect of HFE binding to TfR1 is to lower the affinity ... HFE, the proximity of the two a helices and the presence of amino acid side chains that project into the groove appear to prevent peptide binding [16]. The...

Ngày tải lên: 06/03/2014, 22:21

... (9), the development of the drinking event can be measured along the subparts of the glass of beer. Each subpart of the incremental theme argument is mapped onto a subpart of the 2 Complementary ... questioned the coherence of the class of achievement verbs, arguing that not all of them are non-durative. As noted above, Vendler identifies punctual events...

Ngày tải lên: 08/03/2014, 06:20

... expected, ablation of the DNA- binding activity within the N-terminal fragment of Cfp1 (amino acids 1–367, C169A), disruption of the Setd1A interaction with the C-terminal fragment of Cfp1 (amino ... restoration of normal levels of Setd1A, indicating that none of these Cfp1 domains are necessary for this rescue activity (Fig. 3B). Surpris- ingly, expression of...

Ngày tải lên: 15/03/2014, 09:20

... B -binding site. Its unmethylated nitrogen atom Ne2 donates the shortest of the three hydrogen bonds of the protein to the phosphate group of coenzyme B. The distance of 2.65 A ˚ indicates the ... because the genes required for the biosynthesis of the nickel cofactor F 430 [16] and for the post-translational modifications [9] are not yet known [17,18]. Also, the...

Ngày tải lên: 16/03/2014, 05:20

... of residual enzyme activity was used to determine the inhibition constant K i due to the tight binding of the protease by the inhibitor and the possibility of modification of the inhibitor bytheenzyme[32].Thesedatawerefittedtothetheoretical equation ... by the progress curve of the control reaction at pH 5.5 in the absence of testican-1 (A). When cathepsin L a...

Ngày tải lên: 17/03/2014, 10:20

... the formation of a closed form of the enzyme in order to be effi- ciently cleaved, whereas other substrates can be effec- tively utilized even by the open form of the enzyme. The possibility of ... for the model. Furthermore, the experimental effects observed for Tyr605 and Tyr612 mutants on enzyme activity validate the close proximity of these residues to the sub...

Ngày tải lên: 23/03/2014, 06:20

... suggests that cathepsin K is a new member of the kininase family. Both the kininogenase activity of cathepsin L and/or the kininase activity of cathepsin K may be favored by the generation of an acidic ... the kininogenase activity occurs after the partial hydrolysis of HMWK (Fig. 1), as reported for cystatin C-bound cathepsin L [37]. The pH-dependent kininogenas...

Ngày tải lên: 31/03/2014, 07:20